Team:Gifu/InterLab


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Inter Lab Study


Equipment

  • BioSHAKER TA-12R
  • use for shaking culture



  • Safire ART-NR:F129013
  • use for measurement of absorbance




Method of Measurement

      We constructed 5 plasmids [positive control, negative control, generator 1~3] by using BioBricks and then transformed to E. coli K-12 JM109. We checked them by colony direct PCR and plasmids sequence. We cultivated correct bacterias by shaking until OD660:0.5. Cultivated mediums were transferred to 96-well plate by 100mL. We read the absorbance.


      Setting of plate reader is following:




Result

  • Definition
  •   We expressed absorbance as relative value for positive control.
    This expression is following:


      Sample : Absorbance in sample
      NCav : Average of absorbance in negative control
      PCav : Average of absorbance in positive control



  • Value
  • Relative absorbance values for positive control are shown below.



    FIG1 Relative absorbance values
    20K : generator 1 (J23101 + I13504)
    22A : generator 2 (J23106 + I13504)
    22K : generator 3 (J23117 + I13504)




Discussion

      The value of device1 is much higher than that of device2 and 3. This is why each device is different from promoter sequence, especially -10 and -35 region.



    Promoter sequence in device1 resembles consensus sequence. On the other hand, device2 and 3 is not similar than device1. This is how each GFP expression is different.