Team:Gifu/calender


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CALENDER




May

21st

PCR and electrophoresis



22nd

PCR and electrophoresis



23rd

PCR and electrophoresis



24th

PCR and electrophoresis



26th

PCR and electrophoresis



27th

PCR and electrophoresis



28th

PCR and electrophoresis





June

1st

Gel extraction and DNA purification
Restriction enzyme processing and DNA purification



2nd

Ligation and transformation



3rd

Colony direct PCR



4th

PCR and electrophoresis
Plasmid extraction



5th

Electrophoresis for plasmid
PCR and electrophoresis
Gel extraction



6th

Restriction enzyme processing and DNA purification
Ligation and transformation



7th

Colony direct PCR
Plasmid extraction



9th

PCR and electrophoresis



10th

Gel extraction
DNA purification
PCR and electrophoresis
Restriction enzyme processing and DNA purification
Ligation and transformation



11th

Colony direct PCR



12th

Plasmid extraction



15th

Sequence analysis



17th

PCR and electrophoresis
DNA purification
Restriction enzyme processing
Ligation and transformation



18th

Colony direct PCR
Sequence analysis



23rd

PCR and electrophoresis



24th

PCR and electrophoresis
Restriction enzyme processing
DNA purification
Ligation and transformation



25th

Colony direct PCR



26th

Plasmid extraction
Preparation of the reagent
Colony direct PCR



27th

Plasmid extraction



29th

Plasmid extraction





July

1st

Plasmid extraction



8th

RNA extraction
Reverse transcription PCR
RNase processing
PCR and electrophoresis



9th

PCR and electrophoresis
DNA purification
Cultivation



10th

RNA extraction
Reverse transcription PCR
RNase processing
PCR and electrophoresis



17th

PCR and electrophoresis



19th

PCR and electrophoresis



21st

Cultivation



22nd

Cultivation



24th

Restriction enzyme processing
DNA purification
Ligation and transformation



25th

Colony direct PCR
Cultivation



26th

RNase processing
Reverse transcription PCR



27th

PCR and electrophoresis



28th

PCR and electrophoresis



31st

PCR and electrophoresis





August

1st

PCR and electrophoresis



2nd

Measurement of DNA concentration



5th

PCR and electrophoresis



6th

Gel extraction and purification
Restriction enzyme processing



7th

Colony direct PCR and electrophoresis
Cultivation



8th

Plasmid extraction
Ligation and transformation
Making competent cells



9th

Colony direct PCR and electrophoresis
PCR and electrophoresis



10th

PCR and purification



11th

Colony direct PCR and electrophoresis
Cultivation



12th

PCR and electrophoresis
Plasmid extraction
Checking capacity of competent cells



13th

DNA purification
Ligation and transformation



14th

Colony direct PCR and electrophoresis



18th

Plasmid extraction
PCR and electrophoresis and purification
Sequence analysis



19th

Colony direct PCR and electrophoresis



20th

Colony direct PCR and electrophoresis
Plasmid extraction
PCR and electrophoresis
Ligation and transformation



21st

Colony direct PCR and electrophoresis



22nd

Colony direct PCR and electrophoresis
Cultivation
PCR and electrophoresis



23rd

SDS-PAGE



24th

Colony direct PCR and electrophoresis



25th

Fluorescence measurement
PCR and electrophoresis



26th

Colony direct PCR and electrophoresis



27th

PCR and electrophoresis
Fluorescence measurement



28th

PCR and electrophoresis



29th

PCR and electrophoresis



30th

SDS-PAGE



31st

PCR and electrophoresis
Colony direct PCR and electrophoresis
Plasmid extraction
Cultivation





September

1st

PCR and electrophoresis
Plasmid extraction



2nd

Colony direct PCR and electrophoresis
Reverse transcription
PCR and electrophoresis
Sequence analysis



3rd

RNase processing
Reverse transcription PCR
PCR and electrophoresis and purification



4th

Electrophoresis and DNA purification
Restriction enzyme processing
PCR and electrophoresis
Ligation and transformation



5th

PCR and electrophoresis



6th

Colony direct PCR and electrophoresis



7th

SDS-PAGE
PCR and electrophoresis



8th

PCR and electrophoresis



9th

PCR and electrophoresis



10th

PCR and electrophoresis



11th

PCR and electrophoresis



12th

PCR and electrophoresis



13th

Data analysis