|
|
TEAM PROFILE
| Team Name: | Gifu | | Primary Contact (PI): | Hitoshi IWAHASHI | | Schools: | Gifu University
Yanagido, Gifu, Japan | | Division: | iGEM | Application Date: 2015-04-27 | | Section: | Undergraduate | | Region: | Asia | Acceptance Date: 2015-05-02 08:32:07 | | Description: |
|
|
Welcome to iGEM 2015 ! Your iGEM 2015 team application was accepted by iGEM Headquarters on 2015-05-02 08:32:07
and your team registration fee has been received. |
| Assigned Track: New Application
|
Circular mRNA ver.2.0
| In last year, we succeeded in designing the sequence which synthesizes circular mRNA and long chain protein in Escherichia coli.
In this year, we had 2 purposes in our study. One was an efficiency of the circularization. The efficiency was lower in our previous study. This was why the splicing was hard to happen because two sequences to act as ribozyme for splicing were far each other. So we incorporated complementary sequences around the ribozyme regions. We thought that the treatment brought two regions close and the efficiency of the circularization improved.
The other was to synthesize useful proteins. In our previous study, synthesized long-chain proteins lose their function because the folding of the proteins was broken. So we incorporated linker sequences into circular mRNA to synthesize the functional long-chain protein.
|
| This range of part numbers has been assigned to your team for use during the summer: BBa_K1859000 to BBa_K1859999
|
| 
DNA Kit of Parts shipping information has been entered, thanks.
View shipping information