Team:HUST-China/Part Collection

Team:HUST-China:Results


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Best Part Collection:

This year, we submitted 26 new parts in total, including basic parts and composite parts. All the parts we used in our Euk.cement project were completely submitted and shipped. Particularly, we did well in the standardization of E.coli 50S ribosomal protein (Si-tag) with the different combination of domains: 1, 2, 3, 1+2,1+3,2+3,1+2+3,1L3.

We added a signal peptide and cell wall protein respectively at N-erminal and C-terminal of the Si-tags which can immobilize the working protein onto cell wall to promote the protein’s function.

The results of immunofluorescence assay showed that we succeeded in displaying the silica-tag onto the cell surface. Silica surface binding test shows each protein domain had different binding ability, so that we can choose different combinations of Si-tag domains to satisfy different requirement of binding intensity in further real utilization.

 
Part Number Description Nick Name
BBa_K1592007 LIP prepro + E. coli ribosomal protein L2 (1-60) + YLcwp3 Fusion Si-tag1
BBa_K1592008 LIP prepro + E. coli ribosomal protein L2 (61-202) + YLcwp3 Fusion Si-tag2
BBa_K1592009 LIP prepro + E. coli ribosomal protein L2 (203-273) + YLcwp3 Fusion Si-tag3
BBa_K1592010 LIP2 prepro + E. coli ribosomal protein L2 (1-202)+ YLcwp3 Fusion Si-tag12
BBa_K1592011 LIP prepro + E. coli ribosomal protein L2 (61-273) + YLcwp3 Fusion Si-tag23
BBa_K1592012 LIP prepro + E. coli ribosomal protein L2 (1-60,203-273) + YLcwp3 Fusion Si-tag13
BBa_K1592013 LIP prepro + E. coli ribosomal protein L2 (1-60,GS linker,202-273) + YLcwp3 Si-tag1L3
BBa_K1592014 LIP prepro + E. coli ribosomal protein L2 (1-273) + YLcwp3 Si-tag123