Team:Hong Kong HKU/Modeling
|
MODELING
Module 1
1.1) Transcription rate of AraC, assuming the diffusion rate of arabinose across the cell is constant
1.2) Transcription rate of CI repressor; pBAD as positive inducible promoter
1.3) Transcription rate of Cas protein
Module 2
2.1) Transcription rate of trpR, assuming the diffusion rate of tryptophan across the cell is constant
2.2) Transcription rate of sgRNA
2.3) Target DNA (dnaE gene) cleavage efficiency by cas-sgRNA complex
| Parameters | Description |
|---|---|
| tr | Transcription Rate |
| tl | Translation Rate |
| D | Degradation Rate |
| n | Hill coefficient |
| Arain | L-arabinose within the cell |
| AraC-Arain | AraC with L-arabinose in the cell |
| Trpin | L-tryptophan within the cell |
| trpR-Trpin | trpR with L-tryptophan in the cell |
| Cas-sgRNA | Cas protein with sgRNA |
| Km | Concentration of substrate that allows enzyme to achieve Vmax/2 |