Team:Jilin China/Collaborations

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Improve the project of WHU-China in2014

Experiment background:
iGEM encourages us to communicate and cooperate with other teams. We found that the project conducted by Wuhan University (click here to see more details about  WHU-China 2014 Project) last year was meaningful and of significance to environment and human health. Unfortunately, they didn’t finish their project successfully. Therefore, we analyze Wuhan University’s last project and make some improvements this year. They are also willing to collaborate with us and have already sent us their pSB1C3 vector in their detection system (click here to see more details about  WHU-China 2015 Collaborations). We start our project based on this part. 

Purpose:
After our confirming experiment, we found that the reason for their failure is mainly because the hxlR promoter’s starting ability is too weak and their system will do harm to the bacteria’s metabolic system. So we decided to change the promoter in their cleaning system and we add T7 RNA polymerase gene in the detection system. By doing this, we are able to reduce the unnecessary expression in the cells and make the whole system work well.

 

Materials:

Name	Source
pSB1C3 vector	Wuhan University
T7 RNA polymerase	Pro.Gao’s lab
FDH	Pro.Gao’s lab
PADH	Pro.Gao’s lab