We have characterized the part BBa_J64997 (T7 promoter sequence, 2007 Voigt Lab).
COWg Fragments
COWg1
T7- CooA- 2½ TT
1,000 bp
COWg2
2½ TT- ⅔ BSMT
1,050 bp
COWg3
⅓ BSMT-⅓ pchBA
1,050 bp
COWg4
⅔ pchBA-pCoof
1,222 bp
Plasmids
PCR 2.1 Plasmid
T7- CooA- 2½ TT
3,931 bp
pCDF-T7-gfp Plasmid
2½ TT- ⅔ BSMT
4,279 bp
pCDF-pCoof-gfp Plasmid
⅓ BSMT-⅓ pchBA
4,308 bp
pET21
⅔ pchBA-pCoof
5,731 bp
Other Parts
Promoters
T7
23 bp
pCoof
94 bp
RBS
12 bp
Genes
CooA
669 bp
BSMT
1,074 bp
pchBA
1,736 bp
sfGFP
744 bp
Other
TT
129 bp
New Parts
pCooF-RBS
Short Description: promoter and rbs, activated by CooA transcription factor
Long Description: part of the pCooF operon and ribosome binding site downstream, activated by the CooA transcription factor bound to carbon monoxide.
T7-Wintergreen
Short Description: continual expression of a wintergreen fragrance
Long Description: the wintergreen pathway under constituent promoter T7. pchBA and BMST genes convert chorismate to salicylic acid the methyl salicylate, the desired wintergreen fragrance.
Whole Construct
Short Description: responds to presence of carbon monoxide with wintergreen smell
Long Description: CooA transcription factor binds carbon monoxide, and this CooA-CO complex activates the pCooF promoter, which regulates expression of the wintergreen pathway. The wintergreen pathway consists of the pchBA and BSMT genes. This pathway converts chorismate, an endogenous molecule in E. coli bacteria to salicylic acid with the pchBA protein, and salicylic acid to methyl salicylate, or the wintergreen fragrance, with the BSMT protein. Thus in the presence of carbon monoxide, this part creates a wintergreen fragrance.
