Team:London Biohackspace/Design
We want to introduce a whole new group of people to the DIYBio community using the exciting products of synthetic biology. The DIYBio community is still a nascent one, and we believe it has the potential to become a big thing (hopefully a really big thing). The SynBio Brewery DIY Brew Kit is therefore designed to get members of the home-brewing and micro-brewing community interested in DIYBio and synthetic biology - who better to get involved, than people that are already familiar with manipulating biology to do their bidding? Our project is not about increasing crop yield or increasing shelf life of crops. We are trying to make synthetic biology and genetic modification easy to understand by making the changes "easy to see" in the product. We believe that without given the opportunity to learn what synbio is all about - the public wouldn't be able to decide whether they want to use this technology in the future or not. We aimed to involve the public in synthetic biology in a fun and engaging in way! We have designed a number of strains as a starting point for the yeast library that brewers can use to easily enhance the flavour, smell, colour and nutritional content of their beer: Miraculin is a protein found naturally in the fruits of the Miracle Berry plant Synsepalum dulcificum and has been found to make sour foods taste sweeter. The taste altering property of Miraculin therefore means it has potential use as an artificial sweetener and makes for a healthier alternative to natural sugars found in most products. We have designed a yeast strain expressing the miraculin protein which will create a beer with this interesting flavour additive.
Related part: BBa_K1845002 Lycopene is a carotenoid compound that can be found in tomatoes and many other red fruits. We have designed a yeast strain expressing the three enzymes (CrtE, CrtB, CrtI) responsible for the biosynthesis of lycopene which will create red pigmented beer. Research into the nutritional value of lycopene also suggests that it acts as an antioxidant and therefore has a number of health benefits when consumed as part of a balanced diet. Additionally, this strain provides a basis for producing a yeast strain that synthesises beta-ionone, a woody flavour compund, as lycopene is a precursor in beta-ionone's synthesis. The yeast alcohol acetyltransferase gene ATF1 is responsible for regulating the production of a broad range of volatile compounds by yeast during fermentation. We have designed a yeast strain with an overexpressed copy of ATF1 derived from Saccharomyces carlsbergensis which will create a beer with boosted fruity notes.
Related part: BBa_K1845003 OneProt2 is a synthetic protein based on the principle of Team SDU-Denmark 2014's OneProt protein. It contains all the essential amino acids (in the WHO recommended ratio) required in human diet. We have designed a yeast strain expressing the OneProt2 protein which will create a beer with improved nutritional element.
Related part: BBa_K1845004 We have great hope that brewers and already established members of the DIYBio community will want to create new strains of yeast and add to our open source strain library. TO facilitate this, we have designed a set of genetic parts and protocols to enable the community to more easily produce new and interesting yeast strains from existing commercially available brewing strains. Additionally, these open-source tools for working with yeast are primarily designed to work in a community lab setting, thus we hope to lower the entry barrier for new and existing community labs looking to participate in iGEM as well as making it easier for community labs to share resources. An easy, cheap and automated (convenient) method for preparing competent cells in a community lab without a -80 freezer. More... We have developed an easy and cheap method for assembling vectors without the need for expensive purified restriction enzymes and ligases. A great method for community labs to lower the cost of constructing vectors. More... We have developed a set of genetic parts that will allow users to modify the expression levels of genes inserted into yeast via RNA interference so that the novel brewing strains can be tuned. More... We have developed protocols for producing auxotroph mutants of existing brewing strains. The first step in creating novel brewing yeast strains is to modify an existing strain that has either been purchased from a home brew supplier or isolated from a bottle of beer so that it becomes auxotrophic for a particular key nutrient. This is an essential step as it provides a means to select subsequent strains that have been further genetically modified. We have designed a set of genes parts that enable the user to create auxotrophic mutants lacking the ability to synthesize uracil and leucine that are key amino acids required for protein synthesis. These mutant strains will therefore only grow on a media containing uracil or leucine or via further genetic modification which restores the strains ability to synthesize these amino acids.The Motivation
What is the Synbio Brewery DIY-Brewkit
The SynBio Brewery DIY-Brewkit is a package containing everything an established home-brewer or micro-brewer needs to start making beer with a synbio twist. Each basic package contains six GM brewing yeast strains - each of which adds one novel property to the beer brewed with it. We hope brewers will start to experiment with different combinations and quantities of our yeast strains to produce truly unique beers. Each kit also contains a simple explanation of what each strain does and the science behind it as well as resources to help the brewers dig deeper into how to go about making new strains themselves as DIY-biologists. More about the design of the kit.
scroll through the pages to check out the booklet
The Strains
Miraculin expression
Lycopene biosynthesis
ATF1 overexpression
OneProt2 expression
Community resources for growing the brew kit yeast library
Filtration method for competent cells preparation
SPliCE vector assembly
RNA interference based regulation
Creating Leucine auxotrophic S. cerevisiae strains
