Team:Marburg/Protocols/Protocol15
Preparation of Mini Cells
- grow cells in LB medium @ 37 °C
- harvest cells when OD = 0.5
- centrifuge (30 min, 4000 rpm, 4 °C)
- resuspend pellet in 1/100 of the culture volume in M9 minimal medium without carbon source
- separate mini cells by centrifugation through a 10%-30% sucrose gradient
- transformation into chemocompetent cells (see transformation into chemocompetent (RbCl) cells)
