Team:Marburg/Protocols/Protocol19
Transformation into yeast
- fill 40 µL (max. 80 µL) of competent yeast cells in a 1.5 mL Eppendorf tube (store on ice!)
- add DNA to the tube
- mix thoroughly through pipetting
- shock cells with 1,500 V for 5 ms.
- add 700 µL 1 M Sorbitol
- plate on SD-URA plates
- incubate plates @ 30 °C for about 3 days
