Team:Marburg/Protocols/Protocol29
Ligation
Prepare the following reaction mixture (total volume: 20 μL):
| Ligation Mix | |
|---|---|
| 100 ng | Vector DNA |
| 300 ng | Insert DNA |
| 2 μL | T4 DNA Ligase Buffer |
| 1 μL | T4 DNA Ligase |
| X μL (fill up to 20 μL) | dd H2O |
Incubate for 30 min at 37°C
