Team:NYU-AD/Notebook/Week4

Exterminator Coli

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Week 4

26/08/15

• Performed ligation of the promoter and tnaA
• Amplifying the tnaA and tnaB sequence. .
• Added 200ul of water (DNA- RNA free) into each tube
• Performed the Nano Drop . The concentration of both tubes is approximately 7-8ug/ul.

27/08/2015

• Nanodrop to verify concentration of resuspended DNA
• Nuclease- free H2O used to resuspend the DNA was found to be contaminated with 4.8ug/ul of DNA
• The actual DNA content of plasmids are around 6-8 ug/ul on average
• We decided to proceed with the transformation since protocol specified 10ug- 100ug of DNA.

28/08/2015

• We received the tnaa and the tnab tubes from IDT on the 26/08/15
• Resuspended the tnaa and the tnab sequence by adding 200ul of water ( DNAse and RNAase free) into each tube.

29/08/2015

• Nanodropped the DNA to verify the concentration of resuspended DNA. The Nuclease-free water that was used to reuspend the DNA was found to be contaminated with 4.8ng/ul of DNA. The actual DNA content of the plasmids is around 6-8ng/ul on average.
• We still proceeded with the transformation of the DNA since the protocol specified 10ug-1000ug of DNA.