Team:UIUC Illinois/Results

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Results:

Despite encountering high levels of mutations throughout our various attempts to make a standardized version of the SCRIBE system, on the final day before our parts were due, we finally saw signs of successful recombination in response to IPTG!

Results that constitute a characterization of our part can be found on its registry page, located here.

Unsuccessful Results:

We were unable to obtain a functional construct of SCRIBE without the lac promoter

Because of this we were unable to carry out our original goal of examining SCRIBE's potential biosensing applications

Future plans:

Add other promoters upstream of the SCRIBE cassette to expand its possible applications, particularly in environmental biosensing applications, such as groundwater contamination.

Combine SCRIBE with the genome-editing tools to function in eukaryotic organisms

Further examine potential mutagenic effects related to the overproduction of msDNA and the impact it may have had on our project [1].

[1] Mao JR1, Inouye S, Inouye M. "Enhancement of frame-shift mutation by the overproduction of msDNA in Escherichia coli." FEMS Microbiol Lett. 1996 Oct 15;144(1):109-15.