View source for Team:Paris Bettencourt/Protocols/Gel Electrophoresis

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    <td><b>Gel-Electrophoresis protocol</b><br />
    <ul>
      <li>Preparation of the Gel (50mL)
        <ul>
          <li>Mix 0.5 grams of agarose with 50mL 0.5% TAE solution</li>
          <li>Heat the Erlenmeyer (conical flask) in a microwave until the liquid becomes totally transparent.</li>
          <li>Let it rest until you can take the Erlenmeyer in your hand without pain for 10 seconds.</li>
          <li>Add 5uL of SYBR® Safe (10000X) and mix gently until the solution homogenizes.</li>
          <li>Pour the liquid in a gel mold with the desired number of wells and wait 20-30 min (or until the gel is solidified).</li>
          <li>Put the gel inside the electrophoresis machine and pour TAE 0.5% until the gel is totally submerged.</li>
        </ul>
      <li>Preparation of the samples</li>
        <ul>
          <li>Mix 5uL of each sample with 1uL of 6X loading dye in microcentrifuge tubes/ on a piece of parafilm / in PCR tubes.</li>

      <li>Pipette 5-6uL of each sample inside a different well.</li>
      <li>Pipette 5uL of the appropriate ladder in one of the wells.</li>
      <li>Close the machine and launch the migration with the desired voltage</li>
      <li>After the desired migration time turn of the machine, remove the lid and put the gel inside the Blue light revelator.</li>
        </ul>
     </ul>
    </td>
  </tr>
</table>
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