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| | <script src="https://2015.igem.org/Template:UNITN-Trento/JSscrollTo?action=raw&ctype=text/javascript"></script> | | <script src="https://2015.igem.org/Template:UNITN-Trento/JSscrollTo?action=raw&ctype=text/javascript"></script> |
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| − | <link rel="stylesheet" type="text/css" href="http://www.startrekfederation.it/transfer_area/team.css?action=raw&ctype=text/css" /> | + | <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:UNITN-Trento/CSSanimate?action=raw&ctype=text/css"></link> |
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| | {{UNITN-Trento/mainMenu}} | | {{UNITN-Trento/mainMenu}} |
| − | <html> | + | <html><script>jQuery('#menulin_more, #menulin_more_safety').addClass('current');</script></header> |
| − | <script>jQuery('#menulin_team').addClass('current');</script>
| + | |
| − | </header>
| + | |
| | <!-- Main --> | | <!-- Main --> |
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| − |
| + | <section id="cta" data-stellar-background-ratio="0.4" style="background-image:url('https://static.igem.org/mediawiki/2015/6/65/Unitn_pics_cta_safety.jpg');"> |
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| − | <section id="cta" data-stellar-background-ratio="0.4" style="background-image:url('https://static.igem.org/mediawiki/2015/8/89/Unitn_pics_cta_notebook.jpg');"> | + | |
| | | | |
| | <header> | | <header> |
| − | <h2><strong>Meet the UNITN Team</strong></h2> | + | <h2><strong> Safety </strong></h2> |
| − | <p>Alessandro, Elisa, Claudio, Silvia, Veronica, Riccardo and Moreno</p> | + | <p> Work hard, work safe </p> |
| | </header> | | </header> |
| | </section> | | </section> |
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| | <!-- BEGIN iGEM_Content_Section --> | | <!-- BEGIN iGEM_Content_Section --> |
| | <article id="main"> | | <article id="main"> |
| − |
| + | <section class="wrapper style1 container special" style="margin-top:3em;"> |
| − | <section class="wrapper style4 container" style="margin-top:1em;">
| + | |
| − |
| + | <div class=" 4u 12u(narrower)"> |
| − | <h2> Team Members </h2>
| + | |
| − |
| + | <section> |
| − | <div class="row teamEntry"> | + | <header> |
| − | <div class=" 3u 12u(narrower) team_image"> | + | <div href="#" class="rotate-box square-icon"><span class="rotate-box-icon"><i class="faa flaticon-bacteria"></i></span></div> |
| − | <div class="team_image_container style1" style="background-image:url('https://static.igem.org/mediawiki/2015/a/a0/Unitn_pics_team_alessandro.jpg');"></div>
| + | </header> |
| − | <div style="margin-top:0.8em;"> | + | <p>We have reviewed our organisms and parts against the <a class="i_linker" href="https://2015.igem.org/Safety/WhiteList" target="_blank">White List</a>. All the parts and bacterial strains that we used are in the <span class="i_enph">Risk Group 1</span></p> |
| − | <a href="https://www.facebook.com/alessandro.turcato.1" target="_blank" class="icon-button facebook"><i class="fa fa-facebook"></i><span></span></a>
| + | </section> |
| − | <a href="mailto:alessandro.turcato@studenti.unitn.it" target="_blank" class="icon-button mail"><i class="fa fa-envelope"></i><span></span></a>
| + | |
| − | </div> | + | |
| − | </div>
| + | |
| − | <div class="9u 12u(narrower) team_content">
| + | |
| − | <p class="main_name wikicolor ">Alessandro Turcato</p>
| + | |
| − | <p>Hi.. I’m Alessandro Turcato I’m from Marano Vicentino in Veneto (Italy). I’m attending the third year of "Biomolecular sciences and technologies" B.Sc. in Trento. I really enjoy playing guitar and playing football furthermore I’m very happy to join the IGEM competition. I’m mainly involved into MFC part of our project and into envelope our own prototype. I hope you guys enjoy our work… see you in Boston!!</p>
| + | |
| − |
| + | |
| − | <p class="skill_list wikicolor"><span>Skills:</span>
| + | |
| − | <i class="fli flaticon-orbital"></i> <span>Physics of the Group </span>
| + | |
| − | <i class="fli flaticon-kitchenpack2"></i> <span>Passionate Lover</span>
| + | |
| − | </p>
| + | |
| − |
| + | |
| − | </div> | + | |
| | </div> | | </div> |
| − |
| + | <div class="4u 12u(narrower)"> |
| − | <div class="row teamEntry right"> | + | |
| − | <div class="3u 12u(narrower) team_image">
| + | <section> |
| − | <div class="team_image_container style2" style="background-image:url('https://static.igem.org/mediawiki/2015/6/6e/Unitn_pics_team_elisa.jpg');"></div>
| + | <header> |
| − |
| + | <div href="#" class="rotate-box square-icon"><span class="rotate-box-icon"><i class="faa flaticon-checkmark11"></i></span></div> |
| − | <div style="margin-top:0.8em;"> | + | </header> |
| − | <a href="https://www.facebook.com/elisa.godino.10" target="_blank" class="icon-button facebook"><i class="fa fa-facebook"></i><span></span></a>
| + | <p>We have submitted to iGEM headquarters <span class="i_enph">“About Our Lab”</span> and <span class="i_enph">“About Our Project”</span> questionnaires by the June 26<sup>th</sup> deadline</p> |
| − | <a href="https://twitter.com/elisa_godino" target="_blank" class="icon-button twitter"><i class="fa fa-twitter"></i><span></span></a>
| + | </section> |
| − | <a href="mailto:elisa.godino@studenti.unitn.it" target="_blank" class="icon-button mail"><i class="fa fa-envelope"></i><span></span></a>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="9u 12u(narrower) team_content">
| + | |
| − | <p class="main_name wikicolor ">Elisa Godino</p> | + | |
| − | <p>Hi! I'm Elisa Godino, I'm 22 years old and I was born in Cles (TN Italy). I recently graduated in Biomolecular Sciences and Technology at the University of Trento. I love science and I'm having a great time with this incredible iGEM experience. I also like going to the lake with friends and play beach volley. Sometimes I like to make cakes to the delight of my teammates! Mainly I deal with the Measurement Interlab Study and with Human Practices.</p> | + | |
| − |
| + | |
| − | <p class="skill_list wikicolor"><span>Skills:</span>
| + | |
| − | <i class="fli flaticon-dna15"></i> <span>Queen of Cloning </span>
| + | |
| − | <i class="fli flaticon-caffeine1"></i> <span>Sweetheart</span>
| + | |
| − | </p>
| + | |
| − |
| + | |
| − | </div> | + | |
| | </div> | | </div> |
| | + | <div class="4u 12u(narrower)"> |
| | + | |
| | + | <section> |
| | + | <header> |
| | + | <div href="#" class="rotate-box square-icon"><span class="rotate-box-icon"><i class="faa flaticon-verified7"></i></span></div> |
| | + | </header> |
| | + | <p>We have submitted to iGEM headquarters the <span class="i_enph">“Final Safety Form”</span> by the August 28<sup>th</sup> deadline</p> |
| | + | </section> |
| | | | |
| − | <div class="row teamEntry">
| |
| − | <div class="3u 12u(narrower) team_image">
| |
| − | <div class="team_image_container style3" style="background-image:url('https://static.igem.org/mediawiki/2015/d/d4/Unitn_pics_team_claudio.jpg');"></div>
| |
| − |
| |
| − | <div style="margin-top:0.8em;">
| |
| − | <a href="https://www.facebook.com/claudio.osspegorar" target="_blank" class="icon-button facebook"><i class="fa fa-facebook"></i><span></span></a>
| |
| − | <a href="https://twitter.com/dudepartyrock" target="_blank" class="icon-button twitter"><i class="fa fa-twitter"></i><span></span></a>
| |
| − | <a href="mailto:claudio.osspegorar@studenti.unitn.it" target="_blank" class="icon-button mail"><i class="fa fa-envelope"></i><span></span></a>
| |
| − | </div>
| |
| − |
| |
| − | </div>
| |
| − | <div class="9u 12u(narrower) team_content">
| |
| − | <p class="main_name wikicolor ">Claudio Oss Pegorar</p>
| |
| − | <p>Hi! I am Claudio Oss Pegorar, I am 22 and I was born in Trento (Italy). I am studying "Biomolecular sciences and technologies" as a B.Sc. student at the University of Trento. What I like to do other than staying in the lab is playing the guitar, day tripping and hanging out with my friends. I am grateful I had the chance to participate and contribute to this project and I enjoy every single day as an iGEMer. I am mainly involved into lab work, InterLab Study work and Safety.</p>
| |
| − |
| |
| − | <p class="skill_list wikicolor"><span>Skills:</span>
| |
| − | <i class="fli flaticon-translation"></i> <span>Human Translator</span>
| |
| − | <i class="fli flaticon-lightbulb28"></i> <span>Fluorescence Man</span>
| |
| − | </p>
| |
| − | </div>
| |
| | </div> | | </div> |
| | + | </div> |
| | + | </section> |
| | + | |
| | + | |
| | + | <!-- One --> |
| | + | <section class="wrapper style4 container" style="margin-top:1em;"> |
| | | | |
| − | <div class="row teamEntry right"> | + | |
| − | <div class="3u 12u(narrower) team_image"> | + | <div class="content"> |
| − | <div class="team_image_container style4" style="background-image:url('https://static.igem.org/mediawiki/2015/0/0d/Unitn_pics_team_silvia.jpg');"></div> | + | <section> |
| − |
| + | <header> |
| − | <div style="margin-top:0.8em;">
| + | <h3>About Safety</h3> |
| − | <a href="https://www.facebook.com/galsilvia" target="_blank" class="icon-button facebook"><i class="fa fa-facebook"></i><span></span></a> | + | </header> |
| − | <a href="https://twitter.com/silviagalvan93" target="_blank" class="icon-button twitter"><i class="fa fa-twitter"></i><span></span></a>
| + | |
| − | <a href="mailto:silvia.galvan@studenti.unitn.it" target="_blank" class="icon-button mail"><i class="fa fa-envelope"></i><span></span></a>
| + | <p>We, the UNITN iGEM Team want to follow high standards of safe and responsible biological engineering. Before entering the lab we attended mandatory on-line courses regarding lab safety with final tests. In particular we focused on the following topics:</p> |
| − | </div> | + | <ul class="checklist"> |
| − |
| + | <li>General considerations on risks and prevention</li> |
| − | </div>
| + | <li>Electrical Hazard</li> |
| − | <div class="9u 12u(narrower) team_content">
| + | <li>Chemical Hazard</li> |
| − | <p class="main_name wikicolor">Silvia Galvan</p> | + | <li>Biological Hazard</li> |
| − | <p> I’m Silvia Galvan, I’m 22 years and I was born in Borgo Valsugana (Trento), where I live with my family. I am currently a student of the Bachelor of Science in Biomolecular Sciences and Technology at the University of Trento. My passion is running and I do Athletics since I was a child. I enjoy spending time with my two lovely nephews, and with my teammates of course! We’re having such a great summer in the lab , the work is hard sometimes but we have lots of fun together. I feel really lucky to have had the possibility to be an IGEMer and to have found such a great team! I am mainly dealing with the proteorhodopsin part. | + | <li>Compressed gasses and cryogenic liquids</li> |
| − | Fun fact: I have a dog named Brick, sometimes I call him BioBrick!</p>
| + | </ul> |
| − |
| + | <p>We also attended the “Health and safety at the workplace” course, which is mandatory for all students and workers at the University of Trento.</p> |
| − | <p class="skill_list wikicolor"><span>Skills:</span> | + | </section> |
| − | <i class="fli flaticon-science29"></i> <span>pH Princess</span>, | + | |
| − | <i class="fli flaticon-dessert54"></i> <span>Hearty Eater</span> | + | |
| − | </p> | + | |
| − |
| + | |
| − | </div> | + | |
| | </div> | | </div> |
| − |
| + | |
| − | <div class="row teamEntry">
| + | </section> |
| − | <div class=" 3u 12u(narrower) team_image"> | + | |
| − | <div class="team_image_container style5" style="background-image:url('https://static.igem.org/mediawiki/2015/6/66/Unitn_pics_team_riccardo.jpg');"></div> | + | <section class="wrapper style4 container" style="margin-top:1em;"> |
| | + | |
| | + | <!-- Content --> |
| | + | |
| | + | <section> |
| | + | <!--<a href="#" class="image featured"><img src="images/pic04.jpg" alt="" /></a>--> |
| | + | <header> |
| | + | <h3>Safe Project Design</h3> |
| | + | </header> |
| | + | <!-- TODO --> |
| | | | |
| − | <div style="margin-top:0.8em;"> | + | <div class="row"> |
| − | <a href="https://www.facebook.com/riccardo.corbellari" target="_blank" class="icon-button facebook"><i class="fa fa-facebook"></i><span></span></a> | + | <div class="6u 12u(narrower)"> |
| − | <a href="https://twitter.com/RCorbellari" target="_blank" class="icon-button twitter"><i class="fa fa-twitter"></i><span></span></a>
| + | <section> |
| − | <a href="mailto:riccardo.corbellari@studenti.unitn.it" target="_blank" class="icon-button mail"><i class="fa fa-envelope"></i><span></span></a>
| + | <header> |
| − | </div>
| + | <p class="question">Who will use your product? What opinions do these people have about your project?</p> |
| − |
| + | </header> |
| − | </div>
| + | <p>Answer</p> |
| − | <div class=" 9u 12u(narrower) team_content"> | + | </section> |
| − | <p class="main_name wikicolor ">Riccardo Corbellari</p>
| + | </div> |
| − |
| + | <div class=" 6u 12u(narrower)"> |
| − | <p> Hi! I’m Riccardo Corbellari, I was born in Verona and I’m 21. I’m a student of “Science in Biomolecular Sciences and Technologies” at the University of Trento. I thought I was an only child, but during this summer I found out I have 3 sisters and 3 brothers: my iGEM family! I love playing handball and watching movies. I like to fool around, for example I can’t stop teasing Alessandro, but when it’s work time no one’s more serious than me. Mainly i’m involved in pncB construction, in the pMFC realization and into designing our prototype. To sum up: how much is 15 and 18?</p> | + | <section> |
| − |
| + | <header> |
| − | <p class="skill_list wikicolor"><span>Skills:</span>
| + | <p class="question"> Where will your product be used? On a farm, in a factory, inside human bodies, in the ocean?</p> |
| − | <i class="fli flaticon-science8"></i> <span>Captain of Sequencing</span>
| + | </header> |
| − | <i class="fli flaticon-man282"></i> <span>Lab Motivator</span>
| + | <p>Answer</p> |
| − | </p>
| + | </section> |
| − |
| + | </div> |
| − | </div>
| + | </div> |
| − | </div>
| + | |
| − |
| + | |
| − | <div class="row teamEntry right">
| + | |
| − | <div class="3u 12u(narrower) team_image">
| + | |
| − | <div class="team_image_container style6" style="background-image:url('https://static.igem.org/mediawiki/2015/a/a2/Unitn_pics_team_pina.jpg');"></div> | + | |
| | | | |
| − | <div style="margin-top:0.8em;"> | + | <div class="row"> |
| − | <a href="https://www.facebook.com/veronica.pinamonti" target="_blank" class="icon-button facebook"><i class="fa fa-facebook"></i><span></span></a> | + | <div class="6u 12u(narrower)"> |
| − | <a href="https://twitter.com/VeronicaPina93" target="_blank" class="icon-button twitter"><i class="fa fa-twitter"></i><span></span></a> | + | <section> |
| − | <a href="mailto:veronica.pinamonti@studenti.unitn.it" target="_blank" class="icon-button mail"><i class="fa fa-envelope"></i><span></span></a>
| + | <header> |
| | + | <p class="question">If your product is successful, who will receive benefits and who will be harmed?</p> |
| | + | </header> |
| | + | <p>Answer</p> |
| | + | </section> |
| | + | </div> |
| | + | <div class="6u 12u(narrower)"> |
| | + | <section> |
| | + | <header> |
| | + | <p class="question">What happens when it's all used up? Will it be sterilized, discarded, or recycled?</p> |
| | + | </header> |
| | + | <p>Answer</p> |
| | + | </section> |
| | + | </div> |
| | </div> | | </div> |
| | | | |
| − | </div>
| + | <div class="row"> |
| − | <div class=" 9u 12u(narrower) team_content"> | + | <div class="6u 12u(narrower)"> |
| − | <p class=" main_name wikicolor"> Veronica Pinamonti</p> | + | <section> |
| | + | <header> |
| | + | <p class="question">Is it safer, cheaper, or better than other technologies that do the same thing?</p> |
| | + | </header> |
| | + | <p>Answer</p> |
| | + | </section> |
| | + | </div> |
| | + | <div class=" 6u 12u(narrower)"> |
| | + | <section> |
| | + | <header> |
| | + | <p class=" question"> __</p > |
| | + | </header> |
| | + | <p>Answer</p> |
| | + | </section> |
| | + | </div> |
| | + | </div> |
| | + | </section> |
| | + | </div> |
| | + | </section> |
| | + | |
| | + | |
| | + | <section class="wrapper style4 container" style="margin-top:1em;"> |
| | + | |
| | + | <!-- Content --> |
| | + | <div class="content"> |
| | + | <section> |
| | + | <header> |
| | + | <h3>Working Safely... with Parts</h3> |
| | + | </header> |
| | + | <!-- TODO --> |
| | | | |
| − | <p>I am Veronica, I was born in Cles, a small town in a small valley near Trento. I’m attending the third year in ‘Biomolecular Sciences and Technology’ B.Sc. in Trento. I usually spend my free time with my friends at the lake. I really love science and my teammates (more or less) and this summer I’m having a great time in the lab with them: we laugh hard but work harder. Mainly I’m involved in the retinal expression in E.coli. I hope you will appreciate and enjoy our project as much as I did.</p> | + | <p>All the parts we used throughout the project are NOT toxic or dangerous for humans or the environment. We neither worked with proteins toxic themselves, nor we used enzymes that synthesize toxic molecules. In addition to basic parts from the registry (i.e. RBS, promoters, β-carotene, etc), we also used two genes from the SAR86 uncultured bacteria. These genes are proteorhodopsin, which was extracted from the Registry (BBa_K773002) and blh (15,15’-β-carotene dioxygenase), which was synthesized by Genescript. There is no associated risk level with the uncultured bacteria SAR86, however we are using only DNA sequences that encodes for genes, which carry distinct functions that are no harmful to humans, or the environment. We have clarified this issue with iGEM Safety Office.</p> |
| − |
| + | <p>We did <span class="i_enph">NOT</span> use any virulence factors in our organisms.</p> |
| − | <p class="skill_list wikicolor"><span>Skills:</span> | + | <p>The parts we used are to be considered safe as single parts and also as combined. Environmental dispersion of those parts would not represent a biological hazard or danger.</p> |
| − | <i class="fli flaticon-laboratory4"></i> <span> Spectra Manager</span>
| + | |
| − | <i class="fli flaticon-man182"></i> <span> Just Pina!</span>
| + | |
| − | </p> | + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <div class="row teamEntry">
| + | |
| − | <div class="3u 12u(narrower) team_image">
| + | |
| − | <div class="team_image_container style6" style="background-image:url('https://static.igem.org/mediawiki/2015/7/7c/Unitn_pics_team_moreno.jpg');"></div>
| + | |
| | | | |
| − | <div style="margin-top:0.8em;"> | + | <header> |
| − | <a href="https://www.facebook.com/digitaldestiny" target="_blank" class="icon-button facebook"><i class="fa fa-facebook"></i><span></span></a> | + | <h3>Working Safely... with Organisms</h3> |
| − | <a href="https://twitter.com/Azufre451" target="_blank" class="icon-button twitter"><i class="fa fa-twitter"></i><span></span></a> | + | </header> |
| − | <a href="mailto:moreno.zolfo@studenti.unitn.it" target="_blank" class="icon-button mail"><i class="fa fa-envelope"></i><span></span></a>
| + | <p>All the bacterial strains used are from Risk Group 1. We used the following strains:</p> |
| − | </div> | + | <ul class="checklist"> |
| − | </div>
| + | <li> One Shot® TOP10 Chemically Competent E. coli</li> |
| − | <div class="9u 12u(narrower) team_content">
| + | <li> K12 - NEB 10-beta E. coli</li> |
| − | <p class="main_name wikicolor ">Moreno Zolfo</p> | + | <li> K12 - JM109 E. coli</li> |
| | + | <li> BL21 - NEB Express E. coli</li> |
| | + | </ul> |
| | + | </section> |
| | + | </div> |
| | + | </section> |
| | + | |
| | + | |
| | + | <section class="wrapper style4 container" style="margin-top:1em;"> |
| | + | |
| | + | <!-- Content --> |
| | + | <div class="content wow fadeInLeft"> |
| | + | <section> |
| | + | <header> |
| | + | <h3>Personal Protective Equipment (PPE)</h3> |
| | + | </header> |
| | | | |
| − | <p> I am Moreno, a 23 years old Master Student at the University of Trento. I am currently attending a Degree Program in Cellular and Molecular Biotechnology after a B.Sc. in Computer Science in 2014 and have a strong interest in Computational Biology, Bioinformatics and Microbiology. In the iGEM Team I mainly care of the informatic services and the wiki-building. I love reading and photography and I am passionate into asian languages and cultures.</p> | + | <p> Last but non least we adopted the appropriate PPE during the lab work. We always used lab coats, nitrile gloves and goggles. We safely operated with biological material under the laminar flow cabinet BioAir SAFEMATE Series Class II 1. 8.</p> |
| − |
| + | <p> We used chemicals that are associated with hazard (ethidium bromide, azide, organic solvents) under the chemical hood Fume Hood MOD. ASEM® 120EN New - Class 0.</ p> |
| − | <p class="skill_list wikicolor"><span>Skills:</span> | + | <p>We carefully revised all the Material Safety Datasheet of each compound that was used prior to start an experiment.</p> |
| − | <i class="fli flaticon-cell8"></i> <span> Computer Expert</span>
| + | </section> |
| − | <i class="fli flaticon-businessman233"></i> <span> Geek in shirt</span>
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