Difference between revisions of "Team:San Andres/Parts"

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</table>
 
</table>
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  <div class="gwd-div-7xzo gwd-a-1thu">
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    <h1>Parts&nbsp;</h1>
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<h1>Parts&nbsp;</h1>
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    <big>Throughout the project we started to learn the fundamental
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<div style="text-align: justify;"><big>Throughout
 +
the project we started to learn the fundamental
 
principles of synthetic biology to get to work on our plasmid with
 
principles of synthetic biology to get to work on our plasmid with
 
which we want to see gluten degradation via the enzyme Kumamax. For
 
which we want to see gluten degradation via the enzyme Kumamax. For
 
this we going to insert in an e. coli the parts (Biobricks) needed to
 
this we going to insert in an e. coli the parts (Biobricks) needed to
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make our future bacteria can degrade gluten. The parts are:<br>
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make our future bacteria can degrade gluten. The parts are:</big><br>
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</big>
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<big></big></div>
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    <ul>
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<ul>
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      <li><big><span style="font-family: 'Arial','sans-serif';"><strong style="font-weight: bold;">Promoter
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  <li style="text-align: justify;"><big><span
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     <a href="http://parts.igem.org/Part:BBa_J23119">(BBa_J23119</a></strong><a href="http://parts.igem.org/Part:BBa_J23119"><span style="font-weight: bold;">)</span></a>:
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style="font-family: 'Arial','sans-serif';"><strong
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style="font-weight: bold;">Promoter
 +
     <a href="http://parts.igem.org/Part:BBa_J23119">(BBa_J23119</a></strong><a
 +
href="http://parts.igem.org/Part:BBa_J23119"><span
 +
style="font-weight: bold;">)</span></a>:
 
     </span>Constitutive
 
     </span>Constitutive
 
promoter (which works permanently) that is give in the relative
 
promoter (which works permanently) that is give in the relative
 
fluorescence of these plasmids in the TG1 strain grown in LB medium.</big>
 
fluorescence of these plasmids in the TG1 strain grown in LB medium.</big>
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      </li>
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  </li>
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      <li><big><strong style="font-weight: bold;">RBS</strong><strong style="font-weight: bold;">
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  <li style="text-align: justify;"><big><strong
 +
style="font-weight: bold;">RBS</strong><strong
 +
style="font-weight: bold;">
 
     <a href="http://parts.igem.org/Part:BBa_K1084103">(BBa_K1084103)</a></strong>:
 
     <a href="http://parts.igem.org/Part:BBa_K1084103">(BBa_K1084103)</a></strong>:
 
Synthetic RBS with uplifting sequence</big>.</li>
 
Synthetic RBS with uplifting sequence</big>.</li>
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      <li><big><strong>Vector:<span style="font-weight: normal;"> <a href="http://parts.igem.org/Part:pSB1C3">pSB1C3</a></span></strong></big>
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  <li style="text-align: justify;"><big><strong>Vector:<span
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      </li>
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style="font-weight: normal;"> <a
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      <li><big><strong><span style="font-family: 'Arial','sans-serif';">Coding Region:
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href="http://parts.igem.org/Part:pSB1C3">pSB1C3</a></span></strong></big>
 +
  </li>
 +
  <li style="text-align: justify;"><big><strong><span
 +
style="font-family: 'Arial','sans-serif';">Coding Region:
 
KumaMax <a href="http://parts.igem.org/Part:BBa_K590087">(BBa_K590087)</a>:</span></strong>
 
KumaMax <a href="http://parts.igem.org/Part:BBa_K590087">(BBa_K590087)</a>:</span></strong>
 
It
 
It
 
degrades gluten,
 
degrades gluten,
 
celiac disease leading cause. Enzyme generated by rational mutation for
 
celiac disease leading cause. Enzyme generated by rational mutation for
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the active site of it. It was created by the team IGEM <a href="https://2011.igem.org/Team:Washington/Celiacs/Background">Washington
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the active site of it. It was created by the team IGEM <a
 +
href="https://2011.igem.org/Team:Washington/Celiacs/Background">Washington
 
2011.</a></big>
 
2011.</a></big>
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      </li>
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  </li>
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      <li><big><strong><span style="font-family: 'Arial','sans-serif';">Reporter: RFP
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  <li style="text-align: justify;"><big><strong><span
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     <a href="http://parts.igem.org/Part:BBa_J04450">(BBa_J04450)</a>:</span></strong><span style="font-family: Arial,sans-serif;"> Red f</span>luorescence
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style="font-family: 'Arial','sans-serif';">Reporter: RFP
 +
     <a href="http://parts.igem.org/Part:BBa_J04450">(BBa_J04450)</a>:</span></strong><span
 +
style="font-family: Arial,sans-serif;"> Red f</span>luorescence
 
protein.</big>
 
protein.</big>
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      </li>
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  </li>
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      <li><big><span style="font-family: Arial,sans-serif;"><strong>Terminator
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  <li style="text-align: justify;"><big><span
 +
style="font-family: Arial,sans-serif;"><strong>Terminator
 
     <a href="http://parts.igem.org/Part:BBa_B0015">(BBa_B0015)</a>:</strong>
 
     <a href="http://parts.igem.org/Part:BBa_B0015">(BBa_B0015)</a>:</strong>
 
     </span>Dual terminator consisting of
 
     </span>Dual terminator consisting of
 
the B0010 and B0012 parties. It serves to give greater efficiency in
 
the B0010 and B0012 parties. It serves to give greater efficiency in
 
transcription</big>.</li>
 
transcription</big>.</li>
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    </ul>
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</ul>
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    <div style="text-align: center;">
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<div style="text-align: center;">
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      <img alt="File:Parts.jpg" src="https://static.igem.org/mediawiki/2015/e/e8/Parts.jpg" height="59" width="288">
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<img alt="File:Parts.jpg"
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      <br>
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src="https://static.igem.org/mediawiki/2015/e/e8/Parts.jpg"
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      <img alt="File:Circuito.jpg" src="https://static.igem.org/mediawiki/2015/thumb/2/2a/Circuito.jpg/800px-Circuito.jpg" height="309" width="800">
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height="59" width="288"><br>
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      <br>
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<img alt="File:Circuito.jpg"
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      <div style="text-align: left;"><big>This is a
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src="https://static.igem.org/mediawiki/2015/thumb/2/2a/Circuito.jpg/800px-Circuito.jpg"
 +
height="309" width="800">
 +
<br>
 +
<div style="text-align: left;">
 +
<div style="text-align: justify;"><big>This is a
 
graphic model of as it has be our plasmid where we can visualize the
 
graphic model of as it has be our plasmid where we can visualize the
 
promoter, the RBS, the enzyme KumaMax, the RFP and the terminator,
 
promoter, the RBS, the enzyme KumaMax, the RFP and the terminator,
 
joined by means of prefixes and suffixes that indicate the locations of
 
joined by means of prefixes and suffixes that indicate the locations of
 
court.</big>
 
court.</big>
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        <br>
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<br>
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        <br>
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</div>
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        <div style="text-align: center;">
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<br>
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          <img alt="File:Plasmido.jpg" src="https://static.igem.org/mediawiki/2015/thumb/c/ce/Plasmido.jpg/664px-Plasmido.jpg" height="600" width="664">
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<div style="text-align: center;">
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        </div>
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<img alt="File:Plasmido.jpg"
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      </div>
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src="https://static.igem.org/mediawiki/2015/thumb/c/ce/Plasmido.jpg/664px-Plasmido.jpg"
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    </div>
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height="600" width="664"></div>
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    <big><br>
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</div>
 +
</div>
 +
<big><br>
 
</big>
 
</big>
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  </div>
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</div>
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  <br>
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<br>
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  <a href="https://2015.igem.org/Main_Page" class="gwd-a-3xpx gwd-a-36ww">
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<a href="https://2015.igem.org/Main_Page"
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    <img style="border: 0px solid ; width: 150px; height: 150px; top: 35px; left: 989px;" alt="" src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" class="gwd-img-kwqf">
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class="gwd-a-3xpx gwd-a-36ww">
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  </a>
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<img
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  <a href="https://igem.org/Team_List?year=2015" class="gwd-a-3xpx">
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style="border: 0px solid ; width: 150px; height: 150px; top: 35px; left: 989px;"
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    <img src="https://static.igem.org/mediawiki/2015/thumb/9/94/Logo43.png/454px-Logo43.png" class="gwd-img-l2p5">
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alt="" src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"
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  </a>
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class="gwd-img-kwqf"></a>
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  <br>
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<a href="https://igem.org/Team_List?year=2015"
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  <br>
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class="gwd-a-3xpx"><img
 +
src="https://static.igem.org/mediawiki/2015/thumb/9/94/Logo43.png/454px-Logo43.png"
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class="gwd-img-l2p5">
 +
</a><br>
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<br>
 
</body>
 
</body>
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</html>
 
</html>

Revision as of 23:26, 19 August 2015

wiki exp wiki wiki wiki 2   File:Gluten-s-Job.jpeg

Home Team Project Parts Modeling
Results Notebook Human Practices Future Projections Collaborations

Parts 

Throughout the project we started to learn the fundamental principles of synthetic biology to get to work on our plasmid with which we want to see gluten degradation via the enzyme Kumamax. For this we going to insert in an e. coli the parts (Biobricks) needed to make our future bacteria can degrade gluten. The parts are:
  • Promoter (BBa_J23119): Constitutive promoter (which works permanently) that is give in the relative fluorescence of these plasmids in the TG1 strain grown in LB medium.
  • RBS (BBa_K1084103): Synthetic RBS with uplifting sequence.
  • Vector: pSB1C3
  • Coding Region: KumaMax (BBa_K590087): It degrades gluten, celiac disease leading cause. Enzyme generated by rational mutation for the active site of it. It was created by the team IGEM Washington 2011.
  • Reporter: RFP (BBa_J04450): Red fluorescence protein.
  • Terminator (BBa_B0015): Dual terminator consisting of the B0010 and B0012 parties. It serves to give greater efficiency in transcription.
File:Parts.jpg
File:Circuito.jpg
This is a graphic model of as it has be our plasmid where we can visualize the promoter, the RBS, the enzyme KumaMax, the RFP and the terminator, joined by means of prefixes and suffixes that indicate the locations of court.

File:Plasmido.jpg