Difference between revisions of "Team:ETH Zurich/Modeling/Lactate Module"
| Line 12: | Line 12: | ||
<tr> <th>Name </th> <th>Description </th> </tr> | <tr> <th>Name </th> <th>Description </th> </tr> | ||
<tr> <td>Lac<SUB>out</SUB></td> <td> Lactate produced by mammalian cells </td> </tr> | <tr> <td>Lac<SUB>out</SUB></td> <td> Lactate produced by mammalian cells </td> </tr> | ||
| − | <tr> <td>Lac<SUB>in</SUB> </td> <td>Lactate inside | + | <tr> <td>Lac<SUB>in</SUB> </td> <td>Lactate inside <i>E. coli </i> cells </td> </tr> |
<tr> <td>L<SUB>2</SUB> </td> <td>Lldr, regulatory protein of lld operon, acts as a repressor</td> </tr> | <tr> <td>L<SUB>2</SUB> </td> <td>Lldr, regulatory protein of lld operon, acts as a repressor</td> </tr> | ||
<tr> <td>LacI</td> <td>Lac repressor, DNA-binding protein, acts as a protein</td> </tr> | <tr> <td>LacI</td> <td>Lac repressor, DNA-binding protein, acts as a protein</td> </tr> | ||
Revision as of 13:15, 20 August 2015
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Lactate Module
Introduction
The initial idea was to implement a fold change sensor in order to measure the lactate production rate. We assumed that on our timeframe, the lactate production might not reach steady state. That is why our sensor has the topology of a fold change sensor. However due to the topology of the natural detection system in E coli. Our system does not behave as a fold change sensor but rather amplifies the difference between the production of cancer and normal cells.
Chemical species
| Name | Description |
|---|---|
| Lacout | Lactate produced by mammalian cells |
| Lacin | Lactate inside E. coli cells |
| L2 | Lldr, regulatory protein of lld operon, acts as a repressor |
| LacI | Lac repressor, DNA-binding protein, acts as a protein |
| IPTG | Isopropyl β-D-1-thiogalactopyranoside, prevents LacI from repressing the gene of interest |