Difference between revisions of "Team:ETH Zurich/Modeling/Parameters"

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<tr><td>\(\text{LuxR}_\text{tot}\)</td><td>0.0025 &mu;M</td><td>Total concentration of LuxR </td><td>assumed</td></tr>
 
<tr><td>\(\text{LuxR}_\text{tot}\)</td><td>0.0025 &mu;M</td><td>Total concentration of LuxR </td><td>assumed</td></tr>
 
<tr><td>\(a_\mathrm{LuxI}\)</td><td>1 &mu;M.min<SUP>-1</SUP></td><td>Maximal production rate of LuxI</td><td><a href="https://2015.igem.org/Team:ETH_Zurich/References#Basu2005">Basu, 2005</a></td></tr>
 
<tr><td>\(a_\mathrm{LuxI}\)</td><td>1 &mu;M.min<SUP>-1</SUP></td><td>Maximal production rate of LuxI</td><td><a href="https://2015.igem.org/Team:ETH_Zurich/References#Basu2005">Basu, 2005</a></td></tr>
 +
<tr><td>\(k_\mathrm{leaky}\)</td><td>0.0005 &mu;M<SUP>-1</SUP></td><td>Leakiness of P<SUB>LuxR</SUB> promoter </td><td>assumed</td></tr>
 +
<tr><td>\(K_\mathrm{a,LuxRAHL}\)</td><td>0.01 &mu;M</td><td>Activation coefficient of LuxRAHL </td><td>assumed</td></tr>
 +
<tr><td>\(d_\mathrm{LuxI}\)</td><td>0.0167 min<SUP>-1</SUP></td><td>Degradation rate of LuxI </td><td>assumed</td></tr>
 +
<tr><td>\a_\mathrm{AHL}\)</td><td>0.04 &mu;M.min<SUP>-1</SUP></td><td>Production rate of AHL </td><td>assumed</td></tr>
 
</table>
 
</table>
  

Revision as of 15:15, 20 August 2015

"What I cannot create I do not understand."
- Richard Feynmann

Parameters

AHL module

Single cell model

Name Description Value References/Estimation
\(K_{d,\text{LuxRAHL}}\)100 nMDissociation constant between luxR and AHLWeber, 2013
\(\text{LuxR}_\text{tot}\)0.0025 μMTotal concentration of LuxR assumed
\(a_\mathrm{LuxI}\)1 μM.min-1Maximal production rate of LuxIBasu, 2005
\(k_\mathrm{leaky}\)0.0005 μM-1Leakiness of PLuxR promoter assumed
\(K_\mathrm{a,LuxRAHL}\)0.01 μMActivation coefficient of LuxRAHL assumed
\(d_\mathrm{LuxI}\)0.0167 min-1Degradation rate of LuxI assumed
\a_\mathrm{AHL}\)0.04 μM.min-1Production rate of AHL assumed

Lactate module

Single cell model

Assumptions

Name Description Value References/Estimation
\(\text{Lac}_{\text{ini}}\) Initial concentration of lactate in the medium 1 μM Low concentration of lactate in the medium
Kdllini Initial concentration of lactate in the medium