Difference between revisions of "Team:ETH Zurich/Modeling/Parameters"
Line 10: | Line 10: | ||
<tr><td>\(\text{LuxR}_\text{tot}\)</td><td>0.0025 μM</td><td>Total concentration of LuxR </td><td>assumed</td></tr> | <tr><td>\(\text{LuxR}_\text{tot}\)</td><td>0.0025 μM</td><td>Total concentration of LuxR </td><td>assumed</td></tr> | ||
<tr><td>\(a_\mathrm{LuxI}\)</td><td>1 μM.min<SUP>-1</SUP></td><td>Maximal production rate of LuxI</td><td><a href="https://2015.igem.org/Team:ETH_Zurich/References#Basu2005">Basu, 2005</a></td></tr> | <tr><td>\(a_\mathrm{LuxI}\)</td><td>1 μM.min<SUP>-1</SUP></td><td>Maximal production rate of LuxI</td><td><a href="https://2015.igem.org/Team:ETH_Zurich/References#Basu2005">Basu, 2005</a></td></tr> | ||
+ | <tr><td>\(k_\mathrm{leaky}\)</td><td>0.0005 μM<SUP>-1</SUP></td><td>Leakiness of P<SUB>LuxR</SUB> promoter </td><td>assumed</td></tr> | ||
+ | <tr><td>\(K_\mathrm{a,LuxRAHL}\)</td><td>0.01 μM</td><td>Activation coefficient of LuxRAHL </td><td>assumed</td></tr> | ||
+ | <tr><td>\(d_\mathrm{LuxI}\)</td><td>0.0167 min<SUP>-1</SUP></td><td>Degradation rate of LuxI </td><td>assumed</td></tr> | ||
+ | <tr><td>\a_\mathrm{AHL}\)</td><td>0.04 μM.min<SUP>-1</SUP></td><td>Production rate of AHL </td><td>assumed</td></tr> | ||
</table> | </table> | ||
Revision as of 15:15, 20 August 2015
- Project
- Modeling
- Lab
- Human
Practices - Parts
- About Us
Parameters
AHL module
Single cell model
Name | Description | Value | References/Estimation |
---|---|---|---|
\(K_{d,\text{LuxRAHL}}\) | 100 nM | Dissociation constant between luxR and AHL | Weber, 2013 |
\(\text{LuxR}_\text{tot}\) | 0.0025 μM | Total concentration of LuxR | assumed |
\(a_\mathrm{LuxI}\) | 1 μM.min-1 | Maximal production rate of LuxI | Basu, 2005 |
\(k_\mathrm{leaky}\) | 0.0005 μM-1 | Leakiness of PLuxR promoter | assumed |
\(K_\mathrm{a,LuxRAHL}\) | 0.01 μM | Activation coefficient of LuxRAHL | assumed |
\(d_\mathrm{LuxI}\) | 0.0167 min-1 | Degradation rate of LuxI | assumed |
\a_\mathrm{AHL}\) | 0.04 μM.min-1 | Production rate of AHL | assumed |
Lactate module
Single cell model
Assumptions
Name | Description | Value | References/Estimation |
---|---|---|---|
\(\text{Lac}_{\text{ini}}\) | Initial concentration of lactate in the medium | 1 μM | Low concentration of lactate in the medium |
Kdllini | Initial concentration of lactate in the medium |