Difference between revisions of "Team:Manchester-Graz/Project/Vectordesign"
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To avoid any leaky read through of transcription terminators, the constitutively expressed transcripts of the regulatory proteins of the two QS-systems as well as the beta-lactamase resistance marker, are positioned in the opposite direction of the auto-induced P<sub>aidA</sub> – and P<sub>esaRC</sub> –promoter (Figure 3). <br>Additionally P<sub>aidA</sub> is placed upfront of P<sub>esaRC</sub>. All reporter genes can easily be replaced by any other genes by standard cloning techniques. <br></p></p> | To avoid any leaky read through of transcription terminators, the constitutively expressed transcripts of the regulatory proteins of the two QS-systems as well as the beta-lactamase resistance marker, are positioned in the opposite direction of the auto-induced P<sub>aidA</sub> – and P<sub>esaRC</sub> –promoter (Figure 3). <br>Additionally P<sub>aidA</sub> is placed upfront of P<sub>esaRC</sub>. All reporter genes can easily be replaced by any other genes by standard cloning techniques. <br></p></p> | ||
− | <p>We want to assemble two variants of pCERI that only slightly differ in the positioning of the regulatory proteins EsaR and CepR on the transcript of P<sub>esaS</sub>. The first variant will be assembled according to Figure 3, while in an alternative version (v2) the genes encoding esaR and cepR are switched. Usually genes further downstream on the transcript get translated less efficiently. Thus the switch in the positioning of the regulatory proteins on the transcript might influence the expression behavior of our regulatory system </p> | + | <p>We want to assemble two variants of pCERI that only slightly differ in the positioning of the regulatory proteins EsaR and CepR on the transcript of P<sub>esaS</sub>. The first variant will be assembled according to Figure 3, while in an alternative version (v2) the genes encoding esaR and cepR are switched. Usually, genes further downstream on the transcript get translated less efficiently. Thus, the switch in the positioning of the regulatory proteins on the transcript might influence the expression behavior of our regulatory system </p> |
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Revision as of 08:00, 27 August 2015
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