Difference between revisions of "Team:Gifu/InterLab"

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Setting of plate reader is following:<br>
 
Setting of plate reader is following:<br>
 
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<font size="6" face="Arimo">Result and discussion</font>
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<li> <font size="5" face="Arimo"> Definition<br><br></font> </li>
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We expressed absorbance as relative value for　positive control. This expression is following:<br>
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Revision as of 07:09, 28 August 2015


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Inter Lab Study


Equipment

  • BioSHAKER TA-12R
    • use for shaking culture

  • Safire ART-NR:F129013
    • use for measurement of absorbance


Method of Measurement
      We constructed 5 plasmids [positive control, negative control, device1~3] by using BioBricks and then transformed to E. coli K-12 JM109. We checked them by colony direct PCR and plasmids sequence. We cultivated correct bacterias by shaking until OD660:0.5. Cultivated mediums were　transferred to 96-well plate by 100mL. We read the absorbance.

    Setting of plate reader is following:


Result and discussion

  • Definition
    • We expressed absorbance as relative value for　positive control. This expression is following:




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