Difference between revisions of "Team:Gifu/InterLab"

Inter Lab Study

Equipment

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• BioSHAKER TA-12R
  
use for shaking culture




• Safire ART-NR:F129013
  
use for measurement of absorbance

Method of Measurement

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  We constructed 5 plasmids [positive control, negative control, device1~3] by using BioBricks and then transformed to E. coli K-12 JM109. We checked them by colony direct PCR and plasmids sequence. We cultivated correct bacterias by shaking until OD660:0.5. Cultivated mediums were　transferred to 96-well plate by 100mL. We read the absorbance.

Setting of plate reader is following:

Result and discussion

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• Definition
  
We expressed absorbance as relative value for　positive control. This expression is following:




• Value
  
Relative absorbance values for positive control are shown below.