Difference between revisions of "Team:Gifu/InterLab"

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&nbsp;&nbsp;We constructed 5 plasmids [positive control, negative control, device1~3] by using BioBricks and then transformed to <i>E. coli</i> K-12 JM109. We checked them by colony direct PCR and plasmids sequence. We cultivated correct bacterias by shaking until OD660:0.5. Cultivated mediums were　transferred to 96-well plate by 100mL. We read the absorbance.
 
&nbsp;&nbsp;We constructed 5 plasmids [positive control, negative control, device1~3] by using BioBricks and then transformed to <i>E. coli</i> K-12 JM109. We checked them by colony direct PCR and plasmids sequence. We cultivated correct bacterias by shaking until OD660:0.5. Cultivated mediums were　transferred to 96-well plate by 100mL. We read the absorbance.
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Setting of plate reader is following:<br>
 
Setting of plate reader is following:<br>
 
<img src="https://static.igem.org/mediawiki/2015/2/20/Set_safari_gify.png" border="0" width="395" height="220">
 
<img src="https://static.igem.org/mediawiki/2015/2/20/Set_safari_gify.png" border="0" width="395" height="220">

Revision as of 07:17, 28 August 2015


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Inter Lab Study


Equipment

  • BioSHAKER TA-12R
    • use for shaking culture



  • Safire ART-NR:F129013
    • use for measurement of absorbance




Method of Measurement
      We constructed 5 plasmids [positive control, negative control, device1~3] by using BioBricks and then transformed to E. coli K-12 JM109. We checked them by colony direct PCR and plasmids sequence. We cultivated correct bacterias by shaking until OD660:0.5. Cultivated mediums were　transferred to 96-well plate by 100mL. We read the absorbance.
    Setting of plate reader is following:




Result and discussion

  • Definition
    • We expressed absorbance as relative value for　positive control. This expression is following:



  • Value
    • Relative absorbance values for positive control are shown below.











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