Revision as of 13:05, 28 August 2015

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

Bio LOGIC

Logic Orthogonal gRNA Implemented Circuits

Engineering transcriptional logic gates to program cellular behavior remains an important challenge for synthetic biology. Currently, genetic circuits reproducing digital logic are limited in scalability and robustness by output discrepancies and crosstalk between transcriptional pathways. We propose to address these issues using RNA-guided dCas9 fused to a transcription activation domain as a programmable transcription factor.

Project

Background

Discover the ideas behind our project, from boolean logic to the the complete explanation of a biologic logic gate.

Description

Understand our project in the smallest details and explore the world of CRISPR-dCas9 with us.

Applications

Need a biosensor? Want a bacteria that can depollute a lake? Here are a few examples of how you could apply our technology for your own designs.

Modeling

Having a robust system is the key when devising new concepts. It enables testing in silico before ordering the expensive parts...

Achievements

In Vivo

Our achievements in the wet-lab, the result of a summer in the cold labrooms.

In silico

What we were able to do using the computer.

Judging

The criterias that we fulfilled for the different medals.

Parts

iGEM is all about Biobricks. Here is our contribution to this expanding library.

Policy and Practices

Ethics

Is working on the genome ethically correct? We tried to answer to this question.

Safety

Doing safe work with small organisms can be dangerous. Here is what we did to protect ourselves and the environment.

Human Practices

We do good ! Don't listen to the mass media.

Notebook

E. coli

See how we planned our summer and how it went for the E.coli team.

Yeast

Even if we used a beer icon Yeasts are not all about beer, discover how we experimented with this tough organism.

Protocols

These are the protocols that you need if you want to redo our work.

Team

Meet us!

Brief description of who is who and for what (s)he is renowned.

Timeline

How did our summer go?

Aknowledgements

We are thankfull to all the people that helped us.

Attributions

What you do is what you get.

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

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