Difference between revisions of "Team:Gifu/Safety"

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<li><a href="https://2015.igem.org/Team:Gifu/InterLab" style="text-decoration:none;" style="text-decoration:none;" >InterLab Study&nbsp;&nbsp;&nbsp;</a></li>
 
<li><a href="https://2015.igem.org/Team:Gifu/InterLab" style="text-decoration:none;" style="text-decoration:none;" >InterLab Study&nbsp;&nbsp;&nbsp;</a></li>
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<li><a href="https://2015.igem.org/Team:Gifu/Safety"style="text-decoration:none;" > SAFETY&nbsp; </a></li>
 
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Revision as of 07:08, 29 August 2015


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Inter Lab Study


Equipment

  • BioSHAKER TA-12R
    • use for shaking culture



  • Safire ART-NR:F129013
    • use for measurement of absorbance




Method of Measurement
      We constructed 5 plasmids [positive control, negative control, device1~3] by using BioBricks and then transformed to E. coli K-12 JM109. We checked them by colony direct PCR and plasmids sequence. We cultivated correct bacterias by shaking until OD660:0.5. Cultivated mediums were　transferred to 96-well plate by 100mL. We read the absorbance.
      Setting of plate reader is following:



Result and discussion

  • Definition
    • We expressed absorbance as relative value for　positive control.
    This expression is following:
      Sample : Absorbance in sample
      NCav : Average of absorbance in negative control
      PCav : Average of absorbance in positive control



  • Value
    • Relative absorbance values for positive control are shown below.











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