Difference between revisions of "Team:HSNU-TAIPEI/Project/Benzo a pyrene"
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<p><font face="Comic Sans MS"> Let it produce itself.</font></p> | <p><font face="Comic Sans MS"> Let it produce itself.</font></p> | ||
<img src="https://static.igem.org/mediawiki/2015/e/ed/2015hsnu-benzo-a-pyrene_2.png" height="350" width="500"> | <img src="https://static.igem.org/mediawiki/2015/e/ed/2015hsnu-benzo-a-pyrene_2.png" height="350" width="500"> | ||
− | <p><font face="Comic Sans MS"> | + | <p><font face="Comic Sans MS"> QsrR is a protein which can combine with Bap-1,6-quinone.[5]As Laccase,we put the</font></p> |
− | <p><font face="Comic Sans MS"> | + | <p><font face="Comic Sans MS"> gene fragment in the E.coli.Let it produce itself.</font></p> |
− | <p><font face="Comic Sans MS"> | + | <img src="https://static.igem.org/mediawiki/2015/7/7d/2015hsnu-benzo-a-pyrene_3.png" height="300" width="550"> |
− | <img src="https://static.igem.org/mediawiki/2015/ | + | <p><font face="Comic Sans MS"> We design a gene part make QsrR repress sfRFP’s production.</font></p> |
+ | <p><font face="Comic Sans MS"> Usually,QsrR is on the Binding Site,and red fluorescent protein will not be produced.</font></p> | ||
+ | <p><font face="Comic Sans MS"> When QsrR combine with Bap-1,6-quinone, QsrR will be activated and go away.</font></p> | ||
+ | <p><font face="Comic Sans MS"> And the transcription will carry on,then it will produce red fluorescent protein</font></p> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/8/8e/2015hsnu-benzo-a-pyrene_4.png" height="400" width="550"> | ||
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<h2><font color="#008b8b"> ✡ Reference</font></h2> | <h2><font color="#008b8b"> ✡ Reference</font></h2> | ||
− | <p><font face="Comic Sans MS"> ( | + | <p><font face="Comic Sans MS"> [1] 黄靖芬,李来好,陈胜军,杨贤庆..烟熏食品中苯并(a)芘的产生机理及防止方法smoked foods Mechanism of benzo (a) pyrene and prevention methods</font></p> |
− | <p><font face="Comic Sans MS"> ( | + | <p><font face="Comic Sans MS"> [2] 降低食品中多環芳香族碳氫化合物含量之作業指引(草案) reduce operating guidelines in food polycyclic aromatic hydrocarbon content of the (draft)</font></p> |
− | <p><font face="Comic Sans MS"> ( | + | <p><font face="Comic Sans MS"> [3] Basic Information about Benzo(a)pyrene in Drinking Water</font></p> |
− | <p><font face="Comic Sans MS"> | + | <p><font face="Comic Sans MS"> [4] Hadibarata T, Kristanti RA. Identification of metabolites from benzo[a]pyrene oxidation by ligninolytic enzymes of Polyporus sp. S133. J Environ Manage. 2012 11/30;111(0):115-9.(2013 IGEM CUHK)</font></p> |
+ | <p><font face="Comic Sans MS"> [5] Ji Q, Zhang L, Jones MB, Sun F, Deng X, Liang H, et al. Molecular mechanism of quinone signaling mediated through S-quinonization of a YodB family repressor QsrR. Proceedings of the National </font></p> | ||
+ | <p><font face="Comic Sans MS"> Academy of Sciences. 2013 March 26;110(13):5010-5. (2013 IGEM CUHK)</font></p> | ||
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Latest revision as of 12:12, 30 August 2015
Benzo[a]pyrene
✡ Introduction
(1)Why do we detect benzo[a]pyrene?
Because when food,such as fried food ,which is heated above 300 degrees
celcius,food will release benzo[a]pyrene recycled oil usually contain
benzo[a]pyrene[1].
(2)Harm?
Pathway:from skin,breathing in,eating Carcinogenic,environmental
pollution, Skin irritation,eye irritation[1].
(3)Taiwanese regulation
[2](4)National regulation
Europe:The same to Taiwanese regulation[2]
American:The MCL has been set at 0.2 ppb[3]
✡ Circuit Design
So far,we don’t find the protein which can combine with the Benzo[a]pyrene,so first
we must degradate it by Laccase. And its product is Bap-1,6-quinone which can
combine with the protein,in order that we can detect Benzo[a]pyrene. [4]
We put the gene fragment which can produce the Laccase in the E.coli
Let it produce itself.
QsrR is a protein which can combine with Bap-1,6-quinone.[5]As Laccase,we put the
gene fragment in the E.coli.Let it produce itself.
We design a gene part make QsrR repress sfRFP’s production.
Usually,QsrR is on the Binding Site,and red fluorescent protein will not be produced.
When QsrR combine with Bap-1,6-quinone, QsrR will be activated and go away.
And the transcription will carry on,then it will produce red fluorescent protein
✡ Result
✡ Reference
[1] 黄靖芬,李来好,陈胜军,杨贤庆..烟熏食品中苯并(a)芘的产生机理及防止方法smoked foods Mechanism of benzo (a) pyrene and prevention methods
[2] 降低食品中多環芳香族碳氫化合物含量之作業指引(草案) reduce operating guidelines in food polycyclic aromatic hydrocarbon content of the (draft)
[3] Basic Information about Benzo(a)pyrene in Drinking Water
[4] Hadibarata T, Kristanti RA. Identification of metabolites from benzo[a]pyrene oxidation by ligninolytic enzymes of Polyporus sp. S133. J Environ Manage. 2012 11/30;111(0):115-9.(2013 IGEM CUHK)
[5] Ji Q, Zhang L, Jones MB, Sun F, Deng X, Liang H, et al. Molecular mechanism of quinone signaling mediated through S-quinonization of a YodB family repressor QsrR. Proceedings of the National
Academy of Sciences. 2013 March 26;110(13):5010-5. (2013 IGEM CUHK)