Difference between revisions of "Team:Gifu/InterLab"

Revision as of 04:02, 31 August 2015

Inter Lab Study

Equipment

BioSHAKER TA-12R

use for shaking culture

Safire ART-NR:F129013

use for measurement of absorbance

Method of Measurement

  We constructed 5 plasmids [positive control, negative control, device1~3] by using BioBricks and then transformed to E. coli K-12 JM109. We checked them by colony direct PCR and plasmids sequence. We cultivated correct bacterias by shaking until OD660:0.5. Cultivated mediums were　transferred to 96-well plate by 100mL. We read the absorbance.

  Setting of plate reader is following:

Result

Definition

We expressed absorbance as relative value for　positive control.
This expression is following:

  Sample : Absorbance in sample
  NCav : Average of absorbance in negative control
  PCav : Average of absorbance in positive control

Value

Relative absorbance values for positive control are shown below.

FIG1 Relative absorbance values
20A : Device1 (J23101 + I13504)
22A : Device2 (J23106 + I13504)
22K : Device3 (J23117 + I13504)

Discussion

  The value of device1 is much higher than that of device2 and 3. This is why each device is different from promoter sequence, especially -10 and -35 region.

Promoter sequence in device1 resembles consensus sequence. On the other hand, device2 and 3 is not similar than device1. This is how each GFP expression is different.

MENU

@@ Line 100: / Line 100: @@
 <li> <font size="5" face="Arimo"> Definition<br></font> </li>
 <font size="4" face="Arimo">
-We expressed absorbance as relative value for　positive control. <br>This expression is following:<br></font>
+<p>We expressed absorbance as relative value for　positive control. <br>This expression is following:<br></font></p>
 <img src="https://static.igem.org/mediawiki/2015/8/85/Siki-gifu.png" border="0" width="211" height="57"><br>
 &nbsp;&nbsp;Sample : Absorbance in sample<br>
@@ Line 109: / Line 109: @@
 <li> <font size="5" face="Arimo"> Value<br></font> </li>
 <font size="4" face="Arimo">
-Relative absorbance values for positive control are shown below. <br>
+<p>Relative absorbance values for positive control are shown below. <br></p>
 <img src="https://static.igem.org/mediawiki/2015/e/e4/GURAHU-gifu.png" border="0" width="572" height="364"> <br><br>
                        FIG1 Relative absorbance values <br></font>
@@ Line 121: / Line 121: @@
 <ul>
 <font size="4" face="Arimo">
-&nbsp;&nbsp;The value of device1 is much higher than that of device2 and 3. This is why each device is different from promoter sequence, especially -10 and -35 region.<br><br>
+<p>&nbsp;&nbsp;The value of device1 is much higher than that of device2 and 3. This is why each device is different from promoter sequence, especially -10 and -35 region.<br><br></p>
 <img src="https://static.igem.org/mediawiki/2015/b/b4/Konnsennsasu2.png" border="0" width="413" height="105"> <br><br>
-Promoter sequence in device1 resembles consensus sequence. On the other hand, device2 and 3 is not similar than device1. This is how each GFP expression is different.
+<p>Promoter sequence in device1 resembles consensus sequence. On the other hand, device2 and 3 is not similar than device1. This is how each GFP expression is different.</p>
   <br><br><br>