Difference between revisions of "Team:EPF Lausanne/Timeline"

 
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   <ul class="timeline">
 
   <ul class="timeline">
 
     <li class="timeline-inverted">
 
     <li class="timeline-inverted">
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     <div class="timeline-badge"></div>
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     <div class="timeline-badge info"></div>
 +
    <div class="timeline-panel">
 +
      <div class="timeline-heading">
 +
        <h4 class="timeline-title">Our project has a name!</h4>
 +
        <h4 class="timeline-title">July 8th 2015</h4>
 +
      </div>
 +
      <div class="timeline-body">
 +
        <p>After a few looongs discussions and many ideas we choose our name:</br><b>Bio LOGIC - Logic Orthogonal gRNA-Implemented Circuits</b></p>
 +
      </div>
 +
    </div>
 +
    </li>
 +
    <li>
 +
    <div class="timeline-badge info"></div>
 +
    <div class="timeline-panel">
 +
      <div class="timeline-heading">
 +
        <h4 class="timeline-title">Get back to work!</h4>
 +
        <h4 class="timeline-title">July 1st 2015</h4>
 +
      </div>
 +
      <div class="timeline-body">
 +
        <p>First of many meetings this summer! The day after our exams finished, we all got together to set some goals for the next few weeks. Let the fun begin!</p>
 +
      </div>
 +
    </div>
 +
    </li>
 +
    <li class="timeline-inverted">
 +
    <div class="timeline-badge info"></div>
 +
    <div class="timeline-panel">
 +
      <div class="timeline-heading">
 +
        <h4 class="timeline-title">Break time</h4>
 +
        <h4 class="timeline-title">June 1st - July 1st 2015</h4>
 +
      </div>
 +
      <div class="timeline-body">
 +
        <p>Our project was put on hold during our exams. :(</p>
 +
      </div>
 +
    </div>
 +
    </li>
 +
    <li>
 +
    <div class="timeline-badge warning"></div>
 +
    <div class="timeline-panel">
 +
      <div class="timeline-heading">
 +
        <h4 class="timeline-title">Working in the lab</h4>
 +
        <h4 class="timeline-title">April 21st - May 31st 2015</h4>
 +
      </div>
 +
      <div class="timeline-body">
 +
        <p>Lab work is slow because we have to follow all our courses at the same time. We are able to make our first construct pdCas9-w after mastering the art of PCR and Gibson assembly!</p>
 +
      </div>
 +
    </div>
 +
    </li>
 +
    <li class="timeline-inverted">
 +
    <div class="timeline-badge warning"></div>
 
     <div class="timeline-panel">
 
     <div class="timeline-panel">
 
       <div class="timeline-heading">
 
       <div class="timeline-heading">
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         <h4 class="timeline-title">The bible</h4>
+
         <h4 class="timeline-title">Presents!</h4>
 +
        <h4 class="timeline-title">April 14th 2015</h4>
 
       </div>
 
       </div>
 
       <div class="timeline-body">
 
       <div class="timeline-body">
−
         <p>The E. Coli part of our project is strongly based on the paper of Bikard et al. "Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system". Bikard sent us his strains JEN202, JEN202+PWJ89 and DH5A+PWJ66. We also ordered a plasmid containing dCas9 and the synthetic DNA encoding fou our gRNAs.</p>
+
         <p>The E. Coli part of our project is strongly based on the paper of Bikard et al. "Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system". Bikard sent us his strains JEN202, JEN202+PWJ89 and DH5A+PWJ66. What a generous guy!</p>
 
       </div>
 
       </div>
 
     </div>
 
     </div>
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       <div class="timeline-heading">
 
       <div class="timeline-heading">
 
         <h4 class="timeline-title">Blast</h4>
 
         <h4 class="timeline-title">Blast</h4>
−
         <h4 class="timeline-title">02.04.2015</h4>
+
         <h4 class="timeline-title">April 2nd 2015</h4>
 
       </div>
 
       </div>
 
       <div class="timeline-body">
 
       <div class="timeline-body">
−
         <p>In our project dCas9 target DNA sequences using gRNAs and acts as an activator or an inhibitor depending on its position. Axel and Cyril created a C++ program to blast random generated  gRNA sequences on the E. Coli or Yeast genome.</p>
+
         <p>In our project dCas9-w targets DNA sequences using gRNAs and acts as an activator or an inhibitor depending on its position. Axel and Cyril created a C++ program to blast random generated  gRNA sequences on the E. Coli or Yeast genome.</p>
 
       </div>
 
       </div>
 
     </div>
 
     </div>
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     <div class="timeline-panel">
 
     <div class="timeline-panel">
 
       <div class="timeline-heading">
 
       <div class="timeline-heading">
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         <h4 class="timeline-title">And the winner is...</h4>
+
         <h4 class="timeline-title">And our project is...</h4>
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         <h4 class="timeline-title">26.03.2015</h4>
+
         <h4 class="timeline-title">March 26th 2015</h4>
 
       </div>
 
       </div>
 
       <div class="timeline-body">
 
       <div class="timeline-body">
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         <p>...the new framework for biologic circuits using dCas9.</p>
+
         <p>...the new framework for biologic circuits using dCas9-w as a synthetic transcription factor. Go to our <a href="https://2015.igem.org/Team:EPF_Lausanne/Project/Description">project description</a>page to find out more!</p>
 
         <img src="https://static.igem.org/mediawiki/2015/9/96/Ari_dCas9.jpg" width="100%">
 
         <img src="https://static.igem.org/mediawiki/2015/9/96/Ari_dCas9.jpg" width="100%">
 
       </div>
 
       </div>
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       <div class="timeline-heading">
 
       <div class="timeline-heading">
 
         <h4 class="timeline-title">The lab starts!</h4>
 
         <h4 class="timeline-title">The lab starts!</h4>
−
         <h4 class="timeline-title">24.03.2015 - 26.03.2015</h4>
+
         <h4 class="timeline-title">March 24th -31st 2015</h4>
 
       </div>
 
       </div>
 
       <div class="timeline-body">
 
       <div class="timeline-body">
−
         <p>TAs start to train us in the lab: medium and agar plates preparation</p>
+
         <p>Advisors start to train us in the lab: LB medium and LB agar plates preparation, bacterial cultures, minipreps, PCR, etc.</p>
 
       </div>
 
       </div>
 
     </div>
 
     </div>
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       <div class="timeline-heading">
 
       <div class="timeline-heading">
 
         <h4 class="timeline-title">Brainstorming finalists</h4>
 
         <h4 class="timeline-title">Brainstorming finalists</h4>
−
         <h4 class="timeline-title">19.03.2015</h4>
+
         <h4 class="timeline-title">March 19th 2015</h4>
 
       </div>
 
       </div>
 
       <div class="timeline-body">
 
       <div class="timeline-body">
 
         <p>Three cool ideas came out of the brainstorming:  
 
         <p>Three cool ideas came out of the brainstorming:  
 
           <ul>
 
           <ul>
−
             <li><p>A new framework for biologic circuits </p></li>
+
             <li><p>A new framework for biologic circuits</p></li>
−
             <li><p>Lung cancer therapy using dCas9 </p></li>
+
             <li><p>Lung cancer therapy using dCas9</p></li>
 
             <li><p>Cell-cell communication through exosomes</p></li>
 
             <li><p>Cell-cell communication through exosomes</p></li>
 
           </ul>
 
           </ul>
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       <div class="timeline-heading">
 
       <div class="timeline-heading">
 
         <h4 class="timeline-title">Brainstorming</h4>
 
         <h4 class="timeline-title">Brainstorming</h4>
−
         <h4 class="timeline-title">26.02.2015 - 12.03.2015</h4>
+
         <h4 class="timeline-title">February 26th - March 12th 2015</h4>
 
       </div>
 
       </div>
 
       <div class="timeline-body">
 
       <div class="timeline-body">
−
         <p>The team is split into small groups, brainstorming for an interesting project that will occupy us during the summer.</p>
+
         <p>The team is split into small groups to brainstorm for an interesting project.</p>
 
       </div>
 
       </div>
 
     </div>
 
     </div>
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       <div class="timeline-heading">
 
       <div class="timeline-heading">
 
         <h4 class="timeline-title">First meeting: iGEM officially starts!</h4>
 
         <h4 class="timeline-title">First meeting: iGEM officially starts!</h4>
−
         <h4 class="timeline-title">19.02.2015</h4>
+
         <h4 class="timeline-title">February 19th 2015</h4>
 
       </div>
 
       </div>
 
       <div class="timeline-body">
 
       <div class="timeline-body">
−
         <p>First meeting with everyone: 12 teammates, 7 TAs and 3 advisors.</p>
+
         <p>First meeting with everyone: 12 teammates, 7 advisors and 3 instructors.</p>
 
         <img src="https://static.igem.org/mediawiki/2015/2/2e/Team_All.jpg" width="100%">
 
         <img src="https://static.igem.org/mediawiki/2015/2/2e/Team_All.jpg" width="100%">
 
       </div>
 
       </div>

Latest revision as of 10:10, 31 August 2015

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

Timeline

  • Our project has a name!

    July 8th 2015

    After a few looongs discussions and many ideas we choose our name:
    Bio LOGIC - Logic Orthogonal gRNA-Implemented Circuits

  • Get back to work!

    July 1st 2015

    First of many meetings this summer! The day after our exams finished, we all got together to set some goals for the next few weeks. Let the fun begin!

  • Break time

    June 1st - July 1st 2015

    Our project was put on hold during our exams. :(

  • Working in the lab

    April 21st - May 31st 2015

    Lab work is slow because we have to follow all our courses at the same time. We are able to make our first construct pdCas9-w after mastering the art of PCR and Gibson assembly!

  • Presents!

    April 14th 2015

    The E. Coli part of our project is strongly based on the paper of Bikard et al. "Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system". Bikard sent us his strains JEN202, JEN202+PWJ89 and DH5A+PWJ66. What a generous guy!

  • Blast

    April 2nd 2015

    In our project dCas9-w targets DNA sequences using gRNAs and acts as an activator or an inhibitor depending on its position. Axel and Cyril created a C++ program to blast random generated gRNA sequences on the E. Coli or Yeast genome.

  • And our project is...

    March 26th 2015

    ...the new framework for biologic circuits using dCas9-w as a synthetic transcription factor. Go to our project descriptionpage to find out more!

  • The lab starts!

    March 24th -31st 2015

    Advisors start to train us in the lab: LB medium and LB agar plates preparation, bacterial cultures, minipreps, PCR, etc.

  • Brainstorming finalists

    March 19th 2015

    Three cool ideas came out of the brainstorming:

    • A new framework for biologic circuits

    • Lung cancer therapy using dCas9

    • Cell-cell communication through exosomes

  • Brainstorming

    February 26th - March 12th 2015

    The team is split into small groups to brainstorm for an interesting project.

  • First meeting: iGEM officially starts!

    February 19th 2015

    First meeting with everyone: 12 teammates, 7 advisors and 3 instructors.

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

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