Difference between revisions of "Team:EPF Lausanne/Timeline"

 
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        <h2>Timeline</h2>
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    <p class="chromeframe">You are using an <strong>outdated</strong> browser. Please <a href="http://browsehappy.com/">upgrade your browser</a> or <a href="http://www.google.com/chromeframe/?redirect=true">activate Google Chrome Frame</a> to improve your experience.</p>
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                        </button>
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                        <a class="navbar-brand" href="https://2015.igem.org/Team:EPF_Lausanne">Bio Logic</a>
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                            <li><a href="https://2015.igem.org/Team:EPF_Lausanne">Home</a></li>
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                            <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Project <span class="caret"></span></a>
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                                <li><a href="https://2015.igem.org/Team:EPF_Lausanne/Project/Overview">Overview</a></li>
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                                <li><a href="#">Organisms</a></li>
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                                <li><a href="#">Applications</a></li>
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                                <li><a href="#">Modelling</a></li>
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                            <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Achievements <span class="caret"></span></a>
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                                <li><a href="#">Results</a></li>
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                                <li><a href="#">Data</a></li>
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                                <li><a href="#">Judging</a></li>
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                            <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Policy and Practices <span class="caret"></span></a>
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                                <li><a href="#">Human Practices</a></li>
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                                <li><a href="#">Safety</a></li>
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                            <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Notebook <span class="caret"></span></a>
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                                <li><a href="#">Yeast</a></li>
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                                <li><a href="#">E.coli</a></li>
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                                <li><a href="#">Protocols</a></li>
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                            <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Team <span class="caret"></span></a>
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                                <li><a href="#">Meet us</a></li>
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                                <li><a href="#">Attributions</a></li>
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                                <li><a href="#">Aknowledgements</a></li>
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                                <li><a href="https://2015.igem.org/Team:EPF_Lausanne/Timeline">Timeline</a></li>
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                            </ul>
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                    <h3>Overview</h3>
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        <ul class="timeline">
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              <h4 class="timeline-title">Mussum ipsum cacilds</h4>
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        <h4 class="timeline-title">Our project has a name!</h4>
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              <p><small class="text-muted"><i class="glyphicon glyphicon-time"></i> 11 hours ago via Twitter</small></p>
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         <h4 class="timeline-title">July 8th 2015</h4>
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              <p>Mussum ipsum cacilds, vidis litro abertis. Consetis adipiscings elitis. Pra lá , depois divoltis porris, paradis. Paisis, filhis, espiritis santis. Mé faiz elementum girarzis, nisi eros vermeio, in elementis mé pra quem é amistosis quis leo. Manduma pindureta quium dia nois paga. Sapien in monti palavris qui num significa nadis i pareci latim. Interessantiss quisso pudia ce receita de bolis, mais bolis eu num gostis.</p>
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         <p>After a few looongs discussions and many ideas we choose our name:</br><b>Bio LOGIC - Logic Orthogonal gRNA-Implemented Circuits</b></p>
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              <h4 class="timeline-title">Mussum ipsum cacilds</h4>
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            </div>
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            <div class="timeline-body">
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              <p>Mussum ipsum cacilds, vidis litro abertis. Consetis adipiscings elitis. Pra lá , depois divoltis porris, paradis. Paisis, filhis, espiritis santis. Mé faiz elementum girarzis, nisi eros vermeio, in elementis mé pra quem é amistosis quis leo. Manduma pindureta quium dia nois paga. Sapien in monti palavris qui num significa nadis i pareci latim. Interessantiss quisso pudia ce receita de bolis, mais bolis eu num gostis.</p>
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              <p>Suco de cevadiss, é um leite divinis, qui tem lupuliz, matis, aguis e fermentis. Interagi no mé, cursus quis, vehicula ac nisi. Aenean vel dui dui. Nullam leo erat, aliquet quis tempus a, posuere ut mi. Ut scelerisque neque et turpis posuere pulvinar pellentesque nibh ullamcorper. Pharetra in mattis molestie, volutpat elementum justo. Aenean ut ante turpis. Pellentesque laoreet mé vel lectus scelerisque interdum cursus velit auctor. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Etiam ac mauris lectus, non scelerisque augue. Aenean justo massa.</p>
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              <h4 class="timeline-title">Mussum ipsum cacilds</h4>
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              <p>Mussum ipsum cacilds, vidis litro abertis. Consetis adipiscings elitis. Pra lá , depois divoltis porris, paradis. Paisis, filhis, espiritis santis. Mé faiz elementum girarzis, nisi eros vermeio, in elementis mé pra quem é amistosis quis leo. Manduma pindureta quium dia nois paga. Sapien in monti palavris qui num significa nadis i pareci latim. Interessantiss quisso pudia ce receita de bolis, mais bolis eu num gostis.</p>
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              <h4 class="timeline-title">Mussum ipsum cacilds</h4>
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              <p>Mussum ipsum cacilds, vidis litro abertis. Consetis adipiscings elitis. Pra lá , depois divoltis porris, paradis. Paisis, filhis, espiritis santis. Mé faiz elementum girarzis, nisi eros vermeio, in elementis mé pra quem é amistosis quis leo. Manduma pindureta quium dia nois paga. Sapien in monti palavris qui num significa nadis i pareci latim. Interessantiss quisso pudia ce receita de bolis, mais bolis eu num gostis.</p>
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              <h4 class="timeline-title">Mussum ipsum cacilds</h4>
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              <p>Mussum ipsum cacilds, vidis litro abertis. Consetis adipiscings elitis. Pra lá , depois divoltis porris, paradis. Paisis, filhis, espiritis santis. Mé faiz elementum girarzis, nisi eros vermeio, in elementis mé pra quem é amistosis quis leo. Manduma pindureta quium dia nois paga. Sapien in monti palavris qui num significa nadis i pareci latim. Interessantiss quisso pudia ce receita de bolis, mais bolis eu num gostis.</p>
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                  <i class="glyphicon glyphicon-cog"></i> <span class="caret"></span>
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                  <li><a href="#">Action</a></li>
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                  <li><a href="#">Another action</a></li>
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                  <li><a href="#">Something else here</a></li>
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                  <li><a href="#">Separated link</a></li>
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              <p>Mussum ipsum cacilds, vidis litro abertis. Consetis adipiscings elitis. Pra lá , depois divoltis porris, paradis. Paisis, filhis, espiritis santis. Mé faiz elementum girarzis, nisi eros vermeio, in elementis mé pra quem é amistosis quis leo. Manduma pindureta quium dia nois paga. Sapien in monti palavris qui num significa nadis i pareci latim. Interessantiss quisso pudia ce receita de bolis, mais bolis eu num gostis.</p>
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              <h4 class="timeline-title">Mussum ipsum cacilds</h4>
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            <div class="timeline-body">
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              <p>Mussum ipsum cacilds, vidis litro abertis. Consetis adipiscings elitis. Pra lá , depois divoltis porris, paradis. Paisis, filhis, espiritis santis. Mé faiz elementum girarzis, nisi eros vermeio, in elementis mé pra quem é amistosis quis leo. Manduma pindureta quium dia nois paga. Sapien in monti palavris qui num significa nadis i pareci latim. Interessantiss quisso pudia ce receita de bolis, mais bolis eu num gostis.</p>
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                    <h2>Still under construction</h2>
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        <h4 class="timeline-title">Get back to work!</h4>
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        <h4 class="timeline-title">July 1st 2015</h4>
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      <div class="timeline-body">
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         <p>First of many meetings this summer! The day after our exams finished, we all got together to set some goals for the next few weeks. Let the fun begin!</p>
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        <h4 class="timeline-title">Break time</h4>
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        <h4 class="timeline-title">June 1st - July 1st 2015</h4>
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      <div class="timeline-body">
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        <p>Our project was put on hold during our exams. :(</p>
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    <div class="timeline-badge warning"></div>
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        <h4 class="timeline-title">Working in the lab</h4>
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        <h4 class="timeline-title">April 21st - May 31st 2015</h4>
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      <div class="timeline-body">
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        <p>Lab work is slow because we have to follow all our courses at the same time. We are able to make our first construct pdCas9-w after mastering the art of PCR and Gibson assembly!</p>
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        <h4 class="timeline-title">Presents!</h4>
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        <h4 class="timeline-title">April 14th 2015</h4>
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      </div>
 +
      <div class="timeline-body">
 +
        <p>The E. Coli part of our project is strongly based on the paper of Bikard et al. "Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system". Bikard sent us his strains JEN202, JEN202+PWJ89 and DH5A+PWJ66. What a generous guy!</p>
 +
      </div>
 +
    </div>
 +
    </li>
 +
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 +
    <div class="timeline-badge danger"></div>
 +
    <div class="timeline-panel">
 +
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 +
        <h4 class="timeline-title">Blast</h4>
 +
        <h4 class="timeline-title">April 2nd 2015</h4>
 +
      </div>
 +
      <div class="timeline-body">
 +
        <p>In our project dCas9-w targets DNA sequences using gRNAs and acts as an activator or an inhibitor depending on its position. Axel and Cyril created a C++ program to blast random generated  gRNA sequences on the E. Coli or Yeast genome.</p>
 +
      </div>
 +
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 +
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 +
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        <h4 class="timeline-title">And our project is...</h4>
 +
        <h4 class="timeline-title">March 26th 2015</h4>
 +
      </div>
 +
      <div class="timeline-body">
 +
        <p>...the new framework for biologic circuits using dCas9-w as a synthetic transcription factor. Go to our <a href="https://2015.igem.org/Team:EPF_Lausanne/Project/Description">project description</a>page to find out more!</p>
 +
        <img src="https://static.igem.org/mediawiki/2015/9/96/Ari_dCas9.jpg" width="100%">
 +
      </div>
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        <h4 class="timeline-title">The lab starts!</h4>
 +
        <h4 class="timeline-title">March 24th -31st 2015</h4>
 +
      </div>
 +
      <div class="timeline-body">
 +
        <p>Advisors start to train us in the lab: LB medium and LB agar plates preparation, bacterial cultures, minipreps, PCR, etc.</p>
 +
      </div>
 +
    </div>
 +
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        <h4 class="timeline-title">Brainstorming finalists</h4>
 +
        <h4 class="timeline-title">March 19th 2015</h4>
 +
      </div>
 +
      <div class="timeline-body">
 +
        <p>Three cool ideas came out of the brainstorming:
 +
          <ul>
 +
            <li><p>A new framework for biologic circuits</p></li>
 +
            <li><p>Lung cancer therapy using dCas9</p></li>
 +
            <li><p>Cell-cell communication through exosomes</p></li>
 +
          </ul>
 +
        </p>
 +
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 +
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    <div class="timeline-panel">
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        <h4 class="timeline-title">Brainstorming</h4>
 +
        <h4 class="timeline-title">February 26th - March 12th 2015</h4>
 +
      </div>
 +
      <div class="timeline-body">
 +
        <p>The team is split into small groups to brainstorm for an interesting project.</p>
 +
      </div>
 +
    </div>
 +
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 +
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        <h4 class="timeline-title">First meeting: iGEM officially starts!</h4>
 +
        <h4 class="timeline-title">February 19th 2015</h4>
 +
      </div>
 +
      <div class="timeline-body">
 +
        <p>First meeting with everyone: 12 teammates, 7 advisors and 3 instructors.</p>
 +
        <img src="https://static.igem.org/mediawiki/2015/2/2e/Team_All.jpg" width="100%">
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Latest revision as of 10:10, 31 August 2015

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

Timeline

  • Our project has a name!

    July 8th 2015

    After a few looongs discussions and many ideas we choose our name:
    Bio LOGIC - Logic Orthogonal gRNA-Implemented Circuits

  • Get back to work!

    July 1st 2015

    First of many meetings this summer! The day after our exams finished, we all got together to set some goals for the next few weeks. Let the fun begin!

  • Break time

    June 1st - July 1st 2015

    Our project was put on hold during our exams. :(

  • Working in the lab

    April 21st - May 31st 2015

    Lab work is slow because we have to follow all our courses at the same time. We are able to make our first construct pdCas9-w after mastering the art of PCR and Gibson assembly!

  • Presents!

    April 14th 2015

    The E. Coli part of our project is strongly based on the paper of Bikard et al. "Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system". Bikard sent us his strains JEN202, JEN202+PWJ89 and DH5A+PWJ66. What a generous guy!

  • Blast

    April 2nd 2015

    In our project dCas9-w targets DNA sequences using gRNAs and acts as an activator or an inhibitor depending on its position. Axel and Cyril created a C++ program to blast random generated gRNA sequences on the E. Coli or Yeast genome.

  • And our project is...

    March 26th 2015

    ...the new framework for biologic circuits using dCas9-w as a synthetic transcription factor. Go to our project descriptionpage to find out more!

  • The lab starts!

    March 24th -31st 2015

    Advisors start to train us in the lab: LB medium and LB agar plates preparation, bacterial cultures, minipreps, PCR, etc.

  • Brainstorming finalists

    March 19th 2015

    Three cool ideas came out of the brainstorming:

    • A new framework for biologic circuits

    • Lung cancer therapy using dCas9

    • Cell-cell communication through exosomes

  • Brainstorming

    February 26th - March 12th 2015

    The team is split into small groups to brainstorm for an interesting project.

  • First meeting: iGEM officially starts!

    February 19th 2015

    First meeting with everyone: 12 teammates, 7 advisors and 3 instructors.

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

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