Difference between revisions of "Team:EPF Lausanne/Ari/enjoy"

 
(256 intermediate revisions by the same user not shown)
Line 1: Line 1:
<!--DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">-->
+
{{:Team:EPF_Lausanne/Ari/Top_Nav}}
 
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
 
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
  <head>
 
<style type="text/css">
 
  
  #contentSub, #footer-box, #catlinks, #search-controls,
+
<body data-spy="scroll" data-target="#myScrollspy" data-offset="20">
  #p-logo, .printfooter, .firstHeading, .visualClear {
+
      display: none;
+
  }
+
  #globalWrapper, #content {   
+
    width: 100%;
+
    height: 100%;
+
    border: 0px;
+
    background-color: #669999;
+
    margin: -7px;
+
    padding: 0px;
+
    font-size: 100%;
+
  }
+
img.resize50{
+
  max-width:50%;
+
  max-height:50%;
+
}
+
  
img.resize30{
+
<div id="full-size">
    max-width:30%;
+
    <div id="cont">  
    max-height:30%;
+
}
+
</style>
+
    <!--
+
    Stone Template
+
    http://www.templatemo.com/preview/templatemo_452_stone
+
    -->
+
 
+
    <!-- Latest compiled and minified CSS -->
+
    <link rel="stylesheet" href="http://maxcdn.bootstrapcdn.com/bootstrap/3.3.5/css/bootstrap.min.css">
+
   
+
    <!-- Bootstrap Stylesheet -->
+
    <link rel="stylesheet" href="https://2015.igem.org/Team:EPF_Lausanne/Test/css/bootstrap?action=raw&amp;ctype=text/css" type="text/css">
+
   
+
    <!-- FontAwesome Icons -->
+
    <link rel="stylesheet" href="http://maxcdn.bootstrapcdn.com/font-awesome/4.3.0/css/font-awesome.min.css">
+
   
+
    <!-- Normailize Stylesheet -->
+
    <link rel="stylesheet" href="https://2015.igem.org/Team:EPF_Lausanne/Test/css/normalize?action=raw&amp;ctype=text/css" type="text/css">
+
 
+
    <!-- Main Styles -->
+
    <link rel="stylesheet" href="https://2015.igem.org/Team:EPF_Lausanne/Ari/css/main?action=raw&amp;ctype=text/css" type="text/css">
+
 
+
    <!-- Footer -->
+
    <link rel="stylesheet" href="https://2015.igem.org/Team:EPF_Lausanne/css/footer?action=raw&amp;ctype=text/css" type="text/css">
+
 
+
    <script src="https://2015.igem.org/Team:EPF_Lausanne/Test/js/mordernizr-2.6.2?action=raw&amp;ctype=text/js"></script>
+
 
+
</head>
+
<body  class="loading">
+
    <!--[if lt IE 7]>
+
    <p class="chromeframe">You are using an <strong>outdated</strong> browser. Please <a href="http://browsehappy.com/">upgrade your browser</a> or <a href="http://www.google.com/chromeframe/?redirect=true">activate Google Chrome Frame</a> to improve your experience.</p>
+
    <![endif]-->
+
 
+
    <!-- HEADER -->
+
    <div class="site-header">
+
        <div class="container">
+
 
             <div class="row">
 
             <div class="row">
                 <nav class="navbar navbar-default navbar-static-top">
+
                 <div class="col-md-6">
                     <div class="navbar-header">
+
                     <img class="img-responsive" src="https://static.igem.org/mediawiki/2015/5/5c/EPF_Lausanne_Arbre.png">
                        <button class="navbar-toggle" type="button" data-toggle="collapse" data-target="#main-menu">
+
                </div>
                            <span class="sr-only">Toggle navigation</span>
+
                <div class="col-md-6" id="Welcome-section">
                            <i class="fa fa-bars"></i>
+
                    <h1>Bio LOGIC</h1>
                        </button>
+
                    <h2>Logic Orthogonal gRNA Implemented Circuits</h2>
                        <!--<a class="navbar-brand" href="https://2015.igem.org/Team:EPF_Lausanne">Bio Logic</a>-->
+
                    <p>Engineering transcriptional logic gates to program cellular behavior remains an important challenge for synthetic biology. Currently, genetic circuits reproducing digital logic are limited in scalability and robustness by output discrepancies and crosstalk between transcriptional pathways. We propose to address these issues using RNA-guided dCas9 fused to a transcription activation domain as a programmable transcription factor.</p>
                        <a height="15" href="https://2015.igem.org/Team:EPF_Lausanne"><img src="https://static.igem.org/mediawiki/2015/9/95/EPF_Lausanne_BIOLOGIC_BLANC.png"></a>
+
                    <h3>Read More</h3>
 +
                    <a href="#Start"><i class="fa fa-arrow-circle-down fa-3x"></i></a>
 +
                </div>
 +
            </div>
 +
      </div>
 +
</div> <!--/Welcome-section-->
 +
<div id="Start" class="home-first-section">
 +
    <div class="container">
 +
        <div class="row">
 +
            <div class="col-md-12 col-centered">
 +
            <div class="col-md-12">
 +
                    <h1>Project</h1>
 +
                    <div class="col-md-3">
 +
                      <a href="https://2015.igem.org/Team:EPF_Lausanne/Project/Background">
 +
                        <div class="panel">
 +
                        <i class="center fa fa-history fa-2x"></i>
 +
                        <h2>Background</h2>
 +
                          <p>Discover the ideas behind our project, from boolean logic to the the complete explanation of a biologic logic gate.</p>
 +
                        </div>
 +
                      </a>
 
                     </div>
 
                     </div>
                     <div class="collapse navbar-collapse" id="main-menu">
+
                     <div class="col-md-3">
                        <ul class="nav navbar-nav navbar-right"> <li><a href="https://2015.igem.org/Team:EPF_Lausanne">Home</a></li>
+
                      <a href="https://2015.igem.org/Team:EPF_Lausanne/Description">
 
+
                        <div class="panel">
                            <!--<li><a href="https://2015.igem.org/Team:EPF_Lausanne">Home</a></li>-->
+
                        <i class="center fa fa-search fa-2x"></i>
                            <li class="dropdown">
+
                        <h2>Description</h2>
                            <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Project <span class="caret"></span></a>
+
                          <p>Understand our project in the smallest details and explore the world of CRISPR-dCas9 with us.</p>
                            <ul class="dropdown-menu">
+
                        </div>
<li><a href="https://2015.igem.org/Team_EPF_Lausanne/Project/Background">Background</a></li>
+
                      </a>
                                <li><a href="https://2015.igem.org/Team:EPF_Lausanne/Project/Description">Description</a></li>
+
                                <li><a href="https://2015.igem.org/Team:EPF_Lausanne/Project/Applications">Applications</a></li>
+
                                <li><a href="https://2015.igem.org/Team:EPF_Lausanne/Project/Modelling">Modelling</a></li>
+
                            </ul>
+
                            </li>
+
                            <li class="dropdown">
+
                            <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Achievements <span class="caret"></span></a>
+
                            <ul class="dropdown-menu">
+
                                <li><a href="https://2015.igem.org/Team:EPF_Lausanne/Achievements/Vivo">In vivo</a></li>
+
                                <li><a href="https://2015.igem.org/Team:EPF_Lausanne/Achievements/Silico">In silico</a></li>
+
                                <li><a href="https://2015.igem.org/Team:EPF_Lausanne/Achievements/Judging">Judging</a></li>
+
                                <li><a href="https://2015.igem.org/Team:EPF_Lausanne/Achievements/Parts">Parts</a></li>
+
                            </ul>
+
                            </li>
+
                            <li class="dropdown">
+
                            <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Policy and Practices <span class="caret"></span></a>
+
                            <ul class="dropdown-menu">
+
                                <li><a href="https://2015.igem.org/Team:EPF_Lausanne/Policy/Ethic">Ethics</a></li>
+
                                <li><a href="https://2015.igem.org/Team:EPF_Lausanne/Policy/Safety">Safety</a></li>
+
                                <li><a href="https://2015.igem.org/Team:EPF_Lausanne/Policy/Human">Human Practices</a></li>
+
                            </ul>
+
                            </li>
+
                            <li class="dropdown">
+
                            <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Notebook <span class="caret"></span></a>
+
                            <ul class="dropdown-menu">
+
                                <li><a href="https://2015.igem.org/Team:EPF_Lausanne/Notebook/Ecoli">E. Coli</a></li>
+
                                <li><a href="https://2015.igem.org/Team:EPF_Lausanne/Notebook/Yeast">Yeast</a></li>
+
                                <li><a href="https://2015.igem.org/Team:EPF_Lausanne/Notebook/Protocols">Protocols</a></li>
+
                            </ul>
+
                            </li>
+
                            <li class="dropdown">
+
                            <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Team <span class="caret"></span></a>
+
                            <ul class="dropdown-menu">
+
                                <li><a href="https://2015.igem.org/Team:EPF_Lausanne/Team/Meet">Meet us</a></li>
+
                                <li><a href="https://2015.igem.org/Team:EPF_Lausanne/Timeline">Timeline</a></li>
+
                                <li><a href="https://2015.igem.org/Team:EPF_Lausanne/Team/Aknowledgements">Aknowledgements</a></li>
+
                            </ul>
+
                            </li>
+
                        </ul>
+
 
                     </div>
 
                     </div>
                </nav>        
+
                    <div class="col-md-3">
            </div>
+
                      <a href="https://2015.igem.org/Team:EPF_Lausanne/Project/Applications">
        </div>
+
                        <div class="panel">
    </div> <!-- .site-header -->
+
                        <i class="center fa fa-rocket fa-2x"></i>
 
+
                        <h2>Applications</h2>
    <!-- SLIDER -->
+
                          <p>Need a biosensor? Want a bacteria that can depollute a lake? Here are a few examples of how you could apply our technology for your own designs.</p>
    <div class="site-slider">
+
                        </div>
        <div class="flexslider">
+
                      </a>
            <ul class="slides">
+
                    </div>
                <li class="slide">
+
                     <div class="col-md-3">
                     <div class="overlay"></div>
+
                      <a href="https://2015.igem.org/Team:EPF_Lausanne/Modeling">
                    <img src="https://static.igem.org/mediawiki/2015/f/ff/EPF_Lausanne_RLC_BW.jpg" alt="">
+
                        <div class="panel">
                    <div class="slider-caption">
+
                        <i class="center fa fa-code fa-2x"></i>
                        <!--<div class="title">
+
                        <h2>Modeling</h2>
                            <h2>Find out about our project</h2>
+
                          <p>Having a robust system is the key when devising new concepts. It enables testing in silico before ordering the expensive parts...</p>
                        </div>-->
+
                         </div>
                         <a href="https://2015.igem.org/Team:EPF_Lausanne/Project/Description" class="slider-button">Find out about our project</a>
+
                      </a>
 
                     </div>
 
                     </div>
                </li>
+
            </div>
            </ul>
+
              <div class="col-md-12">
        </div>  
+
                <h1>Achievements</h1>
    </div> <!-- .site-slider -->
+
                 <div class="col-md-3">
 
+
                  <a href="https://2015.igem.org/Team:EPF_Lausanne/Achivements/Vivo">
    <div id="Start" class="second-section">
+
                     <div class="panel">
        <div class="container">
+
                    <i class="center fa fa-flask fa-2x"></i>
            <div class="row">
+
                    <h2>In Vivo</h2>
                 <div class="col-md-8 col-centered">
+
                      <p>Our achievements in the wet-lab, the result of a summer in the cold labrooms.</p>
                    <h3>Biologic Orthogonal GRNA-Implemented Circuit</h3>
+
                     </div>
                    <p>This summer, the EPFL iGEM team strives to pave the way for simpler implementation of digital circuits in vivo. Using the newly discovered dCas9 as a synthetic transcription factor, we aim to design biocompatible transistor-like elements. Our ultimate goal is to make cells smarters by assembling these transistors into logic gates that are both chainable and parallelizable in a homogenous logic framework.</p>
+
                  </a>
                     <h2>Thinking Binary</h2>
+
                    <p>Boolean Logic is the bedrock of the digital revolution. Developed by George Boole in the mid-19th century, it is based on a simple set of values: 0 (“FALSE”) or 1 (“TRUE”). Modern computers represent all forms of information using strings of the same 0s and 1s (also named “Bits”). The processing of bits is handled by logical transistors - which can be seen as electronically controllable switches. Elementary logic operation are performed using cleverly assembled transistors. Such assemblies are named “logic gates”. Gates are the bricks with which complex behaviour is produced.</p>
+
                    <h2>Biological computers</h2>
+
                    <p>Since the early 2000’s, multiple synthetic biological gates have been built, revolutionizing our ability to dictate the way organisms react to stimuli. Their applications range from intelligent biosensors to cellular therapeutics with improved in vivo targeting and curing.<br>
+
                    Unfortunately, the development of programmable cells has been hampered by difficulties in the multiplication and chaining of logic elements. This has hindered the complexification of bio-circuits as well as the automation and flexibility of their design.<br>
+
                    To overcome these limitations, an ideal in vivo logic element should be modular, reusable, and orthogonal - i.e avoiding unwanted cross-talk with its host organism as well as other elements of the engineered circuit.</p>
+
                     <h2>Cas9 Logic Gates</h2>
+
                    <p>Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease that targets and cleaves any DNA sequence complementary to its guide RNA (gRNA). Our project will be based upon a derivative of this technology : catalytically “dead” Cas9 (dCas9) that lack the ability to cleave DNA. When fused to a RNA polymerase (RNAP) recruiting element (e.g. the omega subunit of RNAP in E. Coli or VP64 in eukaryotes), chimeric dCas9 can act as a  programmable transcription activator. In addition, activating dCas9 may also act as a DNA transcription inhibitor: depending on its gRNA-determined binding site, it has been shown in yeasts to sterically hinder RNAP recruitment to promoter sequences.<br>
+
                    Exploiting dCas9-omega/VP64’s ambivalence, we propose the creation of gRNA-controlled switch-like elements analogous to transistors. The state of the switch would be solely dependent on the position of dCas9 relative to the promoter. The content of the gRNA-targeted sequences might therefore be designed such that each transistor is orthogonal to other logic elements. Using gRNA to make what could be seen as “biological wires”,  we also hope to achieve chainability of the transistors and thus complexification of bio-circuits.</p>
+
 
                 </div>
 
                 </div>
            </div>
+
                <div class="col-md-3">
        </div>
+
                  <a href="https://2015.igem.org/Team:EPF_Lausanne/Achievements/Silico">
    </div>
+
                    <div class="panel">
    <div class="fourth-section">
+
                    <i class="center fa fa-laptop fa-2x"></i>
        <div class="container">
+
                    <h2>In silico</h2>
            <div class="row">
+
                      <p>What we were able to do using the computer.</p>
                <div class="col-md-12 text-center">
+
                    </div>
                    <h2>Still under construction</h2>
+
                  </a>
 
                 </div>
 
                 </div>
            </div>
+
                <div class="col-md-3">
        </div>
+
                    <a href="https://2015.igem.org/Team:EPF_Lausanne/Achievements/Judging">
    </div>
+
                      <div class="panel">
 
+
                      <i class="center fa fa-legal fa-2x"></i>
    <!-- SPONSORS -->
+
                      <h2>Judging</h2></a>
<div class="sponsors">
+
                        <p>The criterias that we fulfilled for the different medals.</p>
<div><h1 class="cntr">Sponsors</h1></div>
+
                      </div>
  <div class="container">
+
                     </a>
    <div class="row">
+
<div class="col-lg-12">
+
<ul class="list-inline">
+
<li><a href="http://www.epfl.ch/" target="_blank"><img src="https://static.igem.org/mediawiki/2014/thumb/0/02/EPFL_logo.png/800px-EPFL_logo.png" height="100"></a></li>
+
<li><a href="http://www.novartis.ch/" target="_blank"><img src="https://static.igem.org/mediawiki/2014/7/7f/Novartis.png" height="70"></a></li>
+
<li><a href="http://www.roche.com/index.htm" target="_blank"><img src="https://static.igem.org/mediawiki/2014/1/13/Roche.png" height="100"></a></li>
+
<li><a href="http://www.nikon.ch/" target="_blank"><img src="https://static.igem.org/mediawiki/2014/f/f7/Nikon.png" height="100"></a></li>
+
<!--<li><a href="http://www.sbg-marcom.ch/" target="_blank"><img src="https://static.igem.org/mediawiki/2014/d/d0/SBG.gif" height="100"></a></li>-->
+
<li><a href="http://www.microsynth.ch/" target="_blank"><img src="https://static.igem.org/mediawiki/2014/6/68/Microsynth_logo_20kpix.png" height="50"></a></li>
+
<li><a href="http://www.snapgene.com/" target="_blank"><img src="https://static.igem.org/mediawiki/2014/3/35/SnapGene_Logo_20kpix.png" height="50"></a></li>
+
</ul>
+
</div>
+
</div>
+
    </div>
+
    <footer class="footer-distributed">
+
 
+
            <div class="footer-left">
+
 
+
                <img class="resize50" src="https://static.igem.org/mediawiki/2015/4/4e/EPF_Lausanne_iGem_Logo_Blue.png" alt="">
+
 
+
            </div>
+
 
+
            <div class="footer-center">
+
 
+
                <div>
+
                     <i class="fa fa-map-marker"></i>
+
                    <p><span>Route Cantonale</span> Lausanne, Suisse</p>
+
 
                 </div>
 
                 </div>
 
+
                 <div class="col-md-3">
                 <div>
+
                     <a href="https://2015.igem.org/Team:EPF_Lausanne/Achievements/Parts">
                     <i class="fa fa-envelope"></i>
+
                      <div class="panel">
                    <p><a href="mailto:iGEM2015dreamteam@groupes.epfl.ch">Contact us</a></p>
+
                      <i class="center fa fa-puzzle-piece fa-2x"></i>
 +
                        <h2>Parts</h2>
 +
                        <p>iGEM is all about Biobricks. Here is our contribution to this expanding library.</p>
 +
                      </div>
 +
                    </a>
 +
                </div>
 +
            </div>
 +
              <div class="col-md-12">
 +
                <h1>Policy and Practices</h1>
 +
                <div class="col-md-4">
 +
                <a href="https://2015.igem.org/Team:EPF_Lausanne/Practices">
 +
                    <div class="panel">
 +
                  <i class="center fa fa-eye fa-2x"></i>
 +
                  <h2>Ethics</h2>
 +
                    <p>Is working on the genome ethically correct? We tried to answer to this question.</p>
 +
                    </div>
 +
                  </a>
 
                 </div>
 
                 </div>
 +
                <div class="col-md-4">
 +
                <a href="https://2015.igem.org/Team:EPF_Lausanne/Practices">
 +
                    <div class="panel">
 +
                  <i class="center fa fa-bomb fa-2x"></i>
 +
                  <h2>Safety</h2>
 +
                    <p>Doing safe work with small organisms can be dangerous. Here is what we did to protect ourselves and the environment.</p>
 +
                    </div>
 +
                  </a>
 +
                </div>
 +
                <div class="col-md-4">
 +
                <a href="https://2015.igem.org/Team:EPF_Lausanne/Practices">
 +
                    <div class="panel">
 +
                  <i class="center fa fa-comments fa-2x"></i>
 +
                  <h2>Human Practices</h2>
 +
                    <p>We do good ! Don't listen to the mass media.</p>
 +
                    </div>
 +
                  </a>
 +
                </div>
 +
            </div>
  
            </div>
+
              <div class="col-md-12">
 +
              <h1>Notebook</h1>
 +
                  <div class="col-md-4">
 +
                  <a href="https://2015.igem.org/Team:EPF_Lausanne/Notebook/Ecoli">
 +
                      <div class="panel">
 +
                    <i class="center fa fa-bug fa-2x"></i>
 +
                    <h2>E. coli</h2>
 +
                      <p>See how we planned our summer and how it went for the E.coli team.</p>
 +
                      </div>
 +
                    </a>
 +
                  </div>
 +
                  <div class="col-md-4">
 +
                  <a href="https://2015.igem.org/Team:EPF_Lausanne/Notebook/Yeast">
 +
                      <div class="panel">
 +
                    <i class="center fa fa-beer fa-2x"></i>
 +
                    <h2>Yeast</h2>
 +
                      <p>Even if we used a beer icon Yeasts are not all about beer, discover how we experimented with this tough organism.</p>
 +
                      </div>
 +
                    </a>
 +
                  </div>
 +
                  <div class="col-md-4">
 +
                  <a href="https://2015.igem.org/Team:EPF_Lausanne/Notebook/Protocols">
 +
                      <div class="panel">
 +
                    <i class="center fa fa-comments fa-2x"></i>
 +
                    <h2>Protocols</h2>
 +
                      <p>These are the protocols that you need if you want to redo our work.</p>
 +
                      </div>
 +
                    </a>
 +
                  </div>
 +
              </div>
  
            <div class="footer-right">
+
                <div class="col-md-12">
 
+
                <h1>Team</h1>
                <p class="footer-company-about">
+
                    <div class="col-md-3">
                    <span>About us</span>
+
                      <a href="https://2015.igem.org/Team:EPF_Lausanne/Team/Meet">
                    Team of a dozen biotechnology students that will attempt to change the world
+
                        <div class="panel">
                </p>
+
                        <i class="center fa fa-users fa-2x"></i>
 
+
                        <h2>Meet us!</h2>
                <div class="footer-icons">
+
                          <p>Brief description of who is who and for what (s)he is renowned.</p>
 
+
                        </div>
                    <a href="https://www.facebook.com/pages/IGEM-EPFL-2015/1598472927106907" target="_blank"><i class="fa fa-facebook"></i></a>
+
                      </a>
                     <a href="https://instagram.com/igem2015epfl/" target="_blank"><i class="fa fa-instagram"></i></a>
+
                    </div>
                     <a href="https://twitter.com/EPFL_iGEM/" target="_blank"><i class="fa fa-twitter"></i></a>
+
                    <div class="col-md-3">
 +
                      <a href="https://2015.igem.org/Team:EPF_Lausanne/Timeline">
 +
                        <div class="panel">
 +
                        <i class="center fa fa-calendar fa-2x"></i>
 +
                        <h2>Timeline</h2>
 +
                          <p>How did our summer go?</p>
 +
                        </div>
 +
                      </a>
 +
                     </div>
 +
                    <div class="col-md-3">
 +
                      <a href="https://2015.igem.org/Team:EPF_Lausanne/Team/Aknowledgement">
 +
                        <div class="panel">
 +
                        <i class="center fa fa-bullhorn fa-2x"></i>
 +
                        <h2>Aknowledgements</h2>
 +
                          <p>We are thankfull to all the people that helped us.</p>
 +
                        </div>
 +
                      </a>
 +
                     </div>
 +
                    <div class="col-md-3">
 +
                      <a href="https://2015.igem.org/Team:EPF_Lausanne/Attributions">
 +
                        <div class="panel">
 +
                        <i class="center fa fa-pie-chart fa-2x"></i>
 +
                        <h2>Attributions</h2>
 +
                          <p>What you do is what you get.</p>
 +
                        </div>
 +
                      </a>
 +
                    </div>
 
                 </div>
 
                 </div>
 
 
             </div>
 
             </div>
 
+
         </div>
         </footer>
+
     </div>
 
+
</div> <!--/home-first-section-->
     <script src="https://2015.igem.org/Team:EPF_Lausanne/Test/js/jquery?action=raw&amp;ctype=text/js"></script>
+
    <script src="https://2015.igem.org/Team:EPF_Lausanne/Test/js/bootstrap?action=raw&amp;ctype=text/js"></script>
+
    <script src="https://2015.igem.org/Team:EPF_Lausanne/Test/js/plugins?action=raw&amp;ctype=text/js"></script>
+
    <script src="https://2015.igem.org/Team:EPF_Lausanne/Test/js/main?action=raw&amp;ctype=text/js"></script>   
+
   
+
 
</body>
 
</body>
 
</html>
 
</html>
 +
{{:Team:EPF_Lausanne/Test/footer}}

Latest revision as of 21:38, 31 August 2015

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

Bio LOGIC

Logic Orthogonal gRNA Implemented Circuits

Engineering transcriptional logic gates to program cellular behavior remains an important challenge for synthetic biology. Currently, genetic circuits reproducing digital logic are limited in scalability and robustness by output discrepancies and crosstalk between transcriptional pathways. We propose to address these issues using RNA-guided dCas9 fused to a transcription activation domain as a programmable transcription factor.

Read More

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

NOT PROOFREAD