Difference between revisions of "Team:EPF Lausanne/Parts"
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− | <a href="https://static.igem.org/mediawiki/2015/2/25/"><img src="https://static.igem.org/mediawiki/2015/2/25/EPF_Lausanne_Parts_dCas9-w.png" style="width: | + | <a href="https://static.igem.org/mediawiki/2015/2/25/"><img src="https://static.igem.org/mediawiki/2015/2/25/EPF_Lausanne_Parts_dCas9-w.png" style="width:15%"></a> |
<h1><b>dCas9-ω</h1> | <h1><b>dCas9-ω</h1> | ||
<p> dCa9s-ω is our main tool to build our project in <i>E. coli</i>. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activator or as an inhibitor when provided the right guide RNA and acting on the promoters we provided. -</p> | <p> dCa9s-ω is our main tool to build our project in <i>E. coli</i>. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activator or as an inhibitor when provided the right guide RNA and acting on the promoters we provided. -</p> |
Revision as of 11:48, 2 September 2015
EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits
dCas9-ω
dCa9s-ω is our main tool to build our project in E. coli. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activator or as an inhibitor when provided the right guide RNA and acting on the promoters we provided. -