Difference between revisions of "Team:Bielefeld-CeBiTec/Primers"

m
m
Line 201: Line 201:
 
<td>GGATAGCGTAACCTTACTTCCGTACTCATGTACGGAGTCAAGCGATATGGAACTCTAGAAGCGGCCGCGAATTC</td>
 
<td>GGATAGCGTAACCTTACTTCCGTACTCATGTACGGAGTCAAGCGATATGGAACTCTAGAAGCGGCCGCGAATTC</td>
 
</tr>
 
</tr>
 +
 +
<tr id="merT_omRFP_fwd">
 +
<td>merT_omRFP__fwd"</td>
 +
<td>CTCCGTACATGAGTACGGAAGTAAGGTTACGCTATCCAATCCTACTAGATTAAAGAGGAGAAATAC</td>
 +
</tr>
 +
 +
<tr id="1C3_pre_merT_rv">
 +
<td>1C3_pre_merT_rv</td>
 +
<td>CTTACTTCCGTACTCATGTACGGAGTCAAGCGATATGGAACTCTAGAAGCGGCCGCGAATTC</td>
 +
</tr>
 +
  
 
<tr id="UTR_merT_fwd">
 
<tr id="UTR_merT_fwd">
Line 206: Line 217:
 
<td>GAGTACGGAAGTAAGGTTACGCTATCCAATCCTACGACAATAATTTTGTTTAACTTTAAAAAAAAAAAAG</td>
 
<td>GAGTACGGAAGTAAGGTTACGCTATCCAATCCTACGACAATAATTTTGTTTAACTTTAAAAAAAAAAAAG</td>
 
</tr>
 
</tr>
 +
 +
<tr id="pSB1C3_kPrm_rev">
 +
<td>pSB1C3_kPrm_rev</td>
 +
<td>CTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA</td>
 +
</tr>
 +
 +
<tr id="pSB1C3-pbrR-fwd">
 +
<td>pSB1C3-pbrR-fwd">
 +
<td>CCACCGCGCACCCGTCCGATTAACTCTAGAAGGGCCGCGAAT</td>
 +
</tr>
 +
 +
<tr id="
  
  
Line 247: Line 270:
 
                 <td>UTR_arsO_rev</td>
 
                 <td>UTR_arsO_rev</td>
 
                 <td>ccttcttttttttttttaaagttaaacaaaattatTAAGTCAAAAACATATATGACTTAACG</td>
 
                 <td>ccttcttttttttttttaaagttaaacaaaattatTAAGTCAAAAACATATATGACTTAACG</td>
−
            </tr>
+
       
−
          <tr id="UTR_noT7_rev">
+
       
−
                <td>UTR_noT7_rev</td>
+
−
                <td>ccttcttttttttttttaaagttaaacaaaattattCTCTAGAAGCGGCCGCGA</td>
+
−
            </tr>
+
 
<tr id="UTR_arsR_fwd">
 
<tr id="UTR_arsR_fwd">
 
<td>UTR_arsR_fwd</td>
 
<td>UTR_arsR_fwd</td>
Line 363: Line 383:
 
                 <td>TTATTATTTATACAGCTCATCCATACC</td>
 
                 <td>TTATTATTTATACAGCTCATCCATACC</td>
 
             </tr>
 
             </tr>
 +
  </tr>
 +
<tr id="UTR_noT7_rev">
 +
<td>UTR_noT7_rev</td>
 +
<td>CCTTCTTTTTTTTTTTTAAAGTTAAAGTTAAACAAAATTATTCTCTAGAAGCGGCCGCGA</td>
 +
</tr>
 +
 
<tr id="UTR_fwd">
 
<tr id="UTR_fwd">
 
                 <td>UTR_fwd</td>
 
                 <td>UTR_fwd</td>
Line 508: Line 534:
 
<td>BlcR-His_rev</td>
 
<td>BlcR-His_rev</td>
 
<td>CCGTGATGGTGATGGTGATGGTAGTACGACATCTAGTATTTCTCCTCTTTCTCTAGTA</td>
 
<td>CCGTGATGGTGATGGTGATGGTAGTACGACATCTAGTATTTCTCCTCTTTCTCTAGTA</td>
 +
</tr>
 +
 +
<tr id="gadR-qRT-fw">
 +
<td>gadR-qRT-fw</td>
 +
<td>TCAATCCCCTGTAACGG</td>
 +
</tr>
 +
 +
<tr id="gadR-qRT-rev">
 +
<td>gadR-qRT-rev</td>
 +
<td>CCAGGCGCACCGAACAAGAC</td>
 +
</tr>
 +
 +
<tr id="gadT-qRT-fw">
 +
<td>gadT-qRT-fw</td>
 +
<td>ATGAGTCAAACAACAGCAAGCATC</td>
 +
</tr>
 +
 +
<tr id="gadT-qRT-rev">
 +
<td>gadT-qRT-rev</td>
 +
<td>CTCAACCACCTTCGGATG</td>
 
</tr>
 
</tr>
  

Revision as of 14:29, 2 September 2015

iGEM Bielefeld 2015


Primers

...

Name Sequence (5´ → 3´)
Standard primers
mRFP1_rev" GGACGACCTTCACCTTCACCTTCACC
SB-prep-2Ea ATGAATTCCAGAAATCATCCTTAGCG
SB-prep-3P-1 GCCGCTGCAGTCCGGCAAAAAA
VF CCACCTGACGTCTAAGAA
VR GTATTACCGCCTTTGAGT
Heavy metals
cueR_rev

cueR_fwd

rcnR_rev

rcnR_fwd

pSB1C3_copAP_fw

pSB1C3_copAP_rev

pSB1C3_cueR_pre

pSB1C3_cueR_suf

pSB1C3_rcnA_fw

pSB1C3_rcnA_rev

pSB1C3_rcnR (rev)

pSB1C3_fwd (rcnR)

RFP_fwd

kPrm_fwd

rcnA_rev

rcnA_UTR_fw" CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTTTTCTCCTCTTTTACTCCCCCC
UTR_mod_fw GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTCTGCGTAAAGGCGAAGAGCTGTTCAC
pbrA_r

pbrR_rev

MerR_fwd

chrB_fwd

Cr_rev

copA_rev

copAP_UTR_rev CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTCTAGTACTTTAAGACAGTTTTG
CrProm_UTR_rev CTTTTTTTTTTTTAAAGTTAAACAAAATTATTGTCGTAGATGTTACTACAATAATGAG
pbrA_UTR_rev CTCCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTCTAGTATTTCTCCTCTTTTTCTCGCGTTGCC
pSB1C3_merT_rev GGATAGCGTAACCTTACTTCCGTACTCATGTACGGAGTCAAGCGATATGGAACTCTAGAAGCGGCCGCGAATTC
merT_omRFP__fwd" CTCCGTACATGAGTACGGAAGTAAGGTTACGCTATCCAATCCTACTAGATTAAAGAGGAGAAATAC
1C3_pre_merT_rv CTTACTTCCGTACTCATGTACGGAGTCAAGCGATATGGAACTCTAGAAGCGGCCGCGAATTC
UTR_merT_fwd GAGTACGGAAGTAAGGTTACGCTATCCAATCCTACGACAATAATTTTGTTTAACTTTAAAAAAAAAAAAG
pSB1C3_kPrm_rev CTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA
pSB1C3-pbrR-fwd"> CCACCGCGCACCCGTCCGATTAACTCTAGAAGGGCCGCGAAT
Arsenic biosensor
Ars_1_rev tatgacttaacgaatgtgtaagtgcagaggCGGATAAAACACATCTGAAAATTC
Ars_2_fwd cctctgcacttacacattcgttaagtcataGGTACTAGTAGCGGCC
Ars_3_fwd cattcgttaagtcatatatgtttttgacttGGTACTAGTAGCGGCC
Ars_3_rev tcaaaaacatatatgacttaacgaatgtgtCGGATAAAACACATCTGAAAATTC
arsRhis_arsR_fwd tatagggaaagaggagaaatactagagATGTCATTTCTGTTACCCATC
arsRhis_arsR2_r ctagtattagtggtgatggtgatgatgACTGCAAATGTTCTTACTG
arsRhis_Trp_fwd CATCATCACCATCACCAC
arsRhis_Trp_rev CTCTAGTATTTCTCCTCTTTCC
UTR_arsO_rev ccttcttttttttttttaaagttaaacaaaattatTAAGTCAAAAACATATATGACTTAACG
UTR_arsR_fwd GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGTCATTTCTGTTACCCATCC
UTR_arsR_rev CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTCCCTATAGTGAGTCGTATTACTC
CFPS
Mut_gap_UTR_fwd AGAATAATCTATGCGTAAAGGCGAAGAG
pBS1C3_RraA_pre ACAAAGCTCGGAAGTATCGTATTTCATCTCTAGAAGCGGCCGCGAATTCC
pBS1C3_RraA_suf TCAGAAGATCCGCTGGATATTGAATGATACTAGTAGCGGCCGCTGCAG
pBS1C3_RraB_pre TTCTTCCAGTTGTTCCGGGTTTGCCATCTCTAGAAGCGGCCGCGAATTCC
pBS1C3_RraB_suf GAAGACGATGACGGAGTTCGCCACTAATACTAGTAGCGGCCGCTGCAG
pZ9_pT7_rne_fwd gtccgcaacctgttgagtaaCTGCAGGAAGGGGATCCG
pZ9_pT7_rne_rev tagtatttctcctctttctctagtatctcCCTATAGTGAGTCGTATTAC
rne_qPCR_fwd1 GTGCTCGGCATAAGCCGAAG
rne_qPCR_fwd2 GCATAAGCCGAAGATATCG
rne_qPCR_fwd3 CCGAAGATATCGGTAGAGTT
rne_qPCR_fwd4 CTGCGCTACGGTAGCGAAAC
rne_qPCR_rev2 TTTGCGCCTCGTTATCATCC
rne_to_pZ9_fwd GATACTAGAGAAAGAGGAGAAATACTAGATGAAAAGAATGTTAATCAACGCAACTC
rne_to_pZ9_rev CCTGCAGTTACTCAACAGGTTGCGGACGCGCAG
Rra_purif_fwd TCAGAAGATCCGCTGGATATTGAATGATACTAGAGCCAGGCATCAAA
RraA_fwd ATGAAATACGATACTTCCGAGCTTTG
RraA_purif_rev ACAAAGCTCGGAAGTATCGTATTTCATGGCGCCCTGAAAATACAG
RraA_rev TCATTCAATATCCAGCGGATCTTC
RraB_fwd ATGGCAAACCCGGAACAACTG
RraB_purif_fwd GAAGACGATGACGGAGTTCGCCACTAATACTAGAGCCAGGCATCAAA
RraB_purif_rev TTCTTCCAGTTGTTCCGGGTTTGCCATGGCGCCCTGAAAATACAG
RraB_rev TTAGTGGCGAACTCCGTCATC
SYFP_only_fwd ATGGTTAGCAAGGGCGAAGA
SYFP_only_rev TTATTATTTATACAGCTCATCCATACC
UTR_noT7_rev CCTTCTTTTTTTTTTTTAAAGTTAAAGTTAAACAAAATTATTCTCTAGAAGCGGCCGCGA
UTR_fwd GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGCGTAAAGGCGAAGAGCTGTTCAC
UTR_rev CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTTCTCCCTATAGTGAGTCGTATTACTCTAGAAG
UTR_RFP_fwd GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTATGGCTTCCTCCGAAGACGTTATC
UTR_SYFP_fwd GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTATGGTTAGCAAGGGCGAAGAAC
Date rape drugs
BlcR_fwd ATGGGCCAAAGGGGCCAAGT
BlcR_rev CTAGTCTTTCTGCGATCGGTACCC
pSB1C3_BlcR_fwd cttgggtaccgatcgcagaaagactagTACTAGTAGCGGCCGCTGCAG
psB_BlcRfwd_new" CTTGGGTACCGATCGCAGAAAGACTAATACTAGTAGCGGCCGCTGCAG
pSB1C3_BlcR_rev ttgacacacttggcccctttggcccatctagtaTTTCTCCTCTTTCTCTAGTAGCTAGCACAATAC
BlcC_rev TTACGCGTAAAATTCGGGAGCCTTC
BlcC_rev_new TTAGTCTTTCTGCGATCGGTACCC
bclR-rv" GTCTTTCTGCGATCGGTACCC
blcR-CBD-fw AAGCTTGGGTACCGATCGCAGAAAGACCCCGGCGCCAATCCGCCCGGT
pro_Blc_fwd ATGCACCTTCCTTGACACACT
pro_Blc_rev CATCGACCTCTCCCGCATTAG
K516030proB_rev gggccaagtgtgtcaaggaaggtgcatCTCTAGAAGCGGCCGCGAATTCC
K516030proB_fwd tgaactctaatgcgggagaggtcgATGGCTTCCTCCGAAGACGTTAT
BlcB_mut_fwd CCTTTCGAGTTCCGCACCGTTCCCT
BlcB_mut_rev" AGGGAACGGTGCGGAACTCGAAAGG
BlcR-L-sfGFP-rv TCGCCTTTACGCATGCCGGTGTCTTTCTGCGATCGGTACCC
BlcR-T7-RBS-fw TAGAGAAAGAGGAGAAATACTAGATGGGCCAAAG
K60_BlcPshort_r GAGTGAACTATGGCTCTAGTAGCTAGCACAATACC
K60_BlcPshort_f" GCTAGCTACTAGAGCCATAGTTCACTCTAATGATTC
bclR-rv GTCTTTCTGCGATCGGTACCC
pSB1C3blcOnewfw AAGAAGGCTCCCGAATTTTACGCGTAATACTAGTAGCGGCCGCTGCAG
Pblc_UTR_fwd TCAAGTTCAATTCGTTGCACTCTAATGAATAATTTTGTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATC
Pblc_UTR_rev ATTGAACTTGAATCATTAGAGTGAACTCCCTATAGTGAGTCGTATTACTCTAG
BlcR-His_fwd CGTACTACCATCACCATCACCATCACGGCGCCATGGGCCAAAGGGGCC
BlcR-His_rev CCGTGATGGTGATGGTGATGGTAGTACGACATCTAGTATTTCTCCTCTTTCTCTAGTA
gadR-qRT-fw TCAATCCCCTGTAACGG
gadR-qRT-rev CCAGGCGCACCGAACAAGAC
gadT-qRT-fw ATGAGTCAAACAACAGCAAGCATC
gadT-qRT-rev CTCAACCACCTTCGGATG
gBlocks
GABA_B_pSB1C3_f ataagaaaataccgacagggggatattcacTACTAGTAGCGGCCGCTGC
GABA_B_pSB1C3_r cataaaaaaatcccagtcacaggtgattgaCTCTAGAAGCGGCCGCGA
GABA_R_pSB1C3_f tagttttcaaccctttcagcggaggagccatcgTACTAGTAGCGGCCGCTGC
GABA_R_pSB1C3_r ctgtggattaacacaccacctactttttaaCTCTAGAAGCGGCCGCGA
Trp_sy_pSB1C3_f aaagaagttcatcatcaccatcaccactaaTACTAGTAGCGGCCGCTGC
Trp_sy_pSB1C3_r tctcctctttccctatagtgagtcgtattaCTCTAGAAGCGGCCGCGA
PRIA
T7-RBS-arsR-fw AGGGAGATACTAGAGAAAGAGGAGAAATACTAGATGTCATTTCTGTTACC
arsR-L-sfGFP-rv CAGCTCTTCGCCTTTACGCATGCCGGTTAACTGCAAATGTTCTTAC
L-sfGFP-fw ACCGGCATGCGTAAAGGCGAAGAGCTG
T7-RBS-arsR-rv CTAGTATTTCTCCTCTTTCTCTAGTATC
lacI-sfGFP-fw CCCGACTGGAAAGCGGGCAGACCGGCATGCGTAAAGGCGAAGAGC
lacI-sfGFP-His-rv TAATGGTGATGGTGATGGTGTTTGTACAGTTCATCCATACCATGCGTGATGC
T7-RBS-lacI-rv CTGCCCGCTTTCCAGTCGGGAAACCTGTC
C3-lacI-His-fw AACACCATCACCATCACCATTAATAA
UberHis-arsR-fw CACCATCACCATCACCATTAATAATACTAGAGCCAGGCATCAAA
arsR-UberHis_rv TTATTAATGGTGATGGTGATGGTGTTTGTACAGTTCATCCATACC
BlcR-T7-RBS-fw TAGAGAAAGAGGAGAAATACTAGATGGGCCAAAG
BlcR-L-sfGFP-rv TCGCCTTTACGCATGCCGGTGTCTTTCTGCGATCGGTACCC
arsO-fw ACATTCGTTAAGTCATATATGTTTTTGACTTA
arsO-rv TGTAAGCAATTCAGTATATACAAAAACTGAAT
SuffixR-fw CTGCAGCGGCCGCTACTAGTA
lacO-klein ATTTGCTTTGTGAGCGGATAACAAT
lacO-gross TGTGGAATTGTGAGCGGATAACAATTACG
lacI-CBD-fw GACAGGTTTCCCGACTGGAAAGCGGGCAGCCCGGCGCCAATCCGCCCGGTACTACG
lacI-CBD-Vektor-rv GGACTGCAGCGGCCGCTACTAGTATTATTAAAGACACTGGGAGTAGTACG
EndHis-Vektor-lacI-fw TAATAATACTAGTAGCGGCCGCTGCAGTCC
lacI-rv CTGCCCGCTTTCCAGTCGGGAAACC
arsR-dCBD-fw GGGACAGTAAGAACATTTGCAGTTACCCGGCGCCAATCCGCCCGGTAC
CBD-eHisTerm-rv TATTTGATGCCTGGCTCTAGTATTATTAAAGACACTGGGAGTAGTACG
eHis-dTerm-fw TAATAATACTAGAGCCAGGCATCAAA
arsR-rv TAACTGCAAATGTTCTTACTG
bclR-CBD-fw AAGCTTGGGTACCGATCGCAGAAAGACCCCGGCGCCAATCCGCCCGGTACT
CBD-eGFPTerm-rv ATTTGATGCCTGGCTCTAGTATCATCAAAGACACTGGGAGTAGTACG
eGFP-dTerm-fw TGATGATACTAGAGCCAGGCATCAAAT
bclR-rv GTCTTTCTGCGATCGGTACCC
lacIhis0418_fw GGGCAGCACCATCACCATCACCATTAATAATACTAGTAGCGGCCGCTGC
lacIhis0418_rv TTATTAATGGTGATGGTGATGGTGCTGCCCGCTTTCCAGTCG
arsO-rv_cy3 Cy3-TGTAAGCAATTCAGTATATACAAAAACTGAAT
Split primers
Cm_fwd CGGCATCAGCACCTTGTC
Cm_rev TATACGCAAGGCGACAAG

The gene-specific part of the primer is written in uppercase letters, while lowercase letters denote an overhang.