Difference between revisions of "Team:Minnesota/Safety"

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In order to comply with state and federal law, all team members that worked in the lab took the following courses: Introduction to Research Safety, Chemical Safety, Chemical Waste Management, and Globally Harmonized System of Classification and Labelling of Chemicals. The two microorganisms we used were CEN.PK2 cells of <i>Saccharomyces cerevisiae</i> and JM109 cells of <i>Escherichia coli</i>. Both of these chassis are biosafety level 1 organisms in addition the former is an auxotrophic strain. We choose to work with yeast because of its general public acceptance. Any disposable surface that touched these organisms or DNA was autoclaved before it was thrown out. Benchtop was wiped down with 70% ethanol.  
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In order to comply with state and federal law, all team members that worked in the lab took the following courses: Introduction to Research Safety, Chemical Safety, Chemical Waste Management, and Globally Harmonized System of Classification and Labelling of Chemicals. The two microorganisms we used were CEN.PK2 cells of <i>Saccharomyces cerevisiae</i> and JM109 cells of <i>Escherichia coli</i>. Both of these chassis are biosafety level 1 organisms in addition the former is an auxotrophic strain. We choose to have yeast as our chassis because of its general public acceptance and for its ability to homolo. Any disposable surface that touched these organisms or DNA was autoclaved before it was thrown out. Benchtop was wiped down with 70% ethanol.  
  
  

Revision as of 00:39, 3 September 2015



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Safety in iGEM

In order to comply with state and federal law, all team members that worked in the lab took the following courses: Introduction to Research Safety, Chemical Safety, Chemical Waste Management, and Globally Harmonized System of Classification and Labelling of Chemicals. The two microorganisms we used were CEN.PK2 cells of Saccharomyces cerevisiae and JM109 cells of Escherichia coli. Both of these chassis are biosafety level 1 organisms in addition the former is an auxotrophic strain. We choose to have yeast as our chassis because of its general public acceptance and for its ability to homolo. Any disposable surface that touched these organisms or DNA was autoclaved before it was thrown out. Benchtop was wiped down with 70% ethanol.

Please visit the main Safety page to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.

On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can go beyond the questions on the safety forms, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)

Safe Project Design

Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:

  • Choosing a non-pathogenic chassis
  • Choosing parts that will not harm humans / animals / plants
  • Substituting safer materials for dangerous materials in a proof-of-concept experiment
  • Including an "induced lethality" or "kill-switch" device

Safe Lab Work

What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!

Safe Shipment

Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?