Difference between revisions of "Team:China Tongji/Achivement"
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Revision as of 09:31, 3 September 2015
Achivement
1. Parts
2. Equipment
3. Modeling
4. Judging Form
1. Parts
Cells sense the environment, process information, and make response to stimuli. To make cells work well in complex natural environments, lots of processes have to be preset to react to various signals. However, when well-characterized modules are combined to construct higher order systems, unpredictable behaviors often occur because of the interplay between modules. Another significant problem is that complex integrated systems composed of numerous parts may cause cell overload.
Figure 1. Schematic demonstration of HIV
We proposed an elegant method to design higher order systems. Instead of merely combining different functional modules, we constructed one integrated processing module with fewer parts by utilizing the common structures between modules. The circuit we designed is a rewirable one and the topological structure of the processing module can be altered to adapt to environmental change. The basic idea is to rewire the connections between parts and devices to implement multiple functions with the help of the site-specific recombination systems.
Our design approach may lead to a revolutionary step towards system integration in synthetic biology. Potential fields of application include organism development, living therapeutics and environment improvement.
2. Equipment
2.1 Install our LED light souce
ACL2520-A
B1CM
B4CM
C4W
DC2100
DMLP550R
FFM1
FL460-10
FL532-10
FL560-10
FL635-10
LEDD1B
M470L3
M530L3
M590L3
M625L3
SM1A14
SM1CP2
SM1L03
SM1T2
SM1V05
TPS001
2.2 The installation of light source
(1) Put the proper light filter into SM1L03 and fix it by SM1RR, and then fix the SM1L03 containing its filter to its light source. After that fix ACL2520-A inside of SM1V05, then fix them in front of SM1L03. Link this packaged light source to the C4W cube by using SM1T2.
(2) Fix FFM1 to the B4C/M by using the prepared screws, then use FFM1 to nip the DMLP550R (please pay attention to the position which the filter located, the light whose wavelength is larger than 550nm will pass this filter, otherwise the light will be reflexed. When choosing the location of light sources, this matter should be considered as well). Then fixed the B4C/M to the cube carefully.
(3) The surface which fixed with B4C/M is the underside of the cube. Use B1C/M to seal up the top surface of the cube. Fix two proper light sources to the surfaces which lights come in of C4W. The surface which light comes out will links to the microscope by using SM1T2 and SM1A14. The remaining surface will blocked by SM1CP2.
(4) The installation has been completed.
Figure1
Figure2
Figure3
2.3 The adjustment of light path
Figure4
(1) The light path will be collimated by changing the length between light source and convex lens. When the length is changing, pay attention to the light spot on the optical screen. If the spot is clear and convergent, it means the light path has been collimated.
(2) After join the light sources to the C4W cube, the light spot can be changed by rotating B4C/M and the screws on B4C/M.
(3) The adjustment has been completed.
2.4 The refit of the light sources
(1) Take down the LED of the light sources by using welding gun. Replace them by the high power LED. Make sure the surfaces of the substrate and the radiator are parallel.
(2) The refit has been completed.
Figure5
Figure6
Figure7
3. Modeling
Cells sense the environment, process information, and make response to stimuli. To make cells work well in complex natural environments, lots of processes have to be preset to react to various signals. However, when well-characterized modules are combined to construct higher order systems, unpredictable behaviors often occur because of the interplay between modules. Another significant problem is that complex integrated systems composed of numerous parts may cause cell overload.
Figure 2. China Tongji logo
Our design approach may lead to a revolutionary step towards system integration in synthetic biology. Potential fields of application include organism development, living therapeutics and environment improvement.
4. Judging Form
Cells sense the environment, process information, and make response to stimuli. To make cells work well in complex natural environments, lots of processes have to be preset to react to various signals. However, when well-characterized modules are combined to construct higher order systems, unpredictable behaviors often occur because of the interplay between modules. Another significant problem is that complex integrated systems composed of numerous parts may cause cell overload.
Cells sense the environment, process information, and make response to stimuli. To make cells work well in complex natural environments, lots of processes have to be preset to react to various signals. However, when well-characterized modules are combined to construct higher order systems, unpredictable behaviors often occur because of the interplay between modules. Another significant problem is that complex integrated systems composed of numerous parts may cause cell overload.
Figure 2. China_Tongji_iGEM_logo
Our design approach may lead to a revolutionary step towards system integration in synthetic biology. Potential fields of application include organism development, living therapeutics and environment improvement.
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