Difference between revisions of "Team:ETH Zurich/Chip"
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<p>One of the biggest challenges of circulating tumor cells is their scarcity in the blood of patients. To overcome this problem, our first idea was to develop a microfluidic chip in order to perform single cell analysis. The biggest advantage of using a microfluidic chip is its ability to perform high-throughput cell biology. | <p>One of the biggest challenges of circulating tumor cells is their scarcity in the blood of patients. To overcome this problem, our first idea was to develop a microfluidic chip in order to perform single cell analysis. The biggest advantage of using a microfluidic chip is its ability to perform high-throughput cell biology. | ||
| − | In order to do so, we wanted to produce water-in-oil emulsion droplets, that can then be sorted by a machine analogous to FACS, (inspired from | + | In order to do so, we wanted to produce water-in-oil emulsion droplets, that can then be sorted by a machine analogous to FACS, (inspired from [<a href="https://2015.igem.org/Team:ETH_Zurich/References#Chiu2015">Chiu 2015</a>)]. In the droplets, a mixture of bacteria and mammalian cells would be present. And the bacteria would express the green fluorescent protein only in the presence of cancer cells, exhibiting both increased lactate production rate and sensitivity to sTRAIL. </p> |
<h2>First Design</h2> | <h2>First Design</h2> | ||
<p> However due to the complexity of this setup, we decided to first explore another design consisting of valves and chambers. Instead of having droplets to isolate single cells, we wanted to have a two-layer microfluidic chip. One of the layer would have been the flow of cells and the other layer, valves controlled by pressure that are able to close the chambers. </p> | <p> However due to the complexity of this setup, we decided to first explore another design consisting of valves and chambers. Instead of having droplets to isolate single cells, we wanted to have a two-layer microfluidic chip. One of the layer would have been the flow of cells and the other layer, valves controlled by pressure that are able to close the chambers. </p> | ||
Revision as of 09:07, 5 September 2015
- Project
- Modeling
- Lab
- Human
Practices - Parts
- About Us
Chip Design
Introduction
One of the biggest challenges of circulating tumor cells is their scarcity in the blood of patients. To overcome this problem, our first idea was to develop a microfluidic chip in order to perform single cell analysis. The biggest advantage of using a microfluidic chip is its ability to perform high-throughput cell biology. In order to do so, we wanted to produce water-in-oil emulsion droplets, that can then be sorted by a machine analogous to FACS, (inspired from [Chiu 2015)]. In the droplets, a mixture of bacteria and mammalian cells would be present. And the bacteria would express the green fluorescent protein only in the presence of cancer cells, exhibiting both increased lactate production rate and sensitivity to sTRAIL.
First Design
However due to the complexity of this setup, we decided to first explore another design consisting of valves and chambers. Instead of having droplets to isolate single cells, we wanted to have a two-layer microfluidic chip. One of the layer would have been the flow of cells and the other layer, valves controlled by pressure that are able to close the chambers.