Difference between revisions of "Team:Nagahama/Experiments"
(→Agarose gel) |
(→Protocols) |
||
Line 22: | Line 22: | ||
=== LB medium (100 mL liquid) === | === LB medium (100 mL liquid) === | ||
+ | |||
+ | 1.Measure 1g Tripton | ||
+ | |||
+ | 2.Measure 0.5g Yeast Extract | ||
+ | |||
+ | 3.Measure 1g Nacl | ||
+ | |||
+ | 4.Add 100mL H2O | ||
+ | |||
+ | 5.autoclave(121℃ 20min) | ||
=== Genome extraction === | === Genome extraction === | ||
=== Plasmids extraction === | === Plasmids extraction === |
Revision as of 04:08, 31 May 2015
Contents
Protocols
Our Lab's Protocols
Agarose gel(100mL)
Method of Making 0.7% Agarose gel
1.Measure 0.7g Agarose
2.Add 100mL H2O
3.Heat(till agarose melted)
4.Pur agarose into a gel maker
5.Set a comb
6.Wait till agarose curdles
7.Pull an comb
LB medium (100 mL liquid)
1.Measure 1g Tripton
2.Measure 0.5g Yeast Extract
3.Measure 1g Nacl
4.Add 100mL H2O
5.autoclave(121℃ 20min)
Genome extraction
Plasmids extraction
Experiments
Experiments & Protocols
Describe the experiments, research and protocols you used in your iGEM project.
What should this page contain?
- Protocols
- Experiments
- Documentation of the development of your project