Difference between revisions of "Team:China Tongji/Notebook"

1.1.1.1 Week1 -- June 6

June 6

1, Design the PCR primers of chR2-YFP.

1.1.1.2 Week2 -- June 8~14

June 8

1,Theamplification of CHR2-YFP (use taq PCR protocol)

2, AGE ( agarose gel electrophoresis )

3, Gel extraction of chR2-YFP.

4, Transformation of GFP, YFP, mcherry in E•coli DH5α.

June 9

1, Select a single clone of each plate. (GFP, YFP, mcherry) .Put the E.coli in 4ml LB buffer and train for one night at 37℃.

2, Transformationof vector with pmyo-3(ppd95.77)

1.1.1.3 Week3 -- June 15~21

1.1.1.4 Week4 -- June 22~25

1.1.2.1 Week2 -- July 11~14

1.1.2.2 Week3 -- July 15~21

1.1.2.3 Week4 -- July 21~27

1.1.2.4 Week5 -- July 28~31

1.1.3.1 Week1 -- August 1~7

1.1.3.2 Week2 -- August 8~13

1.1.3.3 Week3 -- August 15~21

1.1.3.4 Week4 -- August 22~28

1.1.3.5 Week5 -- August 29~31

1.1.4.1 Week1 -- September 1~7

1.1.4.2 Week2 -- September 9~10

1.2.1.1 June 20~29

1.2.2.1 Week2 -- July 9~13

1.2.2.2 Week3 -- July 15~21

1.2.2.3 Week4 -- July 23~28

1.2.2.4 Week5 -- July 29~31

1.2.3.1 Week1 -- August 1~6

1.2.3.2 Week2 -- August 8~14

1.2.3.3 Week3 -- August 15~21

1.2.3.4 Week4 -- August 22~27

1.2.3.5 Week5 -- August 29~31

1.2.4.1 Week1 -- September 1~7

1.2.4.2 Week2 -- September 9~15

1.3.1.1 Week1 -- August 3~7

1.3.1.2 Week2 -- August 9~14

1.3.1.3 Week3 -- August 15~21

1.3.1.4 Week4 -- August 22~28

1.3.1.5 Week5 -- August 30

1.3.2.1 Week1 -- September 1~7

1.3.2.2 Week2 -- September 8~16

1.4.1.1 Week1 -- July 1

1.4.1.2 Week4 -- July 28

1.4.2.1 Week1 -- August 1~7

1.4.2.2 Week2 -- August 8