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Revision as of 22:28, 10 September 2015
- Project
- Modeling
- Lab
- Human
Practices - Parts
- About Us
Basic Parts
In this section we present our basic parts. We expressed human Annexin V in Escherichia coli and then made a phusion protein with Annexin V and the Ice Nucleation Protein (INP). The former was created to allow our bacteria to serve as detectors of apoptosis by specifically binding phosphatidylserine.
Our other part is LldR, a protein required to regulate the lldR promoter. The expression of LldR was necessary to characterize the natural and our designed lldR promoters.
Part |
Content |
Registry number |
| Annexin V | Promoter J23114-RBS B0034- Annexin V optimized for Escherichia coli | BBa_K1847000 |
| INP-Annexin V | Promoter J23114-RBS B0034- INP-Annexin V fusion protein | BBa_K1847015 |
| LldR | Promoter J23118-RBS B0034-lldR | BBa_K1847001 |
