Difference between revisions of "Team:ETH Zurich/Basic Part"

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<h1> Basic Parts</h1>
 
<h1> Basic Parts</h1>
 
<p>In this section we present our basic parts. We expressed <a href="https://2015.igem.org/Team:ETH_Zurich/Results#Bacterial_sensor">human Annexin V</a> in <i>Escherichia coli</i> and then made a phusion protein with <a href="https://2015.igem.org/Team:ETH_Zurich/Results#Bacterial_sensor">Annexin V and the Ice Nucleation Protein (INP)</a>. The former was created to allow our bacteria to serve as detectors of apoptosis by specifically binding phosphatidylserine. </p>
 
<p>In this section we present our basic parts. We expressed <a href="https://2015.igem.org/Team:ETH_Zurich/Results#Bacterial_sensor">human Annexin V</a> in <i>Escherichia coli</i> and then made a phusion protein with <a href="https://2015.igem.org/Team:ETH_Zurich/Results#Bacterial_sensor">Annexin V and the Ice Nucleation Protein (INP)</a>. The former was created to allow our bacteria to serve as detectors of apoptosis by specifically binding phosphatidylserine. </p>
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Revision as of 22:29, 10 September 2015

"What I cannot create I do not understand."
- Richard Feynmann

Basic Parts

In this section we present our basic parts. We expressed human Annexin V in Escherichia coli and then made a phusion protein with Annexin V and the Ice Nucleation Protein (INP). The former was created to allow our bacteria to serve as detectors of apoptosis by specifically binding phosphatidylserine.

Our other part is LldR, a protein required to regulate the lldR promoter. The expression of LldR was necessary to characterize the natural and our designed lldR promoters.

Part

Content

Registry number
Annexin V Promoter J23114-RBS B0034- Annexin V optimized for Escherichia coli BBa_K1847000
INP-Annexin V Promoter J23114-RBS B0034- INP-Annexin V fusion protein BBa_K1847015
LldR Promoter J23118-RBS B0034-lldR BBa_K1847001

We would like to thank our sponsors