Difference between revisions of "Team:Gifu/InterLab"

Line 143: Line 143:
 
<li><a href="https://2015.igem.org/Team:Gifu/InterLab" style="text-decoration:none;" style="text-decoration:none;" >InterLab Study&nbsp;&nbsp;&nbsp;</a></li>
 
<li><a href="https://2015.igem.org/Team:Gifu/InterLab" style="text-decoration:none;" style="text-decoration:none;" >InterLab Study&nbsp;&nbsp;&nbsp;</a></li>
 
<li><a href="https://2015.igem.org/Team:Gifu/Safety"style="text-decoration:none;" > SAFETY&nbsp; </a></li>
 
<li><a href="https://2015.igem.org/Team:Gifu/Safety"style="text-decoration:none;" > SAFETY&nbsp; </a></li>
−
<li><a href="#" style="text-decoration:none;" > &nbsp; </a></li>
+
<li><a href="https://2015.igem.org/Team:Gifu/human-practice" style="text-decoration:none;" >Human Practice &nbsp; </a></li>
 +
 
 
<li><a href="#" style="text-decoration:none;" > &nbsp; </a></li>
 
<li><a href="#" style="text-decoration:none;" > &nbsp; </a></li>
 
<li><a href="#" style="text-decoration:none;" > &nbsp; </a></li>
 
<li><a href="#" style="text-decoration:none;" > &nbsp; </a></li>

Revision as of 00:59, 12 September 2015


https://static.igem.org/mediawiki/2015/d/d8/Logo_HOME.png https://static.igem.org/mediawiki/2015/a/a4/Logo_PROJECT.png https://static.igem.org/mediawiki/2015/archive/b/b9/20150720104417%21Logo_TEAM.png https://static.igem.org/mediawiki/2015/8/86/Logo_NOTE.png https://static.igem.org/mediawiki/2015/8/87/Logo_RESULT.png https://static.igem.org/mediawiki/2015/2/28/Logo_MORE.png


Inter Lab Study


Equipment

  • BioSHAKER TA-12R
    • use for shaking culture



  • Safire ART-NR:F129013
    • use for measurement of absorbance




Method of Measurement

      We constructed 5 plasmids [positive control, negative control, device1~3] by using BioBricks and then transformed to E. coli K-12 JM109. We checked them by colony direct PCR and plasmids sequence. We cultivated correct bacterias by shaking until OD660:0.5. Cultivated mediums were　transferred to 96-well plate by 100mL. We read the absorbance.


      Setting of plate reader is following:



Result

  • Definition

    •   We expressed absorbance as relative value for　positive control.
    This expression is following:
      Sample : Absorbance in sample
      NCav : Average of absorbance in negative control
      PCav : Average of absorbance in positive control



  • Value

    • Relative absorbance values for positive control are shown below.



    FIG1 Relative absorbance values
    20A : Device1 (J23101 + I13504)
    22A : Device2 (J23106 + I13504)
    22K : Device3 (J23117 + I13504)




Discussion

      The value of device1 is much higher than that of device2 and 3. This is why each device is different from promoter sequence, especially -10 and -35 region.



    Promoter sequence in device1 resembles consensus sequence. On the other hand, device2 and 3 is not similar than device1. This is how each GFP expression is different.









  MENU
  1. InterLab Study   
  2. SAFETY 
  3. Human Practice  
  4.  
  5.  
  6.  
  7.  
  8.  
  9.