Difference between revisions of "Team:Technion HS Israel/Notebook"
(Prototype team page) |
|||
Line 1: | Line 1: | ||
− | {{ | + | {{Technion_HS_Israel4}} |
<html> | <html> | ||
+ | <link href="//2015.igem.org/Template:Technion_HS_Israel4/Technion_HS_Israel_menu_style?action=raw&ctype=text/css" rel="stylesheet"> | ||
+ | <h1>Notebook</h1> | ||
+ | <p> Here is a summery of our notebook. For the full notebook please look at the following scans</p> | ||
+ | <table class="NewTable" border="1"> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th>Week</th> | ||
+ | <th>Design</th> | ||
+ | <th>Cloning</th> | ||
+ | <th>Experiment</th> | ||
+ | <th>Finish new BioBrick</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tr> | ||
+ | <td>Jul 5th to Jul 9th (2015)</td> | ||
+ | <td>Primers : OGEM 1 and 2 to open j09855 plasmid . <br></br>Gblock :RBS- Aiia with a Gibson overlabs.</td> | ||
+ | <td>we got j09855 part from the distribution kit.</td> | ||
+ | <td>-</td> | ||
+ | <td>-</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>June 12th to July 16th (2015)</td> | ||
+ | <td>Primers : OGEM 3 and 4 to got the k1767001 plasmid without LuxR , after doing the PCR we will get a linear DNA so we put two enzymes in the overlaps of the primers to close the plasmids. </td> | ||
+ | <td>PCR to open the j09855 plasmid.Gibson to add Aiia .</td> | ||
+ | <td>-</td> | ||
+ | <td>BBa_K1767001</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Aug. 2nd to Aug. 6th (2015)</td> | ||
+ | <td>-</td> | ||
+ | <td>We got p0440 part from the distribution kit.<br></br>We built BBa_K1767002 and BBa_K1767004 Constructs by restriction enzymes. <br></br>PCR to get the k1767001 plasmid without LuxR .</td> | ||
+ | <td>-</td> | ||
+ | <td>BBa_K1767002 <br></br>BBa_K1767004</td> | ||
+ | </tr> | ||
− | < | + | <tr> |
+ | <td>Aug. 9th to Aug. 13th (2015)</td> | ||
+ | <td>Primers : OGEM 5 and 6 to check if we succeed in the PCR ( Those primers for colony PCR)</td> | ||
+ | <td>We got I13521 part from the distribution kit.</br> We built BBa_K1767003 and BBa_K1767005 Constructs by restriction enzymes. </br>Colony PCR to check if we have LuxR in the plasmid.</td> | ||
+ | <td>-</td> | ||
+ | <td>BBa_K1767003 </br>BBa_K1767005</td> | ||
+ | </tr> | ||
− | < | + | <tr> |
− | + | <td>Aug. 16th to Aug. 20th (2015)</td> | |
− | < | + | <td>-</td> |
− | < | + | <td>-</td> |
− | < | + | <td>AHL experiment #1</td> |
− | < | + | <td>-</td> |
− | < | + | </tr> |
− | < | + | <tr> |
− | </ | + | <td>Aug. 23rd to Aug. 27th (2015)</td> |
− | + | <td>Primers : OGEM 7 and 8 to check if we have yfp in the plasmid ( for colony PCR ).</td> | |
− | + | <td>We got I13003 part from the distribution kit.</td> | |
− | < | + | <td>AHL experiment #2</td> |
− | < | + | <td>-</td> |
− | + | </tr> | |
− | < | + | <tr> |
− | < | + | <td>Aug. 30th to Sept. 9th (2015)</td> |
− | < | + | <td>Primers: OGEM 9 and 10 to replaced the RFP with RFP ( with LVA )</td> |
− | < | + | <td>We built BBa_K1767009 and BBa_K1767010 Constructs by restriction enzymes.</br>Colony PCR to check if we have YFP in the plasmid.</td> |
− | < | + | <td>-</td> |
− | </ | + | <td>BBa_K1767009 </br>BBa_K1767010 |
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Sept. 6th to Sept. 11th (2015)</td> | ||
+ | <td>-</td> | ||
+ | <td>-</td> | ||
+ | <td>AHL experiment # 3</td> | ||
+ | <td>-</td> | ||
+ | </tr> | ||
+ | </table> | ||
</div> | </div> | ||
</html> | </html> |
Revision as of 09:22, 12 September 2015
Notebook
Here is a summery of our notebook. For the full notebook please look at the following scans
Week | Design | Cloning | Experiment | Finish new BioBrick |
---|---|---|---|---|
Jul 5th to Jul 9th (2015) | Primers : OGEM 1 and 2 to open j09855 plasmid . Gblock :RBS- Aiia with a Gibson overlabs. |
we got j09855 part from the distribution kit. | - | - |
June 12th to July 16th (2015) | Primers : OGEM 3 and 4 to got the k1767001 plasmid without LuxR , after doing the PCR we will get a linear DNA so we put two enzymes in the overlaps of the primers to close the plasmids. | PCR to open the j09855 plasmid.Gibson to add Aiia . | - | BBa_K1767001 |
Aug. 2nd to Aug. 6th (2015) | - | We got p0440 part from the distribution kit. We built BBa_K1767002 and BBa_K1767004 Constructs by restriction enzymes. PCR to get the k1767001 plasmid without LuxR . |
- | BBa_K1767002 BBa_K1767004 |
Aug. 9th to Aug. 13th (2015) | Primers : OGEM 5 and 6 to check if we succeed in the PCR ( Those primers for colony PCR) | We got I13521 part from the distribution kit. We built BBa_K1767003 and BBa_K1767005 Constructs by restriction enzymes. Colony PCR to check if we have LuxR in the plasmid. | - | BBa_K1767003 BBa_K1767005 |
Aug. 16th to Aug. 20th (2015) | - | - | AHL experiment #1 | - |
Aug. 23rd to Aug. 27th (2015) | Primers : OGEM 7 and 8 to check if we have yfp in the plasmid ( for colony PCR ). | We got I13003 part from the distribution kit. | AHL experiment #2 | - |
Aug. 30th to Sept. 9th (2015) | Primers: OGEM 9 and 10 to replaced the RFP with RFP ( with LVA ) | We built BBa_K1767009 and BBa_K1767010 Constructs by restriction enzymes.Colony PCR to check if we have YFP in the plasmid. | - | BBa_K1767009 BBa_K1767010 |
Sept. 6th to Sept. 11th (2015) | - | - | AHL experiment # 3 | - |