Difference between revisions of "Team:Hong Kong-CUHK/protocols"

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<h3> (1) Bacterial DNA extraction protocol for Azotobacter vinelandii or E.coli
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<p> - We are using the <a href="http://www.takara.co.kr/file/manual/pdf/9763_e.v1309Da.pdf">TaKaRa MiniBest Bacteria Genomic DNA Extraction Kit</a> of Takara according to the manufacurer directions.</p>
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<h3> (2) MiniPrep </h3>
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<p>- We are using the <a href="http://eshop.intronbio.com/product/detail04.asp?pIdx=1">DNA-spin™ Plasmid DNA Purification Kit of Intron Technology according to the manufacturer directions. </p>
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<h3> (3) Preparation of chemically competent BL21 E.coli cells </h3>
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<h4> Day 1 </h4>
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<center> - Streak BL21 on a LB agar plate without antibiotic, grow overnight in 37℃ incubator </center>

Revision as of 15:44, 12 September 2015