contents

1. Introduction

2. Summary of the Experiment

3. Results

4. Discussion

5. Materials and Methods

5-1. Strains

5-2. Plasmid

5-3. Assay Protocol

6. Reference

1. Introduction

　　　　　　

In iGEM 2015, the Interlab Study was held, where we measured the expression level of GFP using three designated devices. It was the first time for our team to join this Interlab Study. In addition to the three designated devices, we also measured the expression level of GFP from a positive control and a negative control using the flow cytometer and the plate reader. Also, for the plate reader, we succeeded in calculating the absolute unit by drawing the calibration curve using sodium fluorescein.

2. Summary of the Experiment

　　　　　　

Our purpose was to obtain the fluorescence data of the three designated devices and to compare them. We prepared Device1〜Device3, Positive control and Negative control as shown below. We measured the exact same colonies of the exact same samples with both the plate reader and the flow cytometer.

Device 1: J23101 + I13504(pSB1C3)

Device 2: J23106 + I13504(pSB1C3)

Device 3: J23117 + I13504(pSB1C3)

Positive control: BBa_I20270(pSB1C3)

Negative control: BBa_R0040(pSB1C3)

Fig3-7-2-1 designated devices

3. Results

　　　　　　　まだまだ書ける。

4. Discussion

　　　　　　　まだまだいける。

5. Materials and Methods

5-1. Strains

5-2. Plasmids

5-3. Assay Protocol

6. Reference

　　　　　　　めでたし、めでたし。