Difference between revisions of "Team:SPSingapore/Notebook-Week-2"

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   <li class = 'active'><a href='https://2015.igem.org/Team:SPSingapore/Notebook'><span>Notebook</span></a></li>
 
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Revision as of 18:37, 12 September 2015


Research Notebook

Week 2 (31/5 - 6/6)

31st May 2015

Xin Yi

• Sent EsaR clone 4 and INv-4 for sequencing.

2nd June 2015

Yi Han

• YFP and GFP transformation results - no colonies for YFP
• The GFP transformation repeated with 100ng of plasmid was sucessful.
• 4 colonies of gfp plasmid were inoculated in 3mL LB+amp and grown overnight.

3rd June 2015

Yi Han

• Miniprep of gfp plasmids
• RE digest with EcoRI and RsaI

4th June 2015

Chi Yan

• RE digest indicated a very faint smaller band for colonies 2-4, and hence these were likely to be positive clones

5th June 2015

Yan Ting

• Inoculated 3mLS of Inv-4 and EsaR-4 plasmid carrying bacteria into 100mL LB+ appropriate Antibiotic
• Cell culture → HEK293 cells revived from freezing down appeared detached.

6th June 2015

Yi Han & Yan Ting

• Storage of bacterial glycerol stocks for Inv-4, EsaR4 in 25% glycerol
• Yan Ting: grew HEK293T cells in T25 glask
• Gel extract to clean up gfp plasmids which loading dye had accidentally been added to.