Difference between revisions of "Team:Tokyo Tech/Experiment/Interlab"
| Line 27: | Line 27: | ||
<h3 class="link"><a href="#Summary">2. Summary of the Experiment</a></h3> | <h3 class="link"><a href="#Summary">2. Summary of the Experiment</a></h3> | ||
<h3 class="link"><a href="#Results">3. Results</a></h3> | <h3 class="link"><a href="#Results">3. Results</a></h3> | ||
| + | <h3 class="link2"><a href="#Plate">3-1. Plate reader</a></h3> | ||
| + | <h3 class="link2"><a href="#Plasmids">3-2. Plasmid</a></h3> | ||
| + | <h3 class="link2"><a href="#Assay">5-3. Assay Protocol</a></h3> | ||
<h3 class="link"><a href="#Discussion">4. Discussion</a></h3> | <h3 class="link"><a href="#Discussion">4. Discussion</a></h3> | ||
<h3 class="link"><a href="#Materials">5. Materials and Methods</a></h3> | <h3 class="link"><a href="#Materials">5. Materials and Methods</a></h3> | ||
| Line 64: | Line 67: | ||
<h2 id="Results" class="smalltitle">3. Results</h2> | <h2 id="Results" class="smalltitle">3. Results</h2> | ||
| − | + | <h3 id="Plate" class="sub5">3-1. Plate reader</h3> | |
<h2 id="Discussion" class="smalltitle">4. Discussion</h2> | <h2 id="Discussion" class="smalltitle">4. Discussion</h2> | ||
まだまだいける。 | まだまだいける。 | ||
Revision as of 18:43, 12 September 2015
Interlab
We have measured three devices.
contents
1. Introduction
2. Summary of the Experiment
3. Results
3-1. Plate reader
3-2. Plasmid
5-3. Assay Protocol
4. Discussion
5. Materials and Methods
5-1. Strains
5-2. Plasmid
5-3. Assay Protocol
6. Reference
1. Introduction
In iGEM 2015, the Interlab Study was held, where we measured the expression level of GFP using three designated devices. It was the first time for our team to join this Interlab Study. In addition to the three designated devices, we also measured the expression level of GFP from a positive control and a negative control using the flow cytometer and the plate reader. Also, for the plate reader, we succeeded in calculating the absolute unit by drawing the calibration curve using sodium fluorescein.
2. Summary of the Experiment
Our purpose was to obtain the fluorescence data of the three designated devices and to compare them. We prepared Device1〜Device3, Positive control and Negative control as shown below. We measured the exact same colonies of the exact same samples with both the plate reader and the flow cytometer.
Device 1: J23101 + I13504(pSB1C3)
Device 2: J23106 + I13504(pSB1C3)
Device 3: J23117 + I13504(pSB1C3)
Positive control: BBa_I20270(pSB1C3)
Negative control: BBa_R0040(pSB1C3)
|
|
Fig3-7-2-1. designated devices |
3. Results
3-1. Plate reader
4. Discussion
まだまだいける。5. Materials and Methods
5-1. Strains
5-2. Plasmids
5-3. Assay Protocol
6. Reference
めでたし、めでたし。