Difference between revisions of "Team:Gifu/Safety"

 
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<center><font size="20" face="Century">Inter Lab Study</font></center><br><br>
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<center><font size="20" face="Century">SAFETY</font></center><br><br>
  
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<font size="6" face="Arimo">Equipment</font>  
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<font size="6" face="Century">Final Safety Form</font>  
 
<hr width="100%" ><br>
 
<hr width="100%" ><br>
 
<ul>
 
<ul>
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<li> <font size="5" face="Arimo"> BioSHAKER TA-12R<br></font> </li>
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<li> <font size="5" face="Century"> What is your chassis organism?<br></font> </li>
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<font size="4" face="Arimo">
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<font size="4" face="Century">
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use for shaking culture</font>
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<p><i>E. coli</i> K-12 JM109</p>
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  <br><br><br><br>
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</font>
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<li> <font size="5" face="Arimo"> Safire ART-NR:F129013<br></font> </li>
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<br><br>
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<font size="4" face="Arimo">
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use for measurement of absorbance</font>
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<li> <font size="5" face="Century"> Do you plan to experiment with any other organisms, besides your chassis? <br></font> </li>
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<font size="4" face="Century">
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<p>No.  </p>
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</font>
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  <br><br>
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<li> <font size="5" face="Century"> How will your project work? <br></font> </li>
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<font size="4" face="Century">
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<p>We aim to create useful long chain protein.  </p>
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</font>
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<br><br>
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<li> <font size="5" face="Century"> What risks does your project pose at the laboratory stage?<br>
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What actions are you taking to reduce those risks?  <br></font> </li>
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<font size="4" face="Century">
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<p>T4 phage may do harm to <i>E.coli</i>. So, we used T4 phage in other room.  </p>
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</font>
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<br><br>
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<li> <font size="5" face="Century"> How would your project be used in the real world? <br></font> </li>
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<font size="4" face="Century">
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<p>Our project is foundational / we do not have a specific real-world application in mind  </p>
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</font>
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<br><br>
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<li> <font size="5" face="Century">What risks might your project pose, if it were fully developed into a real product that real people could use? <br>
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What future work might you do to reduce those risks? <br></font> </li>
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<font size="4" face="Century">
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<p>A toxic protein can be produced using parts we are going to make. Any materials in our lab should not be transferred without a permission from our instructor.  </p>
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</font>
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<br><br>
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<li> <font size="5" face="Century"> Any further comments about your project: <br></font> </li>
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<font size="4" face="Century">
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<p>No.  </p>
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</font>
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<br><br>
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<li> <font size="5" face="Century"> Comments about this form: Is it easy or difficult to use? Are the questions confusing? <br></font> </li>
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<font size="4" face="Century">
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<p>No.  </p>
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</font>
 
  <br><br><br><br><br>
 
  <br><br><br><br><br>
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</ul>
 
  
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<font size="6" face="Arimo">Method of Measurement</font>
 
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<hr width="100%" >
 
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<ul>
 
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<font size="4" face="Arimo">
 
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&nbsp;&nbsp;We constructed 5 plasmids [positive control, negative control, device1~3] by using BioBricks and then transformed to <i>E. coli</i> K-12 JM109. We checked them by colony direct PCR and plasmids sequence. We cultivated correct bacterias by shaking until OD660:0.5. Cultivated mediums were　transferred to 96-well plate by 100mL. We read the absorbance.
 
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<br>
 
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&nbsp;&nbsp;Setting of plate reader is following:<br></font>
 
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<img src="https://static.igem.org/mediawiki/2015/2/20/Set_safari_gify.png" border="0" width="395" height="220">
 
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<br><br><br><br>
 
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</ul>
 
  
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<font size="6" face="Arimo">Result and discussion</font>  
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<font size="6" face="Century">Discussion with our teachers</font>  
 
<hr width="100%" ><br>
 
<hr width="100%" ><br>
 
<ul>
 
<ul>
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<li> <font size="5" face="Arimo"> Definition<br></font> </li>
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<font size="4" face="Century">
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<font size="4" face="Arimo">
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<p> We sometimes discussed our safety with our teachers. I show a state of the discussion in the following video. </p>
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We expressed absorbance as relative value for　positive control. <br>This expression is following:<br></font>
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</font>
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<img src="https://static.igem.org/mediawiki/2015/8/85/Siki-gifu.png" border="0" width="211" height="57"><br>
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  <br><br>
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&nbsp;&nbsp;Sample : Absorbance in sample<br>
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&nbsp;&nbsp;NCav : Average of absorbance in negative control<br>
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&nbsp;&nbsp;PCav : Average of absorbance in positive control<br>
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  <br><br><br>
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<li> <font size="5" face="Arimo"> Value<br></font> </li>
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<font size="4" face="Arimo">
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Relative absorbance values for positive control are shown below. <br>
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<img src="https://static.igem.org/mediawiki/2015/e/e4/GURAHU-gifu.png" border="0" width="572" height="364"> <br><br>
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<iframe width="560" height="315" src="https://www.youtube.com/embed/7U9A4P0VtNg" frameborder="0" allowfullscreen></iframe>
  
 
</ul>
 
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<li><a href="https://2015.igem.org/Team:Gifu/InterLab" style="text-decoration:none;" style="text-decoration:none;" >InterLab Study&nbsp;&nbsp;&nbsp;</a></li>
 
<li><a href="https://2015.igem.org/Team:Gifu/InterLab" style="text-decoration:none;" style="text-decoration:none;" >InterLab Study&nbsp;&nbsp;&nbsp;</a></li>
 
<li><a href="https://2015.igem.org/Team:Gifu/Safety"style="text-decoration:none;" > SAFETY&nbsp; </a></li>
 
<li><a href="https://2015.igem.org/Team:Gifu/Safety"style="text-decoration:none;" > SAFETY&nbsp; </a></li>
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<li><a href="#" style="text-decoration:none;" > &nbsp; </a></li>
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<li><a href="https://2015.igem.org/Team:Gifu/human-practice" style="text-decoration:none;" >Policy & Practice &nbsp; </a></li>
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<li><a href="#" style="text-decoration:none;" > &nbsp; </a></li>
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<br>
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<li><a href="#" style="text-decoration:none;" > &nbsp; </a></li>
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<li><a href="#" style="text-decoration:none;" > &nbsp; </a></li>
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<li><a href="#" style="text-decoration:none;" > &nbsp; </a></li>
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<li><a href="#" style="text-decoration:none;" > &nbsp; </a></li>
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<li><a href="#" style="text-decoration:none;" > &nbsp; </a></li><br>
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</ol>
 
</ol>
  

Latest revision as of 10:59, 13 September 2015


https://static.igem.org/mediawiki/2015/d/d8/Logo_HOME.png https://static.igem.org/mediawiki/2015/a/a4/Logo_PROJECT.png https://static.igem.org/mediawiki/2015/archive/b/b9/20150720104417%21Logo_TEAM.png https://static.igem.org/mediawiki/2015/8/86/Logo_NOTE.png https://static.igem.org/mediawiki/2015/8/87/Logo_RESULT.png https://static.igem.org/mediawiki/2015/2/28/Logo_MORE.png


SAFETY


Final Safety Form

  • What is your chassis organism?

    • E. coli K-12 JM109

  • Do you plan to experiment with any other organisms, besides your chassis?

    • No.



  • How will your project work?

    • We aim to create useful long chain protein.



  • What risks does your project pose at the laboratory stage?
    What actions are you taking to reduce those risks?

    • T4 phage may do harm to E.coli. So, we used T4 phage in other room.



  • How would your project be used in the real world?

    • Our project is foundational / we do not have a specific real-world application in mind



  • What risks might your project pose, if it were fully developed into a real product that real people could use?
    What future work might you do to reduce those risks?

    • A toxic protein can be produced using parts we are going to make. Any materials in our lab should not be transferred without a permission from our instructor.



  • Any further comments about your project:

    • No.



  • Comments about this form: Is it easy or difficult to use? Are the questions confusing?

    • No.






    Discussion with our teachers

      We sometimes discussed our safety with our teachers. I show a state of the discussion in the following video.












  MENU
  1. InterLab Study   
  2. SAFETY 
  3. Policy & Practice