Difference between revisions of "Team:Bielefeld-CeBiTec/Collaborations"
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| + | <h2>Collaboration with team Dundee 2015</h2> | ||
| + | <p> Some part of our project was similar to team Dundee´s. They build a chromium biosensor based on the same system we used, the chromate inducible operon of <i>Ochrobactrum tritici 5bvl1</i>. Therefore we decided to cooperate and exchanged plans and plasmid maps, as well as sequences. Thereby we realized they were comparing the original sequence to one with the first 15 codons optimized, while our system bases on a complete codon optimized repressor. After actually generating our sensors we exchanged samples to test in each other’s systems. And look for differences. We were able to measure a combination of our promoter and their repressor in CFPS. </p> | ||
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Revision as of 12:19, 13 September 2015
Collaborations
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Collaboration with iGEM team from Freiburg
Our team from Bielefeld sent a plasmid based on BBa_I746909 that has a translation enhancing sequence (5’-UTR), and team Freiburg sent a plasmid containing turboYFP, a His and a Halo Tag. We would like to compare if these parts work in different cell free proteins synthesis environments.
Collaboration with team Dundee 2015
Some part of our project was similar to team Dundee´s. They build a chromium biosensor based on the same system we used, the chromate inducible operon of Ochrobactrum tritici 5bvl1. Therefore we decided to cooperate and exchanged plans and plasmid maps, as well as sequences. Thereby we realized they were comparing the original sequence to one with the first 15 codons optimized, while our system bases on a complete codon optimized repressor. After actually generating our sensors we exchanged samples to test in each other’s systems. And look for differences. We were able to measure a combination of our promoter and their repressor in CFPS.