Difference between revisions of "Team:Sherbrooke/Results"

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<h2>Experimentations Results<h2>
 
<h2>Experimentations Results<h2>
 
<hr><hr><hr>
 
<hr><hr><hr>
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</br>
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<h2>Project modules</h2>
 
<h2>Project modules</h2>
 
<ul>
 
<ul>
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<hr><hr>
 
<hr><hr>
 
<p>
 
<p>
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A <a href="#MC96 Thermal Experimentations">thermal experimentation </a>has been the only experimentation done on the <i>MC96</i> module.
+
A <a href="#MC96 Thermal Experimentations Results">thermal experimentation </a>has been the only experimentation done on the <i>MC96</i> module.
 
</p>
 
</p>
  
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<span id="Achievements"> &nbsp; </span>
 
<span id="Achievements"> &nbsp; </span>
−
<h2>Achievements<h2>
+
<h2>Achievements</h2>
 
<hr><hr><hr>
 
<hr><hr><hr>
 
</br>
 
</br>
 +
<p>
 +
The project has been done in the context of a
 +
end of baccalaureate project that is ending in December 2015.
 +
Thus, some specifications that have not been reached for the
 +
IGEM competition could be achieved in the following months.
 +
</p>
 +
<p> <font color="green">Specification achieved</font></p>
 +
<p> <font color="red">Specification not achieved yet</font></p>
  
 +
<font color="#565656">Platform:</font>
 +
<ul>
 +
<li><font color="green">Movements of the robotic platform must have a 1 mm precision</font></li>
 +
<li><font color="green">Complete platform must be fully open-hardware and detailed at no more than 10000$</font></li>
 +
</ul>
 +
 +
<font color="#565656">Tools Holder:</font>
 +
<ul>
 +
<li><font color="red">Must be able to use different kinds of pipette tools at the same time</font></li>
 +
<li><font color="red">Must be able to change tools with ease.</font></li>
 +
</ul>
 +
 +
<font color="#565656">Gripper:</font>
 +
<ul>
 +
<li><font color="red">Have a range of opening from 0 mm to 85 mm</font></li>
 +
<li><font color="red">Must grab as small as 1.5 mL tubes</font></li>
 +
<li><font color="red">Must grab as large as 96-well plates</font></li>
 +
</ul>
 +
 +
<font color="#565656">Centrifuge:</font>
 +
<ul>
 +
<li><font color="red">Rotate at a speed capable of exerting a minimum gravitational force of 6000G</font></li>
 +
<li><font color="red">Must be equipped with security devices such as detection of
 +
abnormal vibration or securing the lid after closing</font></li>
 +
</ul>
 +
 +
<font color="#565656">MC96:</font>
 +
<ul>
 +
<li><font color="green">Suitable for a 96-well plate (common sized plate for biological manipulations</font></li>
 +
<li><font color="red">Control and maintain temperature cycling between 4 to 80&#8451; &#177;1.5&#8451;</font></li>
 +
<li><font color="red">Achieve a cooling and heating ramp of 0.5 to 1&#8451;/s</font></li>
 +
<li><font color="red">Apply an electromagnetic field on demand</font></li>
 +
<li><font color="red">Less than 1000$</font></li>
 +
</ul>
 +
 +
<font color="#565656">MC1.5:</font>
 +
<ul>
 +
<li><font color="green">Suitable for a test tube of 1.5mL</font></li>
 +
<li><font color="green">Capable of independent control for each unit of three tubes</font></li>
 +
<li><font color="green">Control and maintain temperature cycling between 4 to 80&#8451; &#177;1.5&#8451;</font></li>
 +
<li><font color="green">Achieve a cooling and heating ramp of 0.5 to 1&#8451;/s</font></li>
 +
<li><font color="green">Apply an electromagnetic field on demand</font></li>
 +
<li><font color="green">Less than 1000$</font></li>
 +
</ul>
 +
 +
<font color="#565656">MC1.5:</font>
 +
<ul>
 +
<li><font color="green">Suitable for a glass tube having a diameter of 25mm, capacity of 50mL</font></li>
 +
<li><font color="green">Independent control for each tube</font></li>
 +
<li><font color="green">Control and maintain temperature cycling between 0 to 37&#8451; &#177;1.5&#8451;</font></li>
 +
<li><font color="green">Achieve a heating ramp of 0.08&#8451;/s</font></li>
 +
<li><font color="green">Achieve a cooling ramp of 0.1&#8451;/s above room's temperature</font></li>
 +
<li><font color="green">Achieve a cooling ramp of 0.025&#8451;/s below room's temperature</font></li>
 +
<li><font color="green">Stirring the liquid (Mixing of bacterial cultures)</font></li>
 +
<li><font color="green">Calculate the optical density of the liquid with a precision of &#177;5% from a reference turbidimeter</font></li>
 +
<li><font color="green">Less than 1000$</font></li>
 +
</ul>
  
 
<span id="Future Plans"> &nbsp; </span>
 
<span id="Future Plans"> &nbsp; </span>
−
<h2>Future Plans<h2>
+
<h2>Future Plans</h2>
 
<hr><hr><hr>
 
<hr><hr><hr>
 
</br>
 
</br>
 +
  
  

Revision as of 13:41, 13 September 2015

Project Results

Overview

 

Experimentations Results

Project modules

 

MC96

A thermal experimentation has been the only experimentation done on the MC96 module.

 

Thermal experimentations results

Has no prototype has been built yet, the only results available are the ones from simulations.

Simulation Results

Some simulation has been done on earlier designs, but none of the final design, due to the complexity of simulating heat pipes. Thus, these results are not finals and will surely improve with the addition of the heat pipes between the Peltier elements and the 96-well aluminium mold.

The following figures represent the repartition of heat at the beginning and the end of a heating speed test:

MC96 Heating speed test image

The final temperature has been achieved in 70 seconds.

The following figures represent the repartition of heat at the beginning and the end of a cooling speed test:

MC96 Cooling speed test image

The final temperature has been achieved in 135 seconds.

Back to MC96
Back to Experimentations Results
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MC1.5

Thermal and magnetisation experimentations have been conduct to validate the design of the MC1.5 module. These are the results of those experimentations.

 

Thermal experimentation results

Simulation Results

These are the simulation results for the latest design of the MC1.5.

The following figures represent the repartition of heat at the beginning and the end of a heating speed test:

MC1.5 Heating speed simulation image

The final temperature has been achieved in 70 seconds.

The following figures represent the repartition of heat at the beginning and the end of a cooling speed test:

MC1.5 Cooling speed simulation image

The final temperature has been achieved in 135 seconds.


Back to MC1.5  

Thermal Trials Results

 
Maintaining a temperature below room temperature test results

These are the results obtained by following this protocol. This table illustrates the relation between the voltages applied to the Peltier element and the set temperature of the aluminium mold.

MC1.5 Table Calibration cold

Conclusion
  • The MC1.5 can reach the client’s low temperature specification of 4℃
  • The MC1.5 can reach the client’s temperature stability specification of ±1.5℃

Back to Thermal Trials Results  
Maintaining a temperature below room temperature test results

These are the results obtained by following this protocol. This table illustrates the relation between the voltages applied to the Peltier element and the set temperature of the aluminium mold.

MC1.5 Table Calibration HOT

Conclusion
  • The MC1.5 can reach the client’s high temperature specification of 80℃
  • The MC1.5 can reach the client’s temperature stability specification of ±1.5℃

Back to Thermal Trials Results  
Cooling speed test results

These are the results obtained by following this protocol. This figure shows the aluminium mold’s temperature versus time for an applied voltage of 15V.

MC1.5 cooling speed test 15V

This figure shows the aluminium mold’s temperature versus time for an applied voltage of 15.5V.

MC1.5 cooling speed test 15.5V

This figure shows the aluminium mold’s temperature versus time for an applied voltage of 16V.

MC1.5 cooling speed test 16V

Conclusion
  • 15.5V is the optimal voltage to apply to obtain the highest cooling speed
  • The MC1.5 can reach the client’s specification of a cooling speed of 0.5℃/s

Back to Thermal Trials Results  
Heating speed test results

These are the results obtained by following this protocol. This figure shows the aluminium mold’s temperature versus time for an applied voltage of 24V.

MC1.5 heating speed test 24V

Conclusion
  • The MC1.5 can reach the client’s specification of a heating speed of 1℃/s

Back to Thermal Trials Results
Back to Thermal Experimentations Results
Back to MC1.5
Back to top  

Magnetisation experimentation results

Applying an electromagnetic field on the test tube liquid is one of the key functionality of the MC1.5. These are the results of the experimentation done to validate this feature in the MC1.5.

 

Magnetisation Trials Results

 
Magnet attraction test results

These are the results obtained by following this protocol. These figures show the displacement of the magnetic beads when an electromagnetic field applied to the test tube.

Magnet attraction test results

Conclusion
  • The neodymium magnet is enough powerful to attract the magnetic beads within 2 minutes.

Back to Magnetisation Trials Results  
Magnet attraction inside aluminium mold test results

Magnet attraction inside aluminium mold test results

Conclusion

Back to Magnetisation Experimentations
Back to Magnetisation Trials Results
Back to MC1.5
Back to top  

TAC

Thermal and turbidity experimentations have been conduct to validate the design of the TAC module.

 

Thermal experimentation results

Simulation Results

These are the simulation results for the latest design of the TAC.

The following figures represent the repartition of heat at the beginning and the end of a heating speed test:

TAC Heating speed simulation image

The final temperature has been achieved in 70 seconds.

The following figures represent the repartition of heat at the beginning and the end of a cooling speed test:

TAC Cooling speed simulation image

The final temperature has been achieved in 135 seconds.


Back to TAC  

Thermal Trials Results

 
Maintaining a temperature below room temperature test results

These are the results obtained by following this protocol. This table illustrates the relation between the voltages applied to the Peltier element and the set temperature of the aluminium mold.

TAC Table Calibration cold

Conclusion
  • The TAC can reach the client’s low temperature specification of 0℃
  • The TAC can reach the client’s temperature stability specification of ±1.5℃

Back to Thermal Trials Results  
Maintaining a temperature below room temperature test results

These are the results obtained by following this protocol. This table illustrates the relation between the voltages applied to the Peltier element and the set temperature of the aluminium mold.

TAC Table Calibration HOT

Conclusion
  • The TAC can reach the client’s high temperature specification of 37℃
  • The TAC can reach the client’s temperature stability specification of ±1.5℃

Back to Thermal Trials Results  
Cooling speed test results

These are the results obtained by following this protocol. This figure shows the aluminium mold’s temperature versus time for an applied voltage of 15V.

TAC cooling speed test 15V

This figure shows the aluminium mold’s temperature versus time for an applied voltage of 15.5V.

TAC cooling speed test 15.5V

This figure shows the aluminium mold’s temperature versus time for an applied voltage of 16V.

TAC cooling speed test 16V

Conclusion
  • 15.5V is the optimal voltage to apply to obtain the highest cooling speed
  • The TAC can reach the client’s specification of a cooling speed of 0.3℃/s over room temperature.
  • The TAC can reach the client’s specification of a cooling speed of 0.2℃/s below room temperature.

Back to Thermal Trials Results  
Heating speed test results

These are the results obtained by following this protocol. This figure shows the aluminium mold’s temperature versus time for an applied voltage of 24V.

TAC heating speed test 24V

Conclusion
  • The TAC can reach the client’s specification of a heating speed of 1℃/s

Back to Thermal Trials Results
Back to Thermal Experimentations Results
Back to TAC
Back to top  

Turbidity experimentation results

The purpose of this experiment is to calibrate the turbidity function on the TAC.

Results

These are the results obtained by following this protocol. The following figure is the calibration curve obtained by ….

TAC turbidity calibration

Conclusion
  • The TAC is able to give a turbidity measure with ±5% of a reference turbidimeter
 

Achievements


The project has been done in the context of a end of baccalaureate project that is ending in December 2015. Thus, some specifications that have not been reached for the IGEM competition could be achieved in the following months.

Specification achieved

Specification not achieved yet

Platform:
  • Movements of the robotic platform must have a 1 mm precision
  • Complete platform must be fully open-hardware and detailed at no more than 10000$
Tools Holder:
  • Must be able to use different kinds of pipette tools at the same time
  • Must be able to change tools with ease.
Gripper:
  • Have a range of opening from 0 mm to 85 mm
  • Must grab as small as 1.5 mL tubes
  • Must grab as large as 96-well plates
Centrifuge:
  • Rotate at a speed capable of exerting a minimum gravitational force of 6000G
  • Must be equipped with security devices such as detection of abnormal vibration or securing the lid after closing
MC96:
  • Suitable for a 96-well plate (common sized plate for biological manipulations
  • Control and maintain temperature cycling between 4 to 80℃ ±1.5℃
  • Achieve a cooling and heating ramp of 0.5 to 1℃/s
  • Apply an electromagnetic field on demand
  • Less than 1000$
MC1.5:
  • Suitable for a test tube of 1.5mL
  • Capable of independent control for each unit of three tubes
  • Control and maintain temperature cycling between 4 to 80℃ ±1.5℃
  • Achieve a cooling and heating ramp of 0.5 to 1℃/s
  • Apply an electromagnetic field on demand
  • Less than 1000$
MC1.5:
  • Suitable for a glass tube having a diameter of 25mm, capacity of 50mL
  • Independent control for each tube
  • Control and maintain temperature cycling between 0 to 37℃ ±1.5℃
  • Achieve a heating ramp of 0.08℃/s
  • Achieve a cooling ramp of 0.1℃/s above room's temperature
  • Achieve a cooling ramp of 0.025℃/s below room's temperature
  • Stirring the liquid (Mixing of bacterial cultures)
  • Calculate the optical density of the liquid with a precision of ±5% from a reference turbidimeter
  • Less than 1000$
 

Future Plans