Difference between revisions of "Team:SPSingapore/Notebook-Week-4"
| Line 128: | Line 128: | ||
<center> | <center> | ||
<table style = "width:750px; border-collapse: collapse;"> | <table style = "width:750px; border-collapse: collapse;"> | ||
| + | |||
<tr class = "tablenotebook"><td><p class = "paper"> | <tr class = "tablenotebook"><td><p class = "paper"> | ||
| − | + | May 25 | |
<br><br> | <br><br> | ||
| − | < | + | <font style = "color:blue;font-weight:bold;font-size:11pt;">☀ Yi Han ☀</font> |
<br><br> | <br><br> | ||
| − | & | + | ❱❱❱ Received 2 bacterial stab cultures, EsaR/I plasmid from addgene (CHL) and BBa_K299812 from iGEM HQ. |
<br> | <br> | ||
| − | & | + | ❱❱❱ Streaked out on plates with amp. |
<br><br> | <br><br> | ||
</p> | </p> | ||
| + | <div style = "border-top: 2px solid turquoise;"></div> | ||
</td> | </td> | ||
</tr> | </tr> | ||
| − | |||
<tr class = "tablenotebook"><td><p class = "paper"> | <tr class = "tablenotebook"><td><p class = "paper"> | ||
May 26 | May 26 | ||
<br><br> | <br><br> | ||
| − | < | + | <font style = "color:blue;font-weight:bold;font-size:11pt;">☀ Adrian ☀</font> |
<br><br> | <br><br> | ||
| − | & | + | ❱❱❱ Transformed:<br> |
+Kit plate 1 9N Ba_K763002 chl<br> | +Kit plate 1 9N Ba_K763002 chl<br> | ||
+Kit plate 4 13L BBa_E0040 amp | +Kit plate 4 13L BBa_E0040 amp | ||
<br> | <br> | ||
| − | & | + | ❱❱❱ DNa was received in powder form in plates, and resuspended in 10ul ultrapure H2O respectively. Plates were stored in -20/ |
<br><br> | <br><br> | ||
| − | < | + | <font style = "color:blue;font-weight:bold;font-size:11pt;">☀ Yi Han ☀</font> |
<br><br> | <br><br> | ||
| − | & | + | ❱❱❱ Plates cracked in incubator as they dried from lack of humidity. |
<br> | <br> | ||
| − | & | + | ❱❱❱ Transfer to small incubator with beaker of water for humidity no single colonies for inv plasmid -> streak again. |
<br> | <br> | ||
| − | & | + | ❱❱❱ Wrong antibiotic for EsaR/I plasmid → streak out again |
| + | <br><br> | ||
</p> | </p> | ||
| + | <div style = "border-top: 2px solid turquoise;"></div> | ||
</td> | </td> | ||
</tr> | </tr> | ||
| Line 168: | Line 171: | ||
May 28 | May 28 | ||
<br><br> | <br><br> | ||
| − | < | + | <font style = "color:blue;font-weight:bold;font-size:11pt;">☀ Yi Han ☀</font> |
<br><br> | <br><br> | ||
| − | & | + | ❱❱❱ Inoculate single colony of invasin plasmid carrying bacteria in 3mL LB+amp |
<br> | <br> | ||
| − | & | + | ❱❱❱ Transformation of 13L repeated with 1ul of DNA. |
</p> | </p> | ||
| + | <br><br> | ||
| + | <div style = "border-top: 2px solid turquoise;"></div> | ||
</td> | </td> | ||
</tr> | </tr> | ||
| Line 180: | Line 185: | ||
May 29 | May 29 | ||
<br><br> | <br><br> | ||
| − | < | + | <font style = "color:blue;font-weight:bold;font-size:11pt;">☀ Xin Yi ☀</font> |
<br><br> | <br><br> | ||
| − | & | + | ❱❱❱ No colonies grew for 13L on all plates → adjust incubator, make new media |
<br> | <br> | ||
| − | & | + | ❱❱❱ Miniprep of inoculated bacteria for invasin plasmid, incubate sample in 37degC for 1h 20min: |
<br><br> | <br><br> | ||
<div> | <div> | ||
| Line 204: | Line 209: | ||
</div> | </div> | ||
<br> | <br> | ||
| − | <div class = "nbafter">& | + | <div class = "nbafter">❱❱❱ Repeat transformation of 13L with 1ul</div> |
<br><br> | <br><br> | ||
</p> | </p> | ||
| + | <div style = "border-top: 2px solid turquoise;"></div> | ||
</td> | </td> | ||
</tr> | </tr> | ||
| Line 213: | Line 219: | ||
May 30 | May 30 | ||
<br><br> | <br><br> | ||
| − | < | + | <font style = "color:blue;font-weight:bold;font-size:11pt;">☀ Xin Yi & Yi Han ☀</font> |
<br><br> | <br><br> | ||
| − | & | + | ❱❱❱ Miniprep of EsaR/I plasmid for 4 colonies |
| − | + | ||
| − | + | ||
<br><br> | <br><br> | ||
| − | < | + | ❱❱❱ Restriction digest for EsaR with XbaI/BamHI: |
| − | < | + | <br><br> |
| − | < | + | <span> |
| − | < | + | <b><u>RE reaction</u></b><br> |
| − | + | 5ul plasmid<br>5ul buffer<br>1ul XbaI<br>1ul BamHI<br>Add H2O to 50ul | |
| − | + | </span> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | </ | + | |
<br><br> | <br><br> | ||
</p> | </p> | ||
Revision as of 17:12, 13 September 2015
Research Notebook |
Week 1 (24/5 - 30/5)
|
