Difference between revisions of "Team:Marburg/Labbook/Curli"
| Line 409: | Line 409: | ||
<tr> | <tr> | ||
<td class='col'>OD</td> | <td class='col'>OD</td> | ||
| − | <td class='col'></td> | + | <td class='col'>0,7117</td> |
| − | <td class='col'></td> | + | <td class='col'>0,2091</td> |
| − | <td class='col'></td> | + | <td class='col'>0,2882</td> |
| − | <td class='col'></td> | + | <td class='col'>0,1249</td> |
| − | <td class='col'></td> | + | <td class='col'>0,4069</td> |
| − | <td class='col'></td> | + | <td class='col'>0,0909</td> |
| − | <td class='col'></td> | + | <td class='col'>0,0379</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class='col'>fluorescence</td> | <td class='col'>fluorescence</td> | ||
| − | <td class='col'></td> | + | <td class='col'>3850</td> |
| − | <td class='col'></td> | + | <td class='col'>229</td> |
| − | <td class='col'></td> | + | <td class='col'>303</td> |
| − | <td class='col'></td> | + | <td class='col'>203</td> |
| − | <td class='col'></td> | + | <td class='col'>1297</td> |
| − | <td class='col'></td> | + | <td class='col'>342</td> |
| − | <td class='col'></td> | + | <td class='col'>225</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class='col'> | + | <td class='col'>F/OD</td> |
| − | <td class='col'></td> | + | <td class='col'>5410</td> |
| − | <td class='col'></td> | + | <td class='col'>1095</td> |
| − | <td class='col'></td> | + | <td class='col'>1051</td> |
| − | <td class='col'></td> | + | <td class='col'>1625</td> |
| − | <td class='col'></td> | + | <td class='col'>3188</td> |
| − | <td class='col'></td> | + | <td class='col'>3762</td> |
<td class='col'></td> | <td class='col'></td> | ||
</tr> | </tr> | ||
Revision as of 19:23, 13 September 2015
15/04/28
GXL-PCR
Used primers:
Promotor (plac): IC1 + IC2
csgA: IC3 + IC4
RBS + mCherry: IC5 + IC6
Backbone: IC7 + IC8
15/05/07
Resuspending of speedvaced gel extracted plac, mCherry, Curly
high values (100g/µl-250ng/µl) but weird curves
CPEC
15/05/08
Gel of CEPC reaction
No clear band visible, with a lot of imagination there is a band, which would be right in size -> transform anyway
Transformation of CEPC Curly
--> next day: colonies are visible
15/06/08
Overnightcultures
W3110 DcsagA cells in 1 mL LB-Media
eCsgAa-Cells in 1 mL LB-Cm-Media
15/06/09
Overdayculture
W3110 DcsagA + pC1 in 3 mL LB-Cm-Media (1% Glucose)
Platereader experiment
1. Add 30 µL overnight culture (probe B1 in the 96-well-plate)
2. After 2 h: induction with IPTG (probe B2 in the 96-well-plate)
3. After 2 h: fluorescence measurement with the plate reader
4. Non fluorescent cells (Control)
5. culture 4h after induction
6. culture 4h after induction 1:10 diluted
| 1 | 2 | 3 | 4 | 5 | 6 | blank | |
| OD | 0,7117 | 0,2091 | 0,2882 | 0,1249 | 0,4069 | 0,0909 | 0,0379 |
| fluorescence | 3850 | 229 | 303 | 203 | 1297 | 342 | 225 |
| F/OD | 5410 | 1095 | 1051 | 1625 | 3188 | 3762 |
TEXT
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