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The Project
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        Policy & Practices
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<h2>What we do</h2>
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      <h2 class="ui inverted header">UFSCar-Brasil Team impacting society</h2>
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<a href="#overview" class="ui huge inverted primary basic button">Overview<i class="right arrow icon"></i></a>
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      <h3 class="ui header" id="overview">Overview</h3>
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      <p>The set of P & P activities represents all dimensions reached by project developed by iGEM teams. Brasil-UFSCar team held a series of activities that explored different aspects that bug-shoo reached in society. Since dengue affects all social classes and the solution lies in solving social, political and educational problems, our main motivation was to address the diversity that characterizes our society and the various problems related to dengue. So we developed activities with children from a poor community and we had the opportunity to know the reality of public service at our City Hall. We also deepened our involvement in education, promoting interactions between academic community and basic education, discussing the role of the University in social demands. We knew administrative sectors of UFSCar and created ways to raise money. We debated with other Brazilian teams. We also prepared a law project which provides free distribution of less toxic repellents without DEET. Finally, we reflected on aspects of our project that may be relevant to other teams, and how our experience can contribute to the formation of new iGEM teams. We hope our experiences can contribute to the consolidation of synthetic biology in Brazil. </p>
 
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<h3 class="ui header" id="overview">Overview</h3>
 
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<p>Diseases transmitted by insect vectors, such as malaria and dengue, affect significantly the Brazilian population. In our city, São Carlos, this year in summer the public health organ featured a big number of cases. Working in this problem,
 
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our project consists in the development of an alternative repellent of that currently sold in the market and more or equally effective in preventing mosquitoes bites transmitted diseases. The main compound in current repellents is DEET (N,
 
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N-diethyl-m-toluamide), a toxic molecule which at certain concentrations could be lethal, and therefore must have strict control on their use in products. The main characteristic of our repellent is the long duration when compared to other
 
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products and the replacement of the compound DEET by D-limonene. Instead, the D-limonene has low toxicity, is highly volatile and present a pleasant smell.</p>
 
  
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<p>Our proposal is building a bacteria carrying out the production of D-limonene via limonene synthase. To enable long term storage at room temperatures, the bacterial cells that make up the repellent will be plasmolyzed, with suspended metabolism,
 
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considered a dormant state. The maintenance of this state will be obtained by a solution of polyethyleneglycol (PEG) that will raise the osmotic pressures. Once in contact with the skin, the PEG solution will be diluted by sweat, inducing
 
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an osmotic shock in cells. Then the universal stress protein promoter (UspA) will be activated and will induce the expression of limonene synthase. Beyond PEG, other compounds such as glycerol and metal ions in low concentrations will compose
 
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our insect repellent cream, in order to sustain the bacteria to the required metabolites building and posterior enzyme activities. In this way, the distribution of our repellent may become feasible, allowing its use.</p>
 
  
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<p>To overcome those problems with limonene synthase folding, we used constitutive promoters for the expression of chaperones from all Escherichia coli available classes (like ClpB, DnaK and IbpA / IbpB). It will reinforce the stockpile of chaperones
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naturally produced during osmotic shocks (heat-shock proteins) in bacteria. Our goal is to improve protein solubility, indirectly creatinga toolkit for protein solubility enhancement for future iGEM teams. . Besides, reducing the occurrence
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of insoluble bodies and improving the production of limonene.</p>
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      <img class="ui small image" src="https://static.igem.org/mediawiki/2015/e/e3/Political.png" alt=""> Political
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    </h2>
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    <p>The team proposed a law project that aims for the free distribution of repellents without DEET. We also held a debate with professionals from different fields, which helped us to discuss limitations and different aspects of our project. Read more</p>
 +
    <div class="ui hidden divider"></div>
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    <div class="center aligned">
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      <a href="https://2015.igem.org/wiki/index.php?title=Team:UFSCar-Brasil/political.html" class="ui button">See more!</a>
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    <h2 class="ui header">
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      <img class="ui small image" src="https://static.igem.org/mediawiki/2015/2/27/UFSCar-Brasil_social.PNG" alt=""> Social
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    </h2>
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    <p>Activity in the Sao Carlos VIII (a poor neighborhood) aimed to educate children about the relation between dump and dengue. The team presented the play "Mighty bacteria against dengue", covering the main aspects of the disease, prevention and the
 +
      role of science in solving problems.</p>
 +
    <div class="ui hidden divider"></div>
 +
    <div class="center aligned">
 +
    <a href="https://2015.igem.org/wiki/index.php?title=Team:UFSCar-Brasil/social.html" class="ui button">See more!</a>
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<p>Finally, a way to ensure the biosafety in the use of our repellent and keep a control of bacterial cells is the use of a system of programmed death or Kill Switch. This system will work with two suicide genes: Killer red, a protein which trigger
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oxidative species generation destroying the DNA (plasmid and chromosomal) allied to a potent RNAse which will avoid any contribution of RNA to host or normal microbiota. These genes will be switch on through an association with a zinc sensitive
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promoter triggered by zur proteins which associate with zinc and avoid the expression of them allowing the bacterial growing. After long periods of repellent action, bacteria activities will decrease zinc concentration in medium, which allows
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      <img class="ui small image" src="https://static.igem.org/mediawiki/2015/9/91/Educacao.PNG" alt=""> Educacional
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the gene activation and cells death.</p>
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    </h2>
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    <p>On several occasions we present synthetic biology and its applications to groups with different educational levels. We launched a Seminar Cycle for academic community of the University, we offer workshops and presented our project at scientific meetings.
 +
      Read more Read more</p>
 +
    <div class="ui hidden divider"></div>
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    <div class="center aligned">
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      <a href="https://2015.igem.org/Team:UFSCar-Brasil/educacional.html" class="ui button">See more!</a>
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    </div>
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  <div class="column">
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    <h2 class="ui header">
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      <img class="ui small image" src="https://static.igem.org/mediawiki/2015/9/9d/Meio-ambiente.PNG" alt=""> Safety/Security
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    </h2>
 +
    <p>To ensure that limonene was actually a compound of low toxicity compared to DEET, we conducted a simple experiment using beans that received different treatments. </p>
 +
    <div class="ui hidden divider"></div>
 +
    <div class="center aligned">
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      <a href="https://2015.igem.org/wiki/index.php?title=Team:UFSCar-Brasil/environmental.html" class="ui button">See more!</a>
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    <h2 class="ui header">
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      <img class="ui small image" src="https://static.igem.org/mediawiki/2015/8/8d/Biobricks.PNG" alt=""> Problems
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    </h2>
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    <p>The problems with BioBricks delivery and possible solutions that would make this procedure easier. See how our team has overcome this problem. Read more.</p>
 +
    <div class="ui hidden divider"></div>
 +
    <div class="center aligned">
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    <a href="https://2015.igem.org/wiki/index.php?title=Team:UFSCar-Brasil/biobricks.html" class="ui button">See more!</a>
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    </div>
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    <h2 class="ui header">
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      <img class="ui small image" src="https://static.igem.org/mediawiki/2015/8/87/UFSCar-Brasil_meetings.png" alt="">Meetings
 +
    </h2>
 +
    <p>Three Brazilian participating teams of the iGEM 2015 assembled at Jamboré Brazil, in July. The interaction between the teams allowed the discussion of the projects, discussion of proposals and challenges that the teams will possibly face in the Giant
 +
      Jamboree. Read more. </p>
 +
    <div class="ui hidden divider"></div>
 +
    <div class="center aligned">
 +
      <a href="https://2015.igem.org/wiki/index.php?title=Team:UFSCar-Brasil/meeting.html" class="ui button">See more!</a>
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      <img class="ui middle aligned small image" src="https://static.igem.org/mediawiki/2015/2/23/UFSCar-Brasil_funds.png" alt=""> Fundraising
 +
    </h2>
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    <p>Our Team raised money in many ways to fund experiments, registration and travel. Read more. </p>
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    <div class="ui hidden divider"></div>
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    <div class="center aligned">
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      <a href="https://2015.igem.org/wiki/index.php?title=Team:UFSCar-Brasil/fundraising.html" class="ui button">See more!</a>
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Political
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<p>Diseases transmitted by insect vectors, such as malaria and dengue, affect significantly the Brazilian population. In our city, São Carlos, this year in summer the public health organ featured a big number of cases. Working in this problem,
+
−
our project consists in the development of an alternative repellent of that currently sold in the market and more or equally effective in preventing mosquitoes bites transmitted diseases. The main compound in current repellents is DEET
+
−
(N, N-diethyl-m-toluamide), a toxic molecule which at certain concentrations could be lethal, and therefore must have strict control on their use in products. The main characteristic of our repellent is the long duration when compared
+
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to other products and the replacement of the compound DEET by D-limonene. Instead, the D-limonene has low toxicity, is highly volatile and present a pleasant smell.</p>
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Social
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<p>Diseases transmitted by insect vectors, such as malaria and dengue, affect significantly the Brazilian population. In our city, São Carlos, this year in summer the public health organ featured a big number of cases. Working in this problem,
+
−
our project consists in the development of an alternative repellent of that currently sold in the market and more or equally effective in preventing mosquitoes bites transmitted diseases. The main compound in current repellents is DEET
+
−
(N, N-diethyl-m-toluamide), a toxic molecule which at certain concentrations could be lethal, and therefore must have strict control on their use in products. The main characteristic of our repellent is the long duration when compared
+
−
to other products and the replacement of the compound DEET by D-limonene. Instead, the D-limonene has low toxicity, is highly volatile and present a pleasant smell.</p>
+
−
</div>
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Educacional
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<div class="content">
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<p>Diseases transmitted by insect vectors, such as malaria and dengue, affect significantly the Brazilian population. In our city, São Carlos, this year in summer the public health organ featured a big number of cases. Working in this problem,
+
−
our project consists in the development of an alternative repellent of that currently sold in the market and more or equally effective in preventing mosquitoes bites transmitted diseases. The main compound in current repellents is DEET
+
−
(N, N-diethyl-m-toluamide), a toxic molecule which at certain concentrations could be lethal, and therefore must have strict control on their use in products. The main characteristic of our repellent is the long duration when compared
+
−
to other products and the replacement of the compound DEET by D-limonene. Instead, the D-limonene has low toxicity, is highly volatile and present a pleasant smell.</p>
+
−
</div>
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<div class="title">
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<img class="ui middle aligned tiny image" src="img/icons/meio-ambiente.png" alt="">
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Environment
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<div class="content">
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<p>Diseases transmitted by insect vectors, such as malaria and dengue, affect significantly the Brazilian population. In our city, São Carlos, this year in summer the public health organ featured a big number of cases. Working in this problem,
+
−
our project consists in the development of an alternative repellent of that currently sold in the market and more or equally effective in preventing mosquitoes bites transmitted diseases. The main compound in current repellents is DEET
+
−
(N, N-diethyl-m-toluamide), a toxic molecule which at certain concentrations could be lethal, and therefore must have strict control on their use in products. The main characteristic of our repellent is the long duration when compared
+
−
to other products and the replacement of the compound DEET by D-limonene. Instead, the D-limonene has low toxicity, is highly volatile and present a pleasant smell.</p>
+
−
</div>
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BioBricks
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<div class="content">
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<p>Diseases transmitted by insect vectors, such as malaria and dengue, affect significantly the Brazilian population. In our city, São Carlos, this year in summer the public health organ featured a big number of cases. Working in this problem,
+
−
our project consists in the development of an alternative repellent of that currently sold in the market and more or equally effective in preventing mosquitoes bites transmitted diseases. The main compound in current repellents is DEET
+
−
(N, N-diethyl-m-toluamide), a toxic molecule which at certain concentrations could be lethal, and therefore must have strict control on their use in products. The main characteristic of our repellent is the long duration when compared
+
−
to other products and the replacement of the compound DEET by D-limonene. Instead, the D-limonene has low toxicity, is highly volatile and present a pleasant smell.</p>
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Meeting
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<div class="content">
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<p>Diseases transmitted by insect vectors, such as malaria and dengue, affect significantly the Brazilian population. In our city, São Carlos, this year in summer the public health organ featured a big number of cases. Working in this problem,
+
−
our project consists in the development of an alternative repellent of that currently sold in the market and more or equally effective in preventing mosquitoes bites transmitted diseases. The main compound in current repellents is DEET
+
−
(N, N-diethyl-m-toluamide), a toxic molecule which at certain concentrations could be lethal, and therefore must have strict control on their use in products. The main characteristic of our repellent is the long duration when compared
+
−
to other products and the replacement of the compound DEET by D-limonene. Instead, the D-limonene has low toxicity, is highly volatile and present a pleasant smell.</p>
+
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</div>
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<div class="title">
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<i class="dropdown icon"></i>
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Fundraising
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<div class="content">
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<p>Diseases transmitted by insect vectors, such as malaria and dengue, affect significantly the Brazilian population. In our city, São Carlos, this year in summer the public health organ featured a big number of cases. Working in this problem,
+
−
our project consists in the development of an alternative repellent of that currently sold in the market and more or equally effective in preventing mosquitoes bites transmitted diseases. The main compound in current repellents is DEET
+
−
(N, N-diethyl-m-toluamide), a toxic molecule which at certain concentrations could be lethal, and therefore must have strict control on their use in products. The main characteristic of our repellent is the long duration when compared
+
−
to other products and the replacement of the compound DEET by D-limonene. Instead, the D-limonene has low toxicity, is highly volatile and present a pleasant smell.</p>
+
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{{:Team:UFSCar-Brasil/Templates/Footer}}
 
{{:Team:UFSCar-Brasil/Templates/Footer}}

Latest revision as of 02:58, 14 September 2015

Home
Project
Overview
Issue Plasmolysis and Limonene synthase Protein Solubilization Toolkit Kill Switch
Team
Our Team Attributions
Achievements
Overview
Parts Results Judging Criteria
Policy & Practices
Overview
Political Social Educational Safety/Security Meetings Fundraising
Modeling
Overview
Plasmolysis Protein Solubilization Toolkit Kill Switch
Notebook
Protocols Problems Collaborations Safety Acknowledgements

Policy & Practices

UFSCar-Brasil Team impacting society

Overview

The set of P & P activities represents all dimensions reached by project developed by iGEM teams. Brasil-UFSCar team held a series of activities that explored different aspects that bug-shoo reached in society. Since dengue affects all social classes and the solution lies in solving social, political and educational problems, our main motivation was to address the diversity that characterizes our society and the various problems related to dengue. So we developed activities with children from a poor community and we had the opportunity to know the reality of public service at our City Hall. We also deepened our involvement in education, promoting interactions between academic community and basic education, discussing the role of the University in social demands. We knew administrative sectors of UFSCar and created ways to raise money. We debated with other Brazilian teams. We also prepared a law project which provides free distribution of less toxic repellents without DEET. Finally, we reflected on aspects of our project that may be relevant to other teams, and how our experience can contribute to the formation of new iGEM teams. We hope our experiences can contribute to the consolidation of synthetic biology in Brazil.

Political

The team proposed a law project that aims for the free distribution of repellents without DEET. We also held a debate with professionals from different fields, which helped us to discuss limitations and different aspects of our project. Read more

See more!

Social

Activity in the Sao Carlos VIII (a poor neighborhood) aimed to educate children about the relation between dump and dengue. The team presented the play "Mighty bacteria against dengue", covering the main aspects of the disease, prevention and the role of science in solving problems.

See more!

Educacional

On several occasions we present synthetic biology and its applications to groups with different educational levels. We launched a Seminar Cycle for academic community of the University, we offer workshops and presented our project at scientific meetings. Read more Read more

See more!

Safety/Security

To ensure that limonene was actually a compound of low toxicity compared to DEET, we conducted a simple experiment using beans that received different treatments.

See more!

Problems

The problems with BioBricks delivery and possible solutions that would make this procedure easier. See how our team has overcome this problem. Read more.

See more!

Meetings

Three Brazilian participating teams of the iGEM 2015 assembled at Jamboré Brazil, in July. The interaction between the teams allowed the discussion of the projects, discussion of proposals and challenges that the teams will possibly face in the Giant Jamboree. Read more.

See more!

Fundraising

Our Team raised money in many ways to fund experiments, registration and travel. Read more.

See more!

Our amazing sponsors!

Our amazing sponsors!

Links

iGEM UFSCar

About

Team

Thanks for reading our wiki!

Come Again :)